yielding a 95.8% recovery rate. As for the organic compound‚ 0.166g of fluorene was secured and its respective melting point range was 116.0 C – 117.0 C. The identity of final acidic product was revealed through the comparison in melting points of the three known products. The melting point range matched up similarly with 3-chlorobenzoic acid. The experiment began with 0.210g of the mixture of an unknown acid and fluorene. The yield in products
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Beam Chopping corrects for flame emission but not for scattering. Can distinguish the signal of the flame from the atomic line at the same wavelength. The higher the absorbance the higher the transmittance. Intro to IR Near IR- (0.78 to 2.8 micrometers) Mid IR- (2.5- 50 micrometers) Far IR- (50-1000 micrometers) Most common region used is Mid IR btwn 2.5- 15 micrometers Mid IR: based on diffracting gratings but now current instrumentation are of Fourier transform type Far-IR: useful
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made me think that we did something wrong and our results would not be accurate. Crystallization: Part B: Start Finish % Recovery Expected Remaining Sulfanilamide 0.093g 0.059g 68.6% 0.034g Part C: Start Finish % Recovery Expected Remaining Fluorene 0.044g 0.018g 48.6% 0.026g Melting Points: Compound Literature Melting
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CH3I Before coming to lab‚ please review the following techniques: "Reluxing a reaction‚" "Extraction and washing‚" "Drying an Organic Solvent‚" "Evaporating an Organic Solvent‚" "Analyzing a Solution by Thin Layer Chromatography (TLC)‚" "Separating Mixtures by Column Chromatography‚" and "Characterizing Compounds by IR." Notice that this lab brings together most of the techniques that we have used this semester. Then complete the following table before coming to class so that you will be prepared
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seen at the top of the solution. Lead Acetate Cloudy solution with small specks of white precipitate. Ice cold ethanol Cloudy at the bottom and about 0.5 cm of the top of the solution was clear. Table 4: The results of Gel Filtration Chromatography Eluate Component Volume collected in mL First Blue dextrin 3.00 Second Brown cytochrome 7.00 Third Clear phosphate buffer 5.00 Table 5: The result of Electrophoresis of Amino Acids Distance
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ORGANIC CHEMISTRY 1 LAB EXPERIMENT NO. 1 to 6 DISCUSSION EXPERIMENT NO. 1: Mel=ng Points And Boiling Points Of Organic Compounds Mel=ng Point -‐ temperature at which the liquid and solid are in equilibrium at a pressure of 1 atm Mel=ng Point Range -‐ determines the purity of a solid sample -‐ temp at
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High Performance Liquid Chromatography Analysis of Aspirin Problem: Was aspirin (acetylsalicylic acid) successfully synthesized? Are there impurities or by-products present in the synthesized aspirin? How pure is the synthesized aspirin? Introduction: In the last experiment‚ aspirin was synthesized followed by characterization of the product using several different techniques. Melting point was a test that provided information about the identity and purity of the aspirin product. The iron(III)chloride
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crystals started to melt in the fast ramp. The end temperature was 5°C above the end temperature in the fast ramp‚ and the ramp rate was 1°C /min. After running the slow ramp the melting point range was compared to possible known compound. The compound Fluorene was a possible match for what the unknown compound was. To confirm that the unknown compound was Flourene a mixed melting point was ran. In a weighing paper the unknown compound and flouerene was mixed and 1-2mm was placed in a capillary tube. A
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room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded and the column was washed twice (i.e. centrifuged); first in 30 mL of low salt Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl)‚ followed by 30 mL of high salt Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 0.2 M KCl). Each time after adding the buffers‚ PMSF (protease
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MCOPS‚ Manipal ANALYTICAL METHODS FOR RESIDUAL SOLVENTS INTRODUCTION: Organic solvents are routinely applied during synthesis of drug substances‚ excipients‚ or during drug product formulation. They are not desirable in the final product‚ mainly because of their toxicity‚ their influence on the quality of crystals of the drug substance and their odor or taste‚ which can be unpleasant for patients. To remove them‚ various manufacturing processes or techniques (usually under
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