Appearance of solution. Dissolve 1.0 g in 9 ml of alcohol R. The solution is clear (2.2.1) and colourless (2.2.2‚ Method II). Related substances. Examine by liquid chromatography (2.2.29). Prepare the solutions immediately before use. Test solution. Dissolve 0.10 g of the substance to be examined in acetonitrile for chromatography R and dilute to 10.0 ml with the same solvent. C. N‚N′-diacetyl-L-cystine‚ Reference solution (a). Dissolve 50.0 mg of salicylic acid R in the mobile phase and dilute
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Analysis: 1. What volume of ammonium sulphate was required to give rise to precipitation? Tube 1: 5.5mls of (NH₄)₂SO₄ was required to give rise to precipitation. 5.5ml/20ml *100% = 27.5% Tube 2: 6.7mls of (NH₄)₂SO₄ was required to give rise to precipitation. 6.7ml/20ml *100% = 33.5% Tube 3: 5.6mls of (NH₄)₂SO₄ was required to give rise to precipitation. 5.6ml/20ml *100% = 28.0% Tube 4: 6.2mls of (NH₄)₂SO₄ was required to give rise to precipitation. 6.2ml/20ml *100% = 30.5% 1. From the
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Separation of Amino Acids by Paper Chromatography Chromatography is a common technique for separating chemical substances. The prefix “chroma‚” which suggests “color‚” comes from the fact that some of the earliest applications of chromatography were to separate components of the green pigment‚ chlorophyll. You may have already used this method to separate the colored components in ink. In this experiment you will use chromatography to separate and identify amino acids‚ the building blocks of proteins
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Spearmint and Caraway Oils: (+)- and (-) – Carvones Purpose Compare (+)- carvone from caraway oil to (-)- carvone from spearmint oil‚ using gas chromatography. Experimental Odor: Smell containers of spearmint and caraway oil and two carvones. Analytical Gas Chromatography: Put five individual samples by different group members into Gas Chromatograph to calculate the percent composition. Claudia tested S- (+)- carvone and R- (+) – Limonene‚ Daniel tested Spearmint oil (pure)‚ Greg tested R-
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Bromobenzene 155-156 -30.8 1.50 Insoluble Dibromobenzene 220.40 87.31 0.96 Insoluble MATERIALS: Graduated cylinder Weight scale Buchner funnel Filter flask Rubber stopper Hot plate Thermometer Conical funnel Various size beakers Fractionating column (for reflux) Various sized round bottom flasks Distillation head Condenser Vacuum adapter Clamps and stands Test tubes PROCEDURE: Pre Lab: The experiment should be carried out AWAY from the sunlight. Before beginning the experiment
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uses a column that allows many small distillations to occur as the vapor ascends the fractional distillation column. Purpose: In this experiment we aim to demonstrate that we can separate two volatile compounds from a mixture due to the different chemical properties of each compound. We will accomplish this by a separation procedure known as distillation‚ which relies on each compound having a distinct and separate boiling point. Our pure products will be analyzed with gas chromatography to determine
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A SEMINAR ON “SYNTHESIS OF LOW COST ADHESIVES FROM PULP & PAPER INDUSTRY WASTE” For the partial fulfillment of degree of Bachelor of Technology Submitted by DINESH G. TEMBHURNE (20090557) Under the guidance of MISS. NAZLI GITE. DEPARTMENT OF PETROCHEMICAL ENGINEERING DR. BABASAHEB AMBEDKAR TECHNOLOGICAL UNIVERSITY‚ LONERE (RAIGAD) - 402 103 2010-2011 DR. BABASAHEB AMBEDKAR TECHNOLOGICAL UNIVERSITY LONERE - 402 103‚ TAL – MANGAON
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TITLE Crime and Dye Lab Special Projects 1 Martin Enem LAB SECTION: BB2 INTRODUCTION Light is usually viewed as a result of the heating of a substance. The higher the temperature at which the substance is heated‚ the greater the vibrations that lead to certain light intensity given off by the molecule. It is this same theory that suggests why steel glows red hot when heated to high enough temperatures. The process of light emissions can also be induces through other means. 1 One
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solid phase extraction cartridges (PGC)‚ e.g. Thermo Hypersil keystone Hypersep Hypercarb 200 mg‚ 3 ml cartridges‚ from Thermo Hypersil Keystone‚ UK (b) C18 solid phase extraction cartridges‚ e.g. Isolute C18(EC) solid phase extraction columns‚ size 1 g sorbent in 6 ml reservoir from Argonaut Technologies‚ Mid Glamorgan‚ UK (c) Glass-fibre filters (e.g. Whatman GF/C)‚ diameter 25-70mm (d) Argon or nitrogen‚ >99.99% (e) Borosilicate test tubes or vials‚ >3 ml capacity
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CHAPTER ONE 1.0 INTRODUCTION 1.1 Antimycobacterial Studies An Antimycobaterial is a substance that kills or inhibits the growth and activities of disease causing Mycobacteria (e.g.‚ Mycobacterium tuberculosis‚ Mycobacterium Leprae‚ etc) that are responsible for fatal diseases such as Tuberculosis and leprosy. Antimicrobacterials can either kill microbes (microbiocidal) or prevent the growth of microbes (microbiostatic) (Hannan et al.‚ 2011). Mycobacterium is a genius in the family of the Mycobacteriaceae
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