Post Lab Report Experiment 3 - Chromatography – Analyzing Analgesics by TLC and Isolation of β–Carotene by Column Chromatography Chemicals 1. Acetaminophen (C8H9NO2) 2. Aspirin (C9H8O4) 3. Caffeine (C8H10N4O2) 4. Ibuprofen (C13hH18O2) Introduction In this experiment‚ several analgesics were analyzed by Thin Layer Chromatography (TLC) and the composition of an unknown tablet was identified. We define chromatography as the separation of two or more compounds or ions by
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Analysis of Ethanol in Moonshine | Using the GC-MS | Jennifer Greene | ------------------------------------------------- 4 May 2012 ------------------------------------------------- 4 May 2012 Introduction: In this experiment gas chromatography is applied to separate the water-ethanol mixture. This method is often used in the determination of alcohol in blood or urine. One obvious application is when law enforcement agencies need to determine whether or not someone is inebriated. In
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Outline the role of a chemist employed in a named industry or enterprise‚ identifying the branch of chemistry undertaken by the chemist and explaining a chemical principle that the chemist uses Burhan Gemikonakli is a Plant Chemist at Qenos‚ a major Australian chemical manufacturing company located at Botany‚ NSW. The role of a plant chemist working in the polymer industry is concerned with the conversion of ethane to ethylene and the polymerisation of ethylene to polyethylene. Examples of
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from Tomato Paste Goal / Purpose To extract the carotenoid pigments from canned tomato paste and separate them by column chromatography to obtain a solution that contains lycopene. Then record the ultraviolet-visible spectrum of this solution and analyze it for evidence of isomerization. Calculation List of Materials 4.0g tomato paste screw cap vial Chromatography column 25ml buret 50ml flask ring stamd and clamp 50% mixture of acetone and low boiling petroleum ether 5ml extraction
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Extraction * 6 Precipitation and differential solubilization * 7 Ultracentrifugation * 8 Chromatographic methods * 8.1 Size exclusion chromatography * 8.2 Separation based on charge or hydrophobicity * 8.3 Ion exchange chromatography * 8.4 Affinity chromatography * 8.4.1 Metal binding * 8.4.2 Immunoaffinity chromatography * 8.4.3 Purification of a tagged protein * 8.5 HPLC * 9 Concentration of the purified protein * 9.1 Lyophilization
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CHROMOTOGRAPHY Chromatography is used to separate mixtures of substances into their components. All forms of chromatography work on the same principle that they all have a stationary phase (a solid or a liquid supported on a solid) and a mobile phase where liquid or a gas is involved. The mobile phase flows through the stationary phase and carries the components of the mixtures with it. Different components travel at different rates. In paper chromatography‚ the stationary phase is a very uniform
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Title of experiment 3: Gel Filtration Chromatography of LDH INTRODUCTION Gel filtration chromatography is a type of column chromatography in which separated protein‚ peptides and amino acids on their molecular size. The stationary phase consists of beads containing pores. The mobile phase is the solvent that is found both around the beads and in the pores of the stationary phase matrix. As the sample is passes through the column‚ the molecule that are larger than the pores will not retarded by
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High-performance liquid chromatography (formerly referred to as high-pressure liquid chromatography)‚ HPLC‚ is a chromatographic technique used to separate the components in a mixture‚ to identify each component‚ and to quantify each component. HPLC is considered an instrumental technique of analytical chemistry (as opposed to a gravimetric technique). In general‚ the method involves a liquid sample being passed over a solid adsorbent material packed into a column using a flow of liquid solvent.
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Biochemistry Exam I Terminology Boiling point is the temperature at which the vapor pressure of the liquid equals the pressure surrounding the liquid. At this temperature the liquid phase changes into the gaseous phase (also a colligative property) Melting point is the temperature at which the solid and liquid phases are in equilibrium. At this temperature the solid state changes into a liquid phase (also a colligative property) Specific heat is the amount of energy (in joules or calories) needed
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al.Qaimare Rifqa Shawer Lab report 5 21-3-2013 Adsorption Chromatography Introduction: The technique of adsorption chromatography may be illustrated by a description of how two dyes are separated when a solution of the dyes is passed through a column of powdered silica contained in a vertical glass tube‚ it’s a method used to separate solids or liquids. At the first the two dyes are adsorbed at the top of the column‚ giving this region a nearly dark blue color. However‚ when the flow
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