filtration GEL FILTRATION OF PROTIENS Aim: The aim of this experiment is to identify proteins from a complex mixture using the gel filtration technique also known as size exclusion chromatography. This technique is widely used by biochemists when proteins larger than the pores are excluded from the column and the smaller molecules elute last and then collected in test tubes for examination by spectroscopic techniques. The red/brown proteins‚ in particular‚ will be observed closely as they
Premium Molecular biology Protein Chromatography
Purpose The purpose of this experiment is to exemplify how differences in molecular weight allow separation of polymers from their monomers. Methods of dialysis and gel filtration chromatography will be used to separate a glucose monomer from a starch polymer. Colorimetric glucose oxidase assay will be used to monitor the presence of glucose and a colorimetric iodine assay will be used to monitor the presence of starch in prepared solutions
Premium Gas chromatography Chromatography Analytical chemistry
IDENTIFICATION OF UNKNOWNS BY TLC AND MP IN COMBINATION Introduction: Thin layer chromatography (TLC) is one of the most valuable techniques in organic chemistry. This is a best method of separating and identifying mixtures of two or more compounds. The separation is accomplished by the distribution of the mixture between two phases: one that is stationary and one that is moving or mobile. Chromatography works on the principle that different compounds will have different solubilities and adsorption
Free Chromatography Thin layer chromatography
with rhamnose in a bacterial system suitable for the RNA T7 polymerase. The recombinant protein will be isolated‚ purified and characterized by SDS-PAGE and Western blot. Several chromatographic techniques including gel filtration and affinity chromatography will also be employed for the purification of the recombinant human PDGF-B. Final Report Submission BIND 5005-Fall 2009 INTRODUCTION. The PDGF family consists mainly of four isoforms called as PDGF-A‚ PDGF-B‚ PDGF-C and PDGF-D‚ where
Premium Molecular biology DNA Gene expression
for H. sp. KHS3. To our knowledge‚ most of the studies on Halomonas sp. focused on degradation of monoaromatic rather than polycyclic and heterocyclic compounds. We have demonstrated that H. sp. KHS3 has the ability to growth using phenanthrene‚ fluorene and naphthalene as the only carbon source. Similar ability has been demonstrated by
Premium Archaea Carbon Bacteria
experiment we will learn microscale level lap techniques such as vacuum filtration‚ column chromatography‚ and centrifugation to extract the active ingredient from tablets. Separation and Purification Scheme: Analgesic with Binder Add methanol (CH3OH) dissolve analgesic Analgesic in solution with binder Centrifuge +filter some binder Analgesic in solution with binder Alumina column remaining binder Analgesic in solution with methanol Evaporate most
Premium Paracetamol Aspirin Ibuprofen
ethanol‚ so the cooler surface of the sponge helped condense 1-butanol‚ but the ethanol still made it to the vial because of its lower boiling point. The sponge helped improve efficiency of the distillation. We will use distillation plot and gas chromatography to test it. To draw the distillation curve graph ‚ we counted the number of drops and temperature. The graph shows us the specific spots (large number drops) have a little change in temperature.it means that a compound has been distilled off
Premium Distillation Boiling point
Buckminster Fuller Contribution Disadvantages of Technique Define the following: The Cell: Optical tweezers: Fluorochrome: Electron Tomography: Centrifugation: Differential Centrifugation: Equilibrium Sedimentation: Homogenate: Electrophoresis: Column Chromatography: Isoelectric Focusing: Western Blotting: Flow Cytometry: 2-D surface metabolism: Tensegrity: Answers the following questions: What is the difference between Resolution and Magnification? What is the difference between Amplitude contrast
Premium Cell Protein Endoplasmic reticulum
source of antioxidant‚ has been demanded due to its biological and physiochemical properties. Therefore‚ there is a need to develop the form of Lycopene supplement. Lycopene was extracted from tomato wastes by using acetone and purified by column chromatography. The extract was characterized by UV-Vis spectrophotometer. An average recovery of 40 mg lycopene / kg tomato wastes was obtained. Lyco-cookies were prepared by using common ingredients containing 3 mg lycopene per serving. Introduction
Premium Lycopene Tomato Antioxidant
Final Organic Study Guide: Be able to develop an Experiment Beginning to End * Find a good reaction * Good reaction is defined by high yield‚ green chemistry (less waste‚ less harmful chemicals) * Reaction found on Reaxys * Find MSDS Sheet for all Compounds (Reactants‚ Products and Side Products) * It is important to know what you are working with * The toxicity category numbers are there to gauge how toxic the chemical is‚ 4 being most severe and 1 being least
Premium Distillation Solubility Solvent