Questions 1. Why must you use lead pencil‚ instead of a pen‚ to mark your chromatography paper? A lead pencil should be used instead of a pen because the ink of the pen would dissolve in the eluting solution and separate. The components of the ink could mix with the other ink/ dyes that would be tested on the same chromatogram. Thus‚ a lead pencil should be used. 2. Why should you avoid touching the surface of the paper to be used for amino-acid chromatography? We should avoid touching the
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Discussion: My results show the following: The unknown component traveled at a distance similar to the spice time blue dye. However‚ there are a few limitations within this experiment. The accuracy of the length of the component was difficult to record. The ruler that we used was degraded and thick‚ making it hard to read the length. The dyes could also cause error to occur. Everything has an expiration date‚ depending on how old or how new the dye is‚ it could affect the outcome. 1. Why is it
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Micro-extraction of urine and/or stomach contents and analysis by TLC Abstract Thin Layer Chromatography (TLC) is one of the oldest chromatographic techniques used for the identification of compounds and for determination of the presence of trace impurities. The aim of the experiment was to determine two unknown samples (X and Y) by comparing results to the known standard drugs‚ of which there was five‚ using a specific TLC method. The same technique was used for the determination of
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Introduction Paper chromatography is a method using a chromatography paper to identify the many mixtures of one simple color. The word chromatography comes from the Greek words‚ "chroma‚" meaning color‚ and "grafein‚" meaning to write. The process of this experiment is to place a colored dot‚ such as marker ink‚ onto the chromatography paper‚ one centimeter away from the triangular tip. Next dip your chromatography paper into the solvent‚ not allowing it to touch the colored dot. Let it sit for
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Paper chromatography Presented by – Miss. Shruti Vilas Kharat. SYBSc / FS13164 History- Paper Chromatography (PC) was first introduced by German scientist Christian Friedrich Schonbein (1865). PC is considered to be the simplest and most widely used of the chromatographic techniques because of its applicability to isolation‚ identification and quantitative determination of organic and inorganic compounds. Definition- Paper chromatography is an analytical method technique for separating and
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Serratiopeptidase drug was purchased from MARS Therapeutics & Chemicals limited‚ Hyderabad‚ India. The standard drug Fenofibrate was purchased from USV limited‚ Himachal Pradesh‚ India. Assay kits for serum Total Cholesterol (TC)‚ Triglycerides (TGL)‚ High density lipoproteins were purchased from Erba Mannheim‚ Transasia Bio-Medicals limited‚ Himachal Pradesh‚ India. All other chemicals were of analytical grade. The experiment was conducted using [12] male Albino Wistar rats (150-200g)‚ at about
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Separation and Identification of Plant Pigments by Paper Chromatography Biology 1107L Introduction All living organisms require energy for their chemical processes. The ultimate source of this energy is the sun. Plants convert light energy into the chemical energy of sugars. During photosynthesis pigments are used to capture light energy. Pigments of green plants can easily be separated and identified using a technique called paper chromatography. The purpose of this
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Chemistry 100 Experiment #2: Chromatography of Food Dyes Name: Cassandra DeVaux Date: April 17‚ 2014 Purpose: To learn how mixtures of compounds can be separated and what food dyes are found in certain foods Introduction: In this exercise we will separate food dyes from a variety of sources. We will first gather data on known dyes where we know how many substances make up the dye. Then we will collect data on some samples where we do not know the make-up. We will compare our
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When people hear candy chromatography‚ they don’t know what to think about it. They of course know that it had something to do with candy. But just don’t know what. Candy chromatography is fun and simple. Candy Chromatography is a fun project‚ plus it has to do with candy. Candy Chromatography is an experiment to find out the true colors of the dyes in candy. When you put a skittle or an M&M in your hand in hot weather‚ the candy tends to bleed the color. And sometimes it shows more than one color
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Plant Pigment Lab Question: What pigments are present in Red Wandering Jews? Background: A leaf is filled with many pigments. The pigments are usually masked by the prevalence of the green chlorophyll. Anothocyanin(red or purple)‚ carotene(orange)‚ and xanthrophyll(yellow) are found in different proportions in different leaves. Leaf pigments can be separated by using paper chromatography. Paper chromatography is a technique that extracts pigments into a paper filter called chromatogram. What are
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