Residual solvents Identification and control of the residual solvents (USP 32(467)‚ page 181 Determination by gas chromatography with mass detector and headspace sampler Conditions of the chromatograph Agilent 7890A (LCTG CGMS): * Column DB-624 30m 0.25 mm 1.4µ (CG-2) * Injector temperatura: 160ºC * Flux: 1.0 ml/min * Split: 5:1 * Carrier gas: Helium * Time per test: 30.6 min Conditions of the detector 5975C inert MSD * Data adquisition mode: Scan/SIM * Mass:
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Purpose The purpose of this experiment is to exemplify how differences in molecular weight allow separation of polymers from their monomers. Methods of dialysis and gel filtration chromatography will be used to separate a glucose monomer from a starch polymer. Colorimetric glucose oxidase assay will be used to monitor the presence of glucose and a colorimetric iodine assay will be used to monitor the presence of starch in prepared solutions
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An eco-column is used to model an ecosystem on a smaller scale. The purpose of an eco-column is to examine how an actual ecosystem would respond to different conditions. Eco-columns typically have three chambers: the aquatic chamber‚ decomposition chamber‚ and terrestrial chamber. The aquatic chamber contains water and an aquatic plant‚ and it is at the bottom of the eco-column. The decomposition chamber contains items that will work as decomposers and as a possible catalyst for plant growth. Examples
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his story on the Trajan’s Column‚ and to have the effect he wished to convey. The sculptor wanted to tell the story of the emperor Trajan to the people of Rome and illustrate his victories and achievement by the carvings on the column. He uses the technique “continuous narration” to let the story unfold within the spirals without any interruption. This progression of events is not always consecutive and is sometimes simultaneous. Due to the increasing distance of the column from the ground‚ the sculptor
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Column Writing * - is a kind of writing that is personal and biased. Usually being written by veteran journalists (or those “graduated” from beat reporting); editors (either news editor‚ sports editor‚ business editor or entertainment editor) and also journalists. * - it is a feature that appears each issue under the same head‚ usually in the same place and is written by the same person or persons on some timely subject. Functions/Characteristics FUNCTIONS: 1. Typically attempt to persuade
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Backround: Gas Chromatography separates organic samples much in the same way as column chromatography. The only differences are that it uses a moving gas phase and a stationary liquid phase‚ and that the temperature of the gas system can be controlled. In a gas chromatograph the sample is shot in with a syringe and is immediately vaporized in a heated injection chamber. It is then introduced to a moving stream of gas called the carrier gas which sweeps the vaporized sample into a column filled with
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Organic Chemistry Laboratory I Experiment #6 - Column and Thin-Layer Chromatography (TLC) of a Spinach Extract Pre-Lab Assignment (1) List 5 ways that TLC can be used in an organic chemistry lab experiment. To identify an unknown‚ to monitor the course of a reaction and assess the purity of its product‚ to determine the best solvent for a column chromatography separation‚ to determine the somposition of each fraction from a column chromatography separation‚ and to determine whether a substance
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Abstract In this experiment paper chromatography was used in order to identify two unknown amino acids using eight known amino ones. The two unknown ones were identified by comparing the distance they travelled up the chromatography paper and their Rf values to the corresponding values of the other eight known amino acids. The unknown amino acids identified were Glycine and Methionine. Introduction Proteins in cells are important in many ways. There are different types of proteins such as
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3.7 Nuclear Independent Chemical Shift Analysis The Nuclear Independent Chemical Shift is measured as magnetic shielding of a molecule have been calculated at the B3LYP/6-31G theory level. Aromaticities are represented by negative NICS Values and antiaromaticity is represented by positive NICS Values. The large negative NICS Values of the title compound represent stronger aromaticity [29-35].Our present investigation of the density functional calculations of doped fullerene with glycine shows several
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Objective: The goal of the experiment was to use UV/VIS spectroscopy to collect data to quantify acetaminophen‚ aspirin‚ and caffeine from a migraine tablet where there is heavy spectral interference using Target Factor Analysis1. The migraine tablet has other ingredients in it like FD&C blue #1‚ stearic acid‚ and saccharin. Saccharin is the ingredient thought to have the biggest interference and was used as the experimental basis2. Instruments: UV/VIS spectroscopy was used to analyze the absorption
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