L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally Affinity Chromatography on a Cibacron blue Sepharose stationary
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Title: Candy Chromatography Background Information and Research: Paper Chromatogrpahy is a analytical method that is used for seperating and identifying mixtures of color. There are two stages‚ the stationary phase and the mobile phase. The first is for a solid and the second for a liquid or gas. -Reference http://www.chemguide.co.uk/analysis/chromatography/paper.html Purpose: To see how paper chromatography works. Materials: · Candy with a colored coating‚ like Skittles® or M&Ms® (4 different
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IIT (BHU)‚ VARANASI UNITED PHOSPHORUS LIMITED Summer Training Report Submitted by KORRAPATI KRISHNA TEJA 27-Jun-12 A report on the projects undertaken‚ implant training during 14.05.2012 to 28.06.2012. Acknowledgement I take immense pleasure in thanking Mr. Bhupen Dubey‚ Head‚ Integrated Business‚ UPL and beloved Mr. Kabir Sahni‚ Head Of Site‚ UPL-Unit-8‚ Jammu for helping me to carry out my summer intern in UPL Unit-8‚ Jammu. I wish to express my deep sense of gratitude to my
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Musnit ------------------------------------------------- Corporation Wear Check offers many services for predictive and preventive maintenance‚ including competitive pricing and short turnaround time. Analysis are carried out at state-of-the-art‚ custom built‚ automated laboratories with interpretation and reports being completed by qualified staff. Other services include: * * Telephonic and on-site support. * Advice including in-depth investigations and trouble shooting by
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In conclusion‚ the original sample was used to collect different amounts of fraction. The original sample was Spirulina‚ which was spun down in a centrifuge before using. This process helps to separate the proteins from the tissues. Table 1 shows the amount of fraction collected from each sample. Based off the collected fractions‚ there is a larger quantity of yellow fraction present than the blue fraction. The yellow fraction remained in the filter for a longer period of time than the blue fraction
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which is glycerol is filtered off by a separation process and an impure biodiesel will be obtained. The biodiesel obtained is still contained a little amount of glycerol and other impurities. Hence‚ to get pure biodiesel‚ high-performance liquid chromatography (HLPC) is used to further purify the biodiesel. The biodiesel yield can be calculated relative to the initial amount of Pongomia Pinnata oil by weight and the biodiesel purity is determined
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Analysis of Ethanol in Moonshine | Using the GC-MS | Jennifer Greene | ------------------------------------------------- 4 May 2012 ------------------------------------------------- 4 May 2012 Introduction: In this experiment gas chromatography is applied to separate the water-ethanol mixture. This method is often used in the determination of alcohol in blood or urine. One obvious application is when law enforcement agencies need to determine whether or not someone is inebriated. In
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system? The retention time of caffeine and benzoic are related due to the presence of a hydroxyl group. The hydroxyl group impart polarity and induce dipole-dipole forces between the benzoic acid and the column. Thus‚ benzoic acid‚ being polar‚ are retarded by the column and will come out of the column with a longer retention time. 4. What is the peak width of caffeine? Peak Width = Area of peakHeight of peak x 2 Peak width of caffeine at 75ppm: 2304120272024 x 2 = 16.94 Peak width of caffeine
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Anionic Exchange Ion Chromatography to Detect the Anions Concentrations in the water Samples Narendra Boppana‚ Nasser Ali‚ Martin Chavez‚ Sear Introduction: Ion Exchange Chromatography is a process where the ionic exchange occurs in between the stationary phase and the sample in the mobile phase‚ by using this ionic exchange phenomenon it is easy to separate the compounds. Ionic exchange depends on the electrical properties
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Title: Liquid Chromatography Author: Gloria Contreras Lab Partner: Jose Montanez Instructor: Teresa Potter Date Work Performed: January 13‚ 2015 Date Submitted: January 20‚ 2015 Abstract: In this lab‚ liquid chromatography is used to separate the Red 40 and Blue 1 dyes inside of grape flavored Kool-Aid. It was determined that the 5% isopropanol will remove the Red 40 dye from the stationary phase. The 28% isopropanol will remove the Blue 1 dye from the stationary phase. The more
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