technique of TLC and the visualization of colourless components. (ii)Identify the unknown drug by a TCL comparison with standard components. Part2 :to learn the separation technique by using TLC plate in separating a mixture of compound by using spinach leaf. Apparatus:UV lamp‚capillary ‚250 ML beaker Materials:aspirin‚acetaminophen‚caffeine‚unknown A‚unknown B‚TLC plates‚ethyl acetate‚Hexane‚acetic acid‚iodine Introduction: Procedure: A.spotting of the TLC plates 1.A TLC plates is obtained
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be fully grown and I can’t wait to see what they taste like fresh from the soil. I had better go and water them now . . . RADISH EXTRACT Making use of radish extract as an effective alternative method of getting rid of insects and pests like worms‚ ants‚ and aphids was studied in this research project. The purpose of this experiment is to prove that radish extract can be used as an insecticide. The researchers first gathered all the materials needed to complete the experimentation. First
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Plant Pigment Chromatography VII. Analysis (Questions) 1. What factors are involved in the separation of pigments? Factors that are involved in the separation of pigments can include pigment solubility‚ the attraction between the pigments and paper and the size of each pigment particle. Because of these factors the results were as they were. Beta-carotene traveled the furthest because it forms no hydrogen bonds to the chromatography paper and is slightly soluble in the solvent. Contrastingly
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following questions: 1. Can radish extract be used as a pesticide? 2. Can this be as effective as the other commercially available products with the same purpose? 3. Can this be harmless to our health? 4. Can this be cheaper compared to other commercially available products with the same purpose? C. Hypotheses 1. Radish extract pesticide cannot be used as a pesticide. 2. Radish extract pesticide is not as effective as the
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Component seeks to separate‚ identify‚ quantify and analyze the active component found in Biogesic®‚ which may be useful for public awareness despite its wide acceptance and availability in the market. Furthermore‚ this study applies thin layer chromatography (TLC) technique since it is the simplest‚ cheapest yet most effective approach in terms to the study’s objectives. The TLC method implies Rf (Rate of flow) values where it is given by the distance from origin to center of subtance spot over the
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Data and Conclusions: The purpose of this experiment was to learn how to use distillation and gas chromatography to separate and identify different compounds from a given mixture. There are several kinds of distillation methods. However‚ the method that we used in this experiment was fractional distillation. This method is used when trying to separate two different volatile compounds whose boiling points differ by 40-50°C or more. If the boiling points are too close‚ this method
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Chromatography • • • Separation based on polarity of compounds Two potential phases for a compound to exist in: mobile and stationary Partitioning of compounds between mobile phase and stationary phase occurs: o Compounds that are less polar move more in the mobile phase‚ those that are more polar “stick” more on the stationary phase o These polarity differences cause compounds move at different rates and therefore can be separated 1. Mobile Phase: the phase the moves; can be gas or
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Gas chromatography (GC) is a chromatography technique where the separation of individual components (analytes) from a sample relies on their differing distribution between a mobile and stationary phase. The mobile phase carries the analytes through the stationary phase. In GC‚ it’s an inert gas (usually helium or nitrogen). The gas must be inert‚ so it won’t react with the sample to give a false reading. The stationary phase is a substance fixed in place to which the sample adsorbs because
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of Chlorophyll from Fresh Spinach and Investigation of the Photochemistry of Chlorophyll Chlorophyll a Chlorophyll b E5 - Extraction of Chlorophyll from Fresh Spinach E6 - Investigation of the Photochemistry of Chlorophyll The aim of this experiment is to investigate the photochemistry of chlorophyll. This experiment will be performed in two lab periods. In the first lab period you will extract chlorophyll‚ the green pigment in leaves‚ from spinach. In the second lab period
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Review Bubble column reactors Nigar Kantarcia‚ Fahir Borakb‚ Kutlu O. Ulgena‚* aDepartment of Chemical Engineering‚ Bog˘azic¸i University‚ 34342 Bebek-Istanbul‚ Turkey bDepartment of Chemical Engineering‚ Yeditepe University‚ 34755 Kadikoy-Istanbul‚ Turkey Received 31 August 2004; accepted 26 October 2004 Abstract Bubble columns are intensively used as multiphase contactors and reactors in chemical‚ biochemical and petrochemical industries. They provide several advantages during operation
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