Experiment 4 – Liquid Phase Chromatography I. Objectives This experiment’s goal is to explore one-dimensional and two-dimensional paper chromatography. II. Schematic Diagram of the Procedure PAPER CHROMATOGRAPHY Wash leaves‚ cut them into smaller pieces; in a mortar macerate them in circular motion Add 8mL ethyl alcohol to extract pigments‚ continue macerating until finely grounded Transfer extract to evaporating dish‚ allow to conc‚ don’t let extract to dry out Concentration
Premium Central nervous system Nervous system Water
experiment is to identify an unknown proprietary drug using thin-layer chromatography. The unknown’s behavior in thin-layer chromatography will be compared with that of its possible component analgesics. The possible unknowns and their analgesic ingredients will be Anacin (aspirin‚ caffeine)‚ Excedrin (acetaminophen‚ caffeine‚ aspirin)‚ Motrin (ibuprofen)‚ and Tylenol (acetaminophen). Introduction Thin-layer Chromatography(TLC) was the method used to figure out and identify the drug. TLC is done
Premium
their mouths and a happy‚ satisfied grin on their faces. The reason for conducting the research is to know how the colors of our favorite candy-coated sugary rewards came to be and that method of knowing is what we call “Candy Crhomatography”. Chromatography by definition according to Webster’s dictionary means “ a method for separating the constituents of a solution (gas or liquid) by exploiting the different bonding properties of different molecules”. In this case‚ the molecules being broken down
Premium Thin layer chromatography Color Scientific method
the Kool-Aid was correct. However‚ solely based upon the Rf values‚ the dyes in the green Kool-Aid are Red 40 and Yellow 6 as those are closet Rf value to the numeric data collected and calculated from the Kool-Aid chromatogram. However‚ the chromatography paper in both trials display that the dyes in Kool-Aid are a form of yellow and a form of blue because one color band was of a blue tint and the other‚ a yellow tint. Therefore‚ based on this qualitative data‚ the dyes in the green Kool-Aid are
Premium Food coloring Dye Color
Information and Research 1. Chromatography is an analytical methor or technique that serves mainly as a tool for the examination‚ separating and identifying mixtures of chemical substances that are or can be coloured. 2.check the presence of any contamination in the manufactured compounds h as medicine‚ Contaminants in rainwater Analysis of narcotics Detection of substances in urine http://en.wikipedia.org/wiki/Paper_chromatography http://www.chemguide.co.uk/analysis/chromatography/paper.html http://chem
Premium Chromatography Analytical chemistry Gas chromatography
Separations: Chromatography of M&M and Ink Dyes Almost all substances we come into contact with on a daily basis are impure; that is‚ they are mixtures. Similarly‚ compounds synthesized in the chemical laboratory are rarely produced pure. As a result‚ a major focus of research in chemistry is designing methods of separating and identifying components of mixtures. Many separation methods rely on physical differences between the components of a mixture. For example‚ filtration takes advantage of substances
Free Chromatography Thin layer chromatography
BSC 2010L November 4th‚ 2015 Effects of Light Intensity and Wavelength on Photosynthesis of Spinach Leaves By replacing the air pockets in spinach leaf disks with a bicarbonate solution and allowing the disks to sit in the solution while being exposed to different light conditions‚ we were able to observe the effects of various light intensities as well as colors on the rate of photosynthesis. As the spinach leaves photosynthesized‚ oxygen pushed out the solution and filled the intercellular spaces
Premium Photosynthesis Light Chlorophyll
Lab #3: Ion Exchange Chromatography Objective The purpose of this experiment was to separate proteins on the basis of their net charge at a particular pH. In cation exchange chromatography positively charged molecules are attracted to a negatively charged column. Conversely‚ in anion exchange chromatography‚ negatively charged molecules are attracted to a positively charged column. Experimental results could be monitored in a predictable way by controlling running pH‚ salt concentration‚ and by
Premium Electric charge Electron
Chromatography: Separating Mixtures Introduction: Magic marker inks are often mixtures of several compounds. Paper chromatography is a common method of separating various components of a mixture. After separation‚ you can observe the different colors that make up a particular color of magic marker ink. You can also calculate a ratio Rf‚ which compares how far each compound traveled to how far each solvent (substance that dissolves another substance) traveled during the experiment.
Premium Mixture Chemical substance Chemical compound
Thin-Layer Chromatography of Analgesics Abstract: In this experiment thin-layer chromatography (TLC) was used to determine the composition of an unknown mixture of analgesics given five references of AC‚ ASP‚ CAF‚ IBU‚ SAL‚ an unknown consisting of a mixture of three of the references‚ and two unknowns (6‚23) containing a single reference compound. The composition of the unknown mixture and two single unknowns were determined by spotting the reference compounds and the unknown compounds on two
Premium Thin layer chromatography Acetic acid