display similar characteristics. In this experiment‚ identification of the unknown ketone is accomplished through thin layer chromatography‚ melting point‚ and 1H-NMR spectroscopy. The unknown ketone is from a homologous series of methyl ketones. CH3CO (CH2) nCH3 The first step in the lab is the preparation of the solvent used in the developing chamber for thin layer chromatography. The solvent used is a 3:1 mixture of toluene and petroleum. After the developing chamber is prepared‚ it is essential to
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DEAE Sepharose fast flow anion exchange chromatography was used for partial purification of C. partellus gut proteases [Kumar‚ H.1997]. For the CPGPs‚ 50mM NaOH buffer‚ pH 9 was used to equilibrate the column. Gut homogenate equilibrated with the 50mM NaOH buffer‚ pH 9 buffer was loaded on the column and elution was carried out using a 0-0.6M NaCl gradient [Kumar‚ H.1997]. 2.0ml fractions were collected and analyzed for
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Term 1 - Summative Assessment Science (Theory) Question Paper Set - 1 Time: 3 to 3 ½ hours Max. Marks: 80 Instructions: 1. The question paper consists of two sections‚ A and B. You are to attempt both the sections. 2. All questions are compulsory. 3. There is no overall choice. However‚ internal choice has been provided in all the three questions of five marks each. You have to attempt only one option in each question. 4. All questions in Section A and all questions in Section B are to
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QUALITY EVALUATION OF PARACETAMOL IN THE BULK‚ DOSAGE FORMS AND BODY FLUIDS USING THE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) TECHNIQUE S. Asare-Nkansah and J.K. Kwakye Department of Pharmaceutical Chemistry‚ Faculty of Pharmacy and Pharmaceutical Sciences‚ Kwame Nkrumah University of Science and Technology‚ Kumasi‚ Ghana ABSTRACT High Performance Liquid Chromatography has been used to evolve an analytical procedure for the evaluation of the content of paracetamol in the bulk‚ dosage
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Have you ever heard about medical marijuana? Do you know how many people are using it to cure their illness and make their lives easier? Do you know that marijuana can stop cancer cells from spreading? The scientists found that there is a compound found in marijuana that can freeze the cancer cells and prevent them from spreading. Also‚ marijuana can be much more effective for medical purposes when eaten rather than smoked. Medical marijuana is usually used as a whole part of it. It means the part
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1) A peptide‚ KKDSRHSTTR is tightly bound to a negatively charged ion exchange column. The column is washed with a pH 7.2 HEPES buffer (50 mM) and the peptide does not elute. Suggest two ways that you can change the buffer that will make the peptide elute from the column. 2) What amino acid(s) is/are involved in crosslinking polypeptide chains. How can these crosslinks be cleaved and prevented from reforming? 3) A polypeptide is subjected to the following degradative techniques results
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Kayla Adkins Forensic Chemistry Exam 2 I have been honest and observed no dishonesty. 1. In specific detail‚ describe a published procedure for the definitive identification of Heroin utilizing gas chromatography-mass spectroscopy (GCMS). Heroin is a white crystalline powder with no odor and a bitter taste. It is a derivative of morphine in that the –OH (hydroxyl group) is replaced by an acetyl group‚ -OCOCH3 and has a molecular formula is C21H23NO5 with a molecular weight of 369.42
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Extraction Report For experience 4c we were supposed to determine which one is the organic layer in three different tubes that has two layers. To determine which is aqueous (water) or organic‚ I used the technique of adding droplets of water to each tube. If the layer is water‚ then the drops of added water will dissolve in the aqueous layer and increase it’s volume. If the added water form droplets or a new layer‚ then it is the organic layer. Tube 1 (water and n-butyl chloride)‚ after adding
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Student Number: xxx Class: xxx Aim The aim of this experiment was to determine the concentration of aspartame in a sample of diet Coke® using HPLC as investigation tool. Introduction High Performance Liquid Chromatography (HPLC) is one of the most widely used analytical techniques of nonvolatile organic compounds. Chromatographic processes can be described generally as mass-transfer between stationary and mobile phase. In HPLC‚ a liquid mobile phase is used to
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Results In this experiment we used paper chromatography to determine the amount of chlorophyll a‚ chlorophyll b‚ xanthophyll‚ and beta-carotene in a tube of blended leaf extract. We recorded our findings on the table labeled table 1. Table 1 shows the transmittance at each wavelength on a table from 400 to 720. The information on Figure 1 came from the leaf extract on the paper chromatography that we used; with the help of acetone we saw the spectrum and the differences of the different pigments
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