Mr. Sousa Organic Chemistry ACL 8 January 2015 Chromatography Chromatography is a physical method of separating substances based on their properties‚ by distributing their components between a mobile and stationary phase. Chromatography is useful for observing mixtures and solvents‚ since it can be used to determine the relative bond strength of various compounds‚ a substances phase‚ and it can also the identity of unknown substances. Chromatography allows for the separation of chemical mixtures
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The Red Hot Chili Peppers Nick Gregory General Purpose: To Inform Specific Purpose: To Inform the audience of the history and achievements of the Red Hot Chili Peppers Central Idea: There is more to The Red Hot Chili Peppers than just a few hit songs INTRODUCTION I. Attention Getter: The Red hot Chili Peppers are more than just your average band. Currently they have won six Grammy awards and sold over 55 million albums worldwide. II. Preview: Tonight I am going to explain briefly the history
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The goal of this lab was to create and purify DHFR from BL21 ecoli using column chromatography. To know if we isolate DHFR their will be one band at 53 KD or above 50 KD and in our desalted eluate page should have the highestOD₂₈₀ which translates to the highest enzyme activity .Column chromatography is a method used to purify individual chemical compounds from mixtures of compounds. The main advantage of column chromatography is the relatively low-cost and disposability of the stationary phase used
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The Red Hot Chili Peppers are an american rock band that started in los angeles california. There was originally lead singer Anthony Kiedis‚ guitarist Hillel Slovak‚ bassist Flea‚ and drummer Jack Irons‚ They all met in high school at Fairfax high school in los angeles. They were signed to enigma records in december 1983‚ but 2 weeks earlier Irons and slovak had signed with MCA with the band What is this so they quit Red Hot Chili Peppers to pursue things with What is this so flea and Kiedis recruited
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Chromatography – Analyzing Analgesics by TLC and Isolation of β-Carotene by Column Chromatography Introduction/Background: Flavonoids are an important group of additives that can be defined as pure substances either natural‚ extracted from raw materials or synthetic. Chromatography is the separation of two or more compounds or ions caused by their molecular interactions with two phases – one moving and one stationary (Weldegirma 2012). Three types of chromatography are used
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Paper chromatography is one of the easiest methods of chromatography. It is a method of planar chromatography (stationary phase is in form of a plane). Paper chromatography follows the basic principle of chromatography‚ which states that substances or components are distributed in between the stationary phase and the mobile phase. It is an analytical technique‚ where only a small amount of a sample is used for separating and identifying its components. Like any other method of chromatography‚ paper
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Chromatography: How can we separate a mixture? Purpose The chromatography lab is to understand how molecules with similar molecular properties can be separated with paper chromatography. These differences will be interpreted to see the distinction of separate chemical substances. Pre Lab Questions 1. Explain capillary action as it pertains to water and paper. Capillary action makes water draw up the paper. As paper absorbs water mixes with the solutions in the paper. 2. What is the
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provided for. Good luck!!! CHROMATOGRAPHY (By: Mayflor Markusic) KALIKASAN: Everyday Science in Action Volume 4 No. 1; pages 22-24 a) What is chromatography? 1 2 3 4 ____________________________________________________________ ________________ b) Give examples of mixtures which can be separated through chromatography. 4 5 6 7 ________________________________________ ______________________________________ c) How is chromatography done? 4 5 6 7 ________
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Imagine waiting hours for one of the best bands in the entire world. Waiting hours on end to get kicked out of the line for front row to The Red Hot Chili Peppers. Anthony Kiedis‚ Chad Smith‚ Josh Klinghoffer and fucking Flea. It’s the seventh of June and we are at Bonnaroo Music and Arts Festival in Manchester‚ Tennessee. I had planned for months and months ahead of time to wait in line all day to get in the pit or the “very front row area” for the concert. This was my first attempt to be in the
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Search the web. Some interesting sites are listed below. Note that some of these sites go into much more depth than is reasonable for this course. http://en.wikipedia.org/wiki/Chromatography http://ull.chemistry.uakron.edu/analytical/Chromatography/ http://orgchem.colorado.edu/hndbksupport/TLC/TLC.html this is for TLC – similar to paper http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/C/Chromatography_paper.html http://jchemed.chem.wisc.edu/JCESoft/Programs/CPL/Sample/modules/paprchrom/paprchromdesc
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