Lab #3: Ion Exchange Chromatography Objective The purpose of this experiment was to separate proteins on the basis of their net charge at a particular pH. In cation exchange chromatography positively charged molecules are attracted to a negatively charged column. Conversely‚ in anion exchange chromatography‚ negatively charged molecules are attracted to a positively charged column. Experimental results could be monitored in a predictable way by controlling running pH‚ salt concentration‚ and by
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________________________12345_______________________ Date ____23.8.2013___________ Block _______ Training Lab: Paper Chromatography I. Background Information: When working in a lab‚ scientists often need to identify different molecules that are present in a sample they are studying. There are many ways to identify unknown molecules/chemicals in a sample. The method you will be using today is called Paper Chromatography and consists of 2 steps. First‚ you will separate the unknown chemicals and then you will identify
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Dr. Pepper Snapple Group Case Study Financial Management Derks‚ V (3997979). Doyle‚ D (4137531) & Ichev‚ R (4111443). Commissioned by the University of Utrecht. Introduction Originating to as early as 1880‚ Dr Pepper has become one of the most famous producers of carbonated drinks around the world. On May 7‚ 2008 the brand was spun-off from its parent company‚ Cadbury Schweppes Americas Beverages‚ or CSAB. The company was split into two with Dr. Pepper Snapple Group controlling
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Thin-Layer Chromatography of Analgesics Abstract: In this experiment thin-layer chromatography (TLC) was used to determine the composition of an unknown mixture of analgesics given five references of AC‚ ASP‚ CAF‚ IBU‚ SAL‚ an unknown consisting of a mixture of three of the references‚ and two unknowns (6‚23) containing a single reference compound. The composition of the unknown mixture and two single unknowns were determined by spotting the reference compounds and the unknown compounds on two
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the right numbers for our calculations. Mixture is defined as a substance made by mixing other substances together‚ in this case we will be dealing with salt and sand of course to undergo the process of separation. Distillation‚ Filtration‚ and Chromatography are known alternatives to separate the
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Chromatography refers to a set of laboratory methods used in separating as well as purifying biomolecules. A variety of chromatography techniques exist‚ and all depend on the interaction between a stationary and a mobile state. Two types of chromatography methods were examined in this investigation. First‚ ion-exchange chromatography was used. This method separates ions and polar molecules based on their affinity to the ion exchanger [2]. Specifically‚ cation-exchange chromatography was performed
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Introduction The soft drink industry is one of great and increasing power. With powerhouse companies such as the Coca-Cola Company and Pepsi Co dominating the market‚ Dr. Pepper Snapple Group has remained a close competitor with their line of soft drinks. While the demand of carbonated beverages has consistently been decreasing over the past few years due to health concerns‚ the attraction to diet beverages has boosted due to the idea of it being a healthier alternative to full-calorie sodas. Perceived
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The Brazilian pepper tree‚ Schinus terebinthifolius‚ is an invasive plant species that was introduced to Florida from South America. This particular plant presents a serious hazard to the biodiversity of Florida’s ecosystems‚ as it tends to eliminate native plant species wherever it grows. Since the Brazilian pepper tree was brought over from South America in the mid-1800’s‚ the reach of the plant has swelled to over 700‚000 acres‚ creating poor habitats for Florida’s native species (Center for Aquatic
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Date:7.6.2013 Objectives: Part 1: (i)To learn the technique of TLC and the visualization of colourless components. (ii)Identify the unknown drug by a TCL comparison with standard components. Part2 :to learn the separation technique by using TLC plate in separating a mixture of compound by using spinach leaf. Apparatus:UV lamp‚capillary ‚250 ML beaker Materials:aspirin‚acetaminophen‚caffeine‚unknown A‚unknown B‚TLC plates‚ethyl acetate‚Hexane‚acetic acid‚iodine Introduction: Procedure:
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are the materials that they are going to use? Will it affect the growth of the plant in terms of its height‚ number of leaves‚ and number of fruits? Background of the Study Bell peppers and many hot peppers are native to America and Asia. It is always been one of the common vegetables n the home garden. Bell peppers need lots of nitrogen‚ calcium‚ and phosphorus in order to maintain its good quality. Egg shells can be easily gathered because we have a lot of poultry in our community. It contains
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