Objectives: To use the process of chromatography to separate plant pigments. To compare the plant pigments found in spinach and red leaf lettuce leaves. Hypotheses: I believe the spinach leaf in the acetone will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. I believe the spinach leaf in distilled water will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. Discussion Questions:
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favorite candies? Chromatography can solve that for you. Chromatography is a separation technique used by scientists for separating both organic and inorganic compounds. There are four different types of chromatography: thin layer‚ liquid‚ gas‚ and paper‚ but for this lab paper chromatography will be used. Who invented chromatography? A Russian botanist named Mikhail Semyonovich Tsvet invented chromatography in 1901 while doing research on plant pigments. Why is chromatography so important? This
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Analysis of Food Colorings by Paper Chromatography Introduction Paper Chromatography one method for testing the purity of compounds and identifying substances. This is a useful technique because it is relatively quick and requires small quantities of material. Separations in paper chromatography involve the same principles as those in thin layer chromatography. The substances are distributed between a stationary phase and a mobile phase. The stationary phase is usually
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Candy Chromatography Background info: Paper chromatography is a logical technique used to separate works of a solution. Three examples of how we apply this technique to real-life would be: contaneminants in water‚ separation of plant pigmentation‚ and analysis of narcotics. Source: http://www.discoveriesinmedicine.com/Bar-Cod/Chromatography.html#b Purpose: To find out why candies are different colors. * Materials: Candy with a colored coating‚ like Skittles® or M&Ms® (4 different
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Column and Thin Layer Chromatography Beverly Abstract: Plant pigments were separated and concentrated from a crude spinach extract through the use of column chromatography and an eluatropic series of hexanes‚ hexane/acetone‚ and methanol. The pigments were analyzed using thin layer chromatography with a 30% ethyl acetate/hexane developing solvent. Introduction: Chromatography is a technique used to separate a mixture of two or more components based on
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CO2 on the rate of Photosynthesis in Spinach Leaves Introduction Photosynthesis is the process by which green plants and some other organisms use sunlight to synthesize foods from carbon dioxide and water. Photosynthesis in plants generally involves the green pigment chlorophyll and generates oxygen as a byproduct. The purpose of this experiment is to see how different concentrations of carbon dioxide affect the rate of photosynthesis in spinach leaves. I hypothesize that the greater
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ABSTRACT Separation and identification of components of a mixture was determined by using Chromatography. The components of a mixture have different affinities for a stationary phase as well as different affinities for a mobile phase. INTRODUCTION An extremely common technique by using two chemicals and filter paper can give you sufficient information. A well-done Chromatography of dyes will provide you with enough data to determine if a liquid mixture is heterogeneous or homogenous . In a learning
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Marketing II – Group Assignment Waters Chromatography Division: US field Sales Submitted by: Group 5‚Section D ArkayanBagchi (2010PGP063) Harveer Singh (2010PGP449) Kaushlendra Singh (2010PGP150) Krishna Kishore Burugula (2010PGP163) Loveneesh Solanki (2010PGP170) RohanWagh (2010PGP311) Sameer Morey (2010PGP325) Protagonist The cases does not give any specific issues to be solved by any
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CHROMATOGRAPHY OF PLANT PIGMENTS Marquez‚ Ma. Rica Paulene‚ Moises‚ Patrisha Kate‚ Policarpio‚ Jairus Paolo‚ Rolda‚ Zylene Joy Department of Biology‚ College of Science‚ University of the Philippines Baguio April 23‚ 2013 ABSTRACT The objective of this experiment was to apply the technique of paper chromatography as a method for separating individual plant pigments contained in plant tissue extracts containing pigment blends. The process of chromatography separates molecules because of the
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Liquid Chromatography – Laboratory #18 Introduction: We are using liquid chromatography to separate the colored substances in grape-flavored drinks. We separate the component dyes‚ and then we separate the flavorings and citric acids. Background: Chromatography is a process that is used to separate a substance into its component parts. The separation occurs between the stationary and moving phase of the lab. The moving phase consists of a fluid and the stationary phase consists of a solid
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