Lab #1 Extraction and Thin Layer Chromatography Kaya Gaudet 6784928 January 9‚ 2013 Line Structure References BenzophenoneBiphenylBenzoic Acid Extraction: A process used to separate different compounds in a mixture based on their solubility in an immiscible substance. Usually an aqueous and organic phase are used. During extraction the desired compound moves one phase and leaves the unwanted substance behind. Extraction normally has to be done a few times to leave all of the impurities
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& RNA and fatty acids. • Spectroscopic Techniques: Structural determination of an Organic compound by using UV & Visible ‚ IR ‚ NMR ‚ MASS techniques. • Chromatographic Techniques: Paper‚ Column ‚ Gas-Liquid chromatography ‚ High performance liquid chromatography etc. Computational Skills: Operating Systems : Win XP‚ 2000‚ 98‚ Languages : C‚ C++‚ Packages : Ms-Office. Soft Skills: 1. Good
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velmpharm24@gmail.com A precise‚ accurate and rapid RP-HPLC method has been developed and validated for the simultaneous estimation of Tamsulosin Hydrochloride and Finasteride in tablet dosage form. Chromatography was carried on RP – 18e Hiber RT (250 × 4.6) column using Water:Methanol (30:70%) v/v as mobile phase at a flow rate 0.7 ml/min and the effluent was monitored at 225 nm. The retention times of Tamsulosin Hydrochloride and Finasteride were 5.4 and 15.4 minutes respectively
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Briefing Thin-layer chromatography (TLC) is an extremely valuable analytical technique. It provides a rapid separation of compounds‚ and gives an indication of the number and nature of the components of a mixture. TLC can also be used to identify compounds by comparison with known samples‚ to check the purity of a compound‚ or to monitor the progress of a reaction‚ an extraction‚ or a purification procedure. This experiment will introduce you to the mechanics of TLC‚ and the chemical principles
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Spinal cord injury happens when an acute traumatic damage to the a certain area or the complete spinal cord (including the intervertebral disc joints) or nerves roots within the spinal column occurs. This type of injury can cause stretching‚ bruising‚ pressure‚ severing and laceration. The cauda equina is a bundle of spinal nerves and spinal nerve roots‚ that can be damaged as well and results in temporary or permanent loss of movement
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Stenosis means "closing in" or "choking". Spinal stenosis describes a condition in which the nerves in the spinal canal are closed in‚ or compressed. The spinal canal is the hollow vertical hole formed by the bones of the spinal column. Anything that causes this bony hole to shrink can squeeze the nerves inside. This narrowing can irritate the nerve roots that branch out from the spinal cord‚ or it can squeeze and irritate the spinal cord itself. This may cause pain‚ numbness‚ or weakness‚ most
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solid phase extraction cartridges (PGC)‚ e.g. Thermo Hypersil keystone Hypersep Hypercarb 200 mg‚ 3 ml cartridges‚ from Thermo Hypersil Keystone‚ UK (b) C18 solid phase extraction cartridges‚ e.g. Isolute C18(EC) solid phase extraction columns‚ size 1 g sorbent in 6 ml reservoir from Argonaut Technologies‚ Mid Glamorgan‚ UK (c) Glass-fibre filters (e.g. Whatman GF/C)‚ diameter 25-70mm (d) Argon or nitrogen‚ >99.99% (e) Borosilicate test tubes or vials‚ >3 ml capacity
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January 27‚ 2014 Introduction This laboratory has its first objective to familiarize with the thin layer chromatography (TLC) technique. Second‚ each student has to identify the unknown components by comparing it to one of the tree dissolved liquid analgesics: Acetaminophen‚ Aspirin and Caffeine. Finally‚ calculating the Rf value by measuring the mobile and stationary phase. Chromatography is a technique used to separate mixtures into their compounds. It’s based on a mobile phase and a stationary
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Scoliosis is one of three main types of spine curvature disorders. Like a disease on the spinal curvature twisting and resulting in the sideway curves of the spine. It is defined as a lateral‚ or a side-to-side deviation from the normal frontal axis of the body. Scoliosis is similar to Lordosis‚ which also been named the swayback‚ for example when the spine of a person with lordosis curves significantly inward at the lower back. Kyphosis another characterized by an abnormally rounded upper back (more
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Proteins (ACTIVITY 8) Gallemit‚ Lacei Machelle• • January 17‚ 2013 ABSTRACT Chromatography is one of the most useful methods of separating minute amounts of complex mixtures into the pure substances contained therein. Chromatographic separations are effected by allowing the components in the mixture to be distributed in two phases‚ one being stationary‚ and the other‚ mobile. Partition chromatography involves two main distribution systems‚ one system is composed of a gas and a liquid and
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