(Dioscorea opposita Thunb) class IV chitinase‚ whose genomic DNA was cloned by Mitsunaga et al. (2004)‚ was produced by the recombinant Pichia pastoris X-33 yields remarkably‚ such as 66 mg/ L of culture medium. The chitinase was purified by column chromatography after Endoglycosidase H treatment and was then characterized. The Pichia-produced chitinase It showed properties that are similar to the original chitinase E purified from the yam tuber‚ as reported by Arakane et al. (2000). This Pichia-produced
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Medicinal Plants Introduction Introduction “In All things there is a poison‚ and there is nothing without a poison. It depends on only upon the dose whether a poison is a poison or not”---------Paracelsus (1493?-1541‚ Switzerland) Chinese saying “Shi Yao San Fei Du” means “Any drugs have toxic effects”. Introduction: Why Study Medicinal Plants Medicinal plants and religions • Medicinal plants have played an essential role in the development of human culture‚ for example religions
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using 1-methylimidazole as the starting material by using the alkylation and metathesis synthesis methods. H1-NMR and Ion Chromatography (IC) analysis will be used to verify the characteristic and presence of impurities to determine the purity of
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Preparation of the propolis extracts: the propolis sample of the Brazilian native bee species Melipona quadrifasciata was obtained in May‚ 2013 in the city of Blumenau‚ SC‚ Brazil (26 ° 54’21.3 "S 49 ° 04’49.1 "W). In order to obtain a hydroalcoholic crude extract (HCE)‚ 284.3 grams of propolis were pulverized and macerated in 70% ethanol (m m-1)‚ left in a dark chamber for 7 days at room temperature‚ filtered in vacuum and taken to complete drying in a rotary evaporator with reduced pressure. In
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amber or non-amber‚ and storage temperatures (4‚ 25 and 40°C) on the stability of 12 extemporaneously prepared Phenytoin oral suspension was evaluated. The concentration of Phenytoin oral suspension was evaluated using high performance liquid chromatography (HPLC)‚ in which the retention time of Phenytoin was detected at 6.3 minutes. Phenytoin oral suspension was found to be stable up to two weeks‚ retaining minimal of 76.5% of Phenytoin. Degradation could have taken place as a lower concentration
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The plant material (10 g) used‚ was a representative sample of C. sativus stigmas obtained from the Cooperative De Safran (Krokos Kozani‚ Greece) in 2012. The ISO 3632-1 (ISO 2011) quality parameters E1%1cm calculated at 440 nm‚ 257 nm‚ and 330 nm for an aqueous extract were 253‚ 86 and 34 respectively‚ indicating that the plant material was of the highest quality (Category I). 2.2. Standards‚ reagents and solvents Safranal (>88%)‚ isophorone (>97%)‚ keto-isophorone (>98%) and 2-phenylethanol (>99%)
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75g of the extract is dissolved in EtOAc and filtered. The filtrate (17.5g) is coated with Celite 545. Silica gel column chromatography (6.9cm i.d. x 35cm) with an n-hexane-EtAOc gradient is performed on the filtrate. A re-chromatograph by silica gel column (2cm i.d. x 40cm) eluted with CHCl3-MeOH gradient is done for the eluate that was produced by the silica gel column chromatography previously. The final eluate‚ 3.07g α-mangostin is produced by CHCl3 and 1.74g γ-mangostin from CHCl3-MeOH. A reversed-phase
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“Shouldn’t you have to pass a urine test to get a welfare check since I have to pass one to earn it for you?” That’s the question many‚ hardworking Americans are asking themselves. In today’s America‚ government aid is highly depended on. The US government has spent $498 billion dollars this year on welfare alone. Mandatory drug testing for welfare applicants is becoming a popular idea across the U.S. Many states including Alabama‚ Kentucky‚ Oklahoma and Louisiana are considering adopting laws that
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4. Results 4.1 Column chromatography The column chromatography was performed using different concentrations of elution solution and the column is eluted in increasing order of polarity of solvent from 100 % of extract‚ 25 % of ethyl acetate in hexane‚ 50 % of ethyl acetate in hexane‚ 75 % of ethyl acetate in hexane and 100 % of
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Carbohydrates General molecular formula Cn(H2O)n Appeared to be hydrates of carbon. not all carbohydrates have this empirical formula: deoxysugars‚ aminosugars Carbohydrate - polyhydroxy aldehyde‚ ketones. General characteristics Most carbohydrates are found naturally in bound form rather than as simple sugars Polysaccharides (starch‚ cellulose‚ inulin‚ gums) Glycoproteins and proteoglycans (hormones‚ blood group substances‚ antibodies) Glycolipids
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