ABSTRACT Rising energy prices and environmental problems led to the increase in alcohol demands in many aspects‚ like in making fuels. Many people are doing researches about making their own product so as to lessen their everyday expenses. Right now‚ Filipino scientists are in think of the search for alternative fuels‚ with the experts from the University of the Philippines in both Diliman and Los Banos campuses searching all over the archipelago for energy sources from the forests to the bottom
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Introduction: The purpose of this lab was to separate and purify a compound. This was achieved using techniques that allowed the extraction of the acid‚ the isolation of the neutral compound and the melting point classification of the neutral compound to test the purity of the sample. Chemical Reactions: HA + OH- A- + H2O R-COOH + OH- R-COO- + H2O Organic Acid Insoluble in H2O Conjugate base Soluble in H2O Procedure: A 0.170g sample consisting of a mixture of 0.110g
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validation were performed by separating EPA 531.1 carbamate pesticide mixture utilizing the LC×LC system‚ repeating 20 time in different days. The LC×LC data was processed in GC×GC software. In the software‚ the chromatogram was smoothed first column by 0.1 and second column by 1.0. For Blob detection function‚ the thresholds were set minimum area as 200‚ minimum volume as 15000 and minimum peak as 140.0 (relative reference). In the chromatogram‚ 6 corresponding peaks (A-F) were detected by the above mentioned
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COLUMN AND THIN LAYER CHROMATOGRAPHY Mark Paul P. Pastrana‚ Mariah Ericka M. Patawaran‚ Princess Juneire M. Peligro‚ Francisco Q. Pua III‚ Rose Anne L. Quyo and Janille P. Ragpa Group 8 2B Medical Technology Organic Chemistry Laboratory ABSTRACT The main objectives were to separate the colored components of malunggay leaves by means of column chromatography‚ as well as to determine the purity of the components using thin layer chromatography (TLC) and measure the Rf values of the colored
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Introduction In order to understand what HPLC‚ that is‚ High Performance Chromatography‚ it is necessary to understand the meaning of the term chromatography itself. Chromatography Chromatography is defined as a procedure by which inert materials and drug principles encountered in pharmaceuticals preparations are separated by fractional extraction‚ adsorption‚ or ion exchange on a porous solid. Chromatography is particularly useful as a means of separating and purifying complex and closely
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Column and thin layer chromatography of plant pigments CHEMISTRY 201L EXPT 04 PAGE 01 - 12 Noel Angelo P. Kalacas*‚ Hanna Mae Laluces‚ Ina Bianca Lanuza Department of Chemistry‚ College of Science *Corresponding author; e-mail: knight_BeNcH66@yahoo.com Abstract Chromatography is a powerful technique for separating and/or identifying the components in a mixture. There are different types of chromatography and each has its own strengths and weaknesses. In this experiment‚ pigments of the
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Date Submitted: January 6‚ 2014 Experiment 4: Extraction and Chromatic Separation of Plant Pigments from Tomato Paste ABSTRACT In column chromatography a mixture is dissolved in a solvent and poured over a column of solid adsorbent then eluted with the same or a different solvent. This method is often used for preparative purposes; when a relatively large amount of the mixture and the components need to be isolated.
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Gas Chromatography (GC) Definition: Gas chromatography is a physical method of separation in which the components to be separated are distributed between two phases‚ one being a stationary bed of large surface area‚ and the other a gas that percolates through the stationary bed. When the stationary phase is a solid‚ the separation process is more precisely called gas-solid chromatography. Introduction: Gas Chromatography is a technique widely used to separate complex mixtures of substances. Compounds
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Column Chromatography ________________________________________________ You have already performed two chromatography experiments: gas chromatography and thin layer chromatography. All chromatography experiments involve passing a mixture of analytes through a system that includes a mobile phase and a stationary phase. The partitioning of the analytes between these two phases determines the rate at which they pass through the system‚ and (in theory) allows them to be separated from one another. Column
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Theoretical Ratio | Obs. Ratio | Obs.m.p. | Physical Appearance | Ethyl 4-aminobenzoate | .233g | .2093 | 89% | 30% | 20% | 89 ° C | Off white appearance; powdery | Benzoic Acid | .233g | .243 | 104% | 30% | 20% | 125 ° C | Clear crystals | 9-fluorenone | .311g | .623 | 200% | 40% | 60% | 82 ° C | Yellow‚ filmy‚flakes | Discussion: Based on the results‚ the most accurate extraction achieved was the first compound‚ ethyl 4-aminobenzoate. With an 89% actual yield of the compound‚ a 3rd or 4th
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