the right numbers for our calculations. Mixture is defined as a substance made by mixing other substances together‚ in this case we will be dealing with salt and sand of course to undergo the process of separation. Distillation‚ Filtration‚ and Chromatography are known alternatives to separate the
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Chromatography refers to a set of laboratory methods used in separating as well as purifying biomolecules. A variety of chromatography techniques exist‚ and all depend on the interaction between a stationary and a mobile state. Two types of chromatography methods were examined in this investigation. First‚ ion-exchange chromatography was used. This method separates ions and polar molecules based on their affinity to the ion exchanger [2]. Specifically‚ cation-exchange chromatography was performed
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possible to identify which peak correlates with which compound. Figure 2 contains a peak at around 500 and 700 nm which is a good indicator that that would-be chlorophyll b. This makes sense because this was the bottom band from the thin layer chromatography and chlorophyll b is the most polar compound. Chlorophyll B contains an aldehyde where chlorophyll A has a methyl group making it slightly more polar. Following that‚ figure 4 contains
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Thin Layer Chromatography of Unknown Samples and Paprika By: Uyen Huynh Date: June 18‚ 2014 Lab Partners: Philip Murray Professor and section: M. Pandey‚ CHM2210L.601 Data: Analysis of Drug Store Items Knowns Rf Values Aspirin .91 cm Acetaminophen .85 cm Ascorbic Acid .86 cm Unknown .97 cm Identity of unknown mixture Acetaminophen Analysis of Paprika Pigments Spot Number Rf Color 1 1 Red-orange 2 1 Red-orange Calculations
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in the BIOL 1F90 Laboratory Manual (Martin‚ 2013)‚ Experiment # 1‚ pages 2-5. Results: Table 1: Culture Sample Chromatography Rf Values Sample of Spot Distance from Origin (mm) Solvent Front (mm) Rf Value Color A 6.0 57.0 0.22 Bluish green B 17.5 56.0 0.105 Dark green blue C 4.0 57.0 0.070 Greenish blue D 4.0 47.0 0.085 Green E 10.5 56.5 0.186 Light blue Figure 1: Chromatography- TLC sheet From left to right- acetic acid‚ citric acid‚
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Angela’s Ashes is an illuminating story written from a child’s perspective. The language of the book is simple‚ and the author recalls the memories of his youth with ease. The story mixes humor with the reality of poverty and depression. With McCourt’s dialogue‚ he effortlessly speaks with an “innocent eye”… Dialogue‚ innocent eye‚ dialect‚ use of quotations‚ comic relief. Intro here! The use of dialogue throughout Angela’s Ashes is well-chosen. When McCourt is in his younger years he uses a language
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Modern Family: Stereotype Central Modern Family is a show full of different stereotypes. The first or "pilot" episode of Modern Family showed a lot of strong evidence towards the show challenging dominant ideology. By representing the stereotype of the gay marriage between Cameron and Mitchell‚ this show successfully challenges the dominant ideology of a straight marriage‚ in which the child has both a mother and a father‚ and not just two fathers. When the audience sees this‚ they now see that
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amylose affinity chromatography was performed. The amylose resin present in 20% ethanol was first diluted by adding 10 mL of 20 mM TrisHCl‚ pH 7.4‚ 0.2 M NaCl‚ 1 mM EDTA and centrifuged at 700 rpm for 5 minutes. After decanting the buffer‚ another 10 mL of TrisHCl‚ pH 7.4‚ 0.2 M NaCl‚ 1 mM EDTA was added to this resin solution and centrifuged at 700 rpm for another 5 minutes to further dilute the ethanol concentration in the resin. After the buffer was decanted again‚ the column was packed using
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ABSTRACT The study on the production of chalk out of sea shells was designed to producequality dustless and long lasting chalk to compare with the standard existingcommercialized chalks if there are any‚ which was sold in the market. The chalk out of sea shells is for local or non-local used for schools or any educational aspects or even athome which can be formed into different sizes and colors according to your will. We‚ theresearchers‚ used the sea shells to recycle and transformed it into a
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Trajan’s Column with the Bayeux Tapestry Name Institution Historical narratives have recurrently been found in art across different cultures. This has been done using a variety of media and through the utilization of diverse methods that establish chronology. This use of different forms of media and methods has also been utilized to demonstrate the relationship between separate events. Some historical narratives such as the ones represented in the Trajans Column and the Bayeux Tapestry are continuous
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