Ink Chromatography Lab Background Information Paper chromatography is an analytical method that is used for separating and identifying mixtures of substances into their smaller parts. Paper chromatography works because the ink used contains several dyes (pigments) that when place on porous paper are dissolved in a solvent by capillary action. When the pigments (solutes) are dissolved in the solvent (water & alcohol mixture) they move through the paper at different rates depending on their
Premium Solubility Color Pigment
PAPER AND THIN-LAYER CHROMATOGRAPHY Lab 47 Michael Stravino‚ CHM 2211L‚ June 3‚ 2012 Abstract Thin-Layer Chromatography and Paper Chromatography can be used to identify identical compounds‚ unknown compounds‚ the number of components in a mixture‚ as well as other important uses not explored in this laboratory. This experiment explores these techniques to identify an unknown amino acid by TLC and analyze food colors using paper chromatography. By using 2.0 mL of dimethoxyethane and 18 mL of
Premium Chromatography Food coloring Thin layer chromatography
RESULTS AND DISCUSSION: Ultraviolet spectroscopy of Verapamil hydrochloride at λmax of 278 nm in pH 1.2 buffer was found to be linear in range of 20 – 80 µg/ ml. The method was validated and found to be precise‚ specific and accurate. The tablets of Verapamil hydrochloride were prepared by press coating technique showing sustained release for 24 h for once daily dosing with a lag phase of 4 - 6 h. The optimum ratios and type of polymers and other excipients were chosen to produce gastroretentive
Premium Chemistry Acetic acid Alcohol
Salary: $4977.10 - $6183.10 / mo Final Filling Date: Continuous Duties: The Criminalist is the entry‚ training and sub journey level for the series. Criminalist will perform routine and less complex technical laboratory analyses and assist higher-level Criminalist in the examination of crime scenes and in the scientific investigation of crimes. Requirements: Education: Equivalent to graduation from college with a major in one of the physical or biological sciences‚ including the equivalent of
Premium Scientific method Science Research
CH3I Before coming to lab‚ please review the following techniques: "Reluxing a reaction‚" "Extraction and washing‚" "Drying an Organic Solvent‚" "Evaporating an Organic Solvent‚" "Analyzing a Solution by Thin Layer Chromatography (TLC)‚" "Separating Mixtures by Column Chromatography‚" and "Characterizing Compounds by IR." Notice that this lab brings together most of the techniques that we have used this semester. Then complete the following table before coming to class so that you will be prepared
Free Chromatography Thin layer chromatography Distillation
Abstract Finding the melting point of an organic substance is a practical and efficient way for scientists to identify an unknown substance or determine a known substance’s level of purity. When organic substances are mixed together in varying degrees they take on a melting characteristic that is lower and broader than in its pure form. This property was manipulated in the lab to observe the various melting points of Naphthalene and Biphenyl when the percentage of composition was altered. A
Premium Liquid Temperature Solid
Figure 3.1: Typical standard chromatogram of Ritonavir and Lopinavir Figure 3.2: Typical sample chromatogram of Ritonavir and Lopinavir 3.3.2 Method validation The optimized RP-HPLC method for simultaneous assay of Ritonavir and Lopinavir was validated according to ICH guidelines with respect to system suitability‚ linearity‚ sensitivity‚ accuracy‚ precision and robustness. 3.3.3 System suitability Before sample analysis‚ the chromatographic parameters used in this analysis must conform the system
Premium Analytical chemistry Chromatography
Tiboelectric Series Lab Fleur Vande Kerckhove 9A Purpose/Aim: How do the triboelectric series from different materials classify themselves due to their affinity (gaining or losing) when being measured by a gold leaf electroscope? Hypothesis: If the materials change (from: wool‚ rabbit fur‚ cotton‚ aluminum‚ nylon‚ amber‚ celluloid‚ silver‚ silicon and acrylic) to classify their affinity of their triboelectric series then rabbit fur will have a higher affinity compared to the rest of the materials
Premium Oxygen Light Ultraviolet
Candy chromatography My experiment is called candy chromatography. This project is mainly about the colors that are mixed with other colors to make candy markers Koolaid and much more. I think that only the color of the candy is going to show up on the coffee paper. I think that threw the entire experiment the color in the candy is going to show up the entire time. I think this is going to happen because most of the colors are solid colors. The material and equipment used was a sample of candy
Premium Color White
Chromatography of Commercial Analgesics Chromatography of Commercial Analgesics CHEMISTRY 200L EXPT 4 PAGE 8 - 11 CHEMISTRY 200L EXPT 4 PAGE 8 - 11 Janna Vernice R. Villalon*‚ Christian V. Villanueva‚ Cyd Vincent L. Zamora Department of Chemistry‚ College of Science *Corresponding author; e-mail: janna.villalon@rocketmail.com Abstract In analyzing the chromatography of analgesics‚ thin layer chromatography (TLC) was used. A very thin (micron) film of silica is coated on a glass
Free Chromatography Thin layer chromatography Chemistry