with rhamnose in a bacterial system suitable for the RNA T7 polymerase. The recombinant protein will be isolated‚ purified and characterized by SDS-PAGE and Western blot. Several chromatographic techniques including gel filtration and affinity chromatography will also be employed for the purification of the recombinant human PDGF-B. Final Report Submission BIND 5005-Fall 2009 INTRODUCTION. The PDGF family consists mainly of four isoforms called as PDGF-A‚ PDGF-B‚ PDGF-C and PDGF-D‚ where
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for H. sp. KHS3. To our knowledge‚ most of the studies on Halomonas sp. focused on degradation of monoaromatic rather than polycyclic and heterocyclic compounds. We have demonstrated that H. sp. KHS3 has the ability to growth using phenanthrene‚ fluorene and naphthalene as the only carbon source. Similar ability has been demonstrated by
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experiment we will learn microscale level lap techniques such as vacuum filtration‚ column chromatography‚ and centrifugation to extract the active ingredient from tablets. Separation and Purification Scheme: Analgesic with Binder Add methanol (CH3OH) dissolve analgesic Analgesic in solution with binder Centrifuge +filter some binder Analgesic in solution with binder Alumina column remaining binder Analgesic in solution with methanol Evaporate most
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ethanol‚ so the cooler surface of the sponge helped condense 1-butanol‚ but the ethanol still made it to the vial because of its lower boiling point. The sponge helped improve efficiency of the distillation. We will use distillation plot and gas chromatography to test it. To draw the distillation curve graph ‚ we counted the number of drops and temperature. The graph shows us the specific spots (large number drops) have a little change in temperature.it means that a compound has been distilled off
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Buckminster Fuller Contribution Disadvantages of Technique Define the following: The Cell: Optical tweezers: Fluorochrome: Electron Tomography: Centrifugation: Differential Centrifugation: Equilibrium Sedimentation: Homogenate: Electrophoresis: Column Chromatography: Isoelectric Focusing: Western Blotting: Flow Cytometry: 2-D surface metabolism: Tensegrity: Answers the following questions: What is the difference between Resolution and Magnification? What is the difference between Amplitude contrast
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source of antioxidant‚ has been demanded due to its biological and physiochemical properties. Therefore‚ there is a need to develop the form of Lycopene supplement. Lycopene was extracted from tomato wastes by using acetone and purified by column chromatography. The extract was characterized by UV-Vis spectrophotometer. An average recovery of 40 mg lycopene / kg tomato wastes was obtained. Lyco-cookies were prepared by using common ingredients containing 3 mg lycopene per serving. Introduction
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Final Organic Study Guide: Be able to develop an Experiment Beginning to End * Find a good reaction * Good reaction is defined by high yield‚ green chemistry (less waste‚ less harmful chemicals) * Reaction found on Reaxys * Find MSDS Sheet for all Compounds (Reactants‚ Products and Side Products) * It is important to know what you are working with * The toxicity category numbers are there to gauge how toxic the chemical is‚ 4 being most severe and 1 being least
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& RNA and fatty acids. • Spectroscopic Techniques: Structural determination of an Organic compound by using UV & Visible ‚ IR ‚ NMR ‚ MASS techniques. • Chromatographic Techniques: Paper‚ Column ‚ Gas-Liquid chromatography ‚ High performance liquid chromatography etc. Computational Skills: Operating Systems : Win XP‚ 2000‚ 98‚ Languages : C‚ C++‚ Packages : Ms-Office. Soft Skills: 1. Good
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Abstract In order to experiment with distinct components of a mixture‚ they must be first separated so they can be observed individually. This is accomplished in this lab by the technique called thin layer chromatography (TLC). TLC involves a stationary phase‚ which the TLC plates as well as a mobile phase‚ which could be one of two solvents used: ethanol-acetone for TLC. Dyes in a sample separate consequently because of their unique polarities. As a result‚ nonpolar substances travel further
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CONTENTS What is a Mixture? What are the types of Mixtures? Define:-solutions -suspensions -colloids (Draw and compare.) Methods of separation 1. Filtration 2. Distillation (simple and fractional) 3. Paper chromatography 4. Solvent extraction 5. Separating funnel 6. Evaporation 7. Crystallization 8. Sublimation Draw and explain each. What is a mixture??? A mixture is a combination of two or more substances that are not chemically united and
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