PAPER AND THIN-LAYER CHROMATOGRAPHY Lab 47 Michael Stravino‚ CHM 2211L‚ June 3‚ 2012 Abstract Thin-Layer Chromatography and Paper Chromatography can be used to identify identical compounds‚ unknown compounds‚ the number of components in a mixture‚ as well as other important uses not explored in this laboratory. This experiment explores these techniques to identify an unknown amino acid by TLC and analyze food colors using paper chromatography. By using 2.0 mL of dimethoxyethane and 18 mL of
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Isolation of β-Carotene by Column Chromatography Introduction/Background: Flavonoids are an important group of additives that can be defined as pure substances either natural‚ extracted from raw materials or synthetic. Chromatography is the separation of two or more compounds or ions caused by their molecular interactions with two phases – one moving and one stationary (Weldegirma 2012). Three types of chromatography are used in organic chemistry: Thin Layer Chromatography (TLC)
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the pure crystal constituents of the compound. Thin layer chromatography can be used as a physical method to segregate compounds from natural sources. E.g. Spinach leaves are visibly green‚ but consist of a variety of components that have more colour than others. This experimental procedure uses compounds from spinach leaves that are exposed to chromatography‚ TLC plate to indicate the different pigments within the spinach extract. ‘Column chromatography in chemistry is a method used to purify
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building blocks of other biomolecules like proteins and ezymes (Davidson‚ 2015). This experiment examined a mixture of 3 amino acids. The purpose of this experiment was to identify amino acids in a mixture through cation exchange chromatography and thin layer chromatography. This was done by examining the amino acids elution order from a cation exchange resin based on their isoelectric points and their different
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223 - Organic Chemistry Laboratory I Experiment #6 - Column and Thin-Layer Chromatography (TLC) of a Spinach Extract Pre-Lab Assignment (1) List 5 ways that TLC can be used in an organic chemistry lab experiment. To identify an unknown‚ to monitor the course of a reaction and assess the purity of its product‚ to determine the best solvent for a column chromatography separation‚ to determine the somposition of each fraction from a column chromatography separation‚ and to determine whether a substance
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Villalon*‚ Christian V. Villanueva‚ Cyd Vincent L. Zamora Department of Chemistry‚ College of Science *Corresponding author; e-mail: janna.villalon@rocketmail.com Abstract In analyzing the chromatography of analgesics‚ thin layer chromatography (TLC) was used. A very thin (micron) film of silica is coated on a glass plate or a sheet of aluminum or plastic. The ingredients of commercial analgesics were characterized in comparison with chromatograms of aspirin‚ Acetaminophen‚ caffeine and mefenamic
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khromatos (color) and graphein (to write); i.e. paper chromatography is literally color writing on paper. This method tests the purity of compounds and identifies substances. This analytical process‚ despite having been replaced by the success of thin layer chromatography‚ still stands as a valuable teaching tool‚ and is nevertheless very common. This method is very useful because not only is it a relatively quick process‚ but also requires only small quantities of materials. The substances being
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the chlorophyll and carotenoid pigments by using acetone as the solvent. The chlorophyll and carotenoid pigments were extracted by using column chromography and alumina was used as the solvent. Solvents of different polarities were used‚ starting with the least polar‚ to extract the certain components from the leaves. They were then analyzed by using thin- layer chromatography. Procedure: The first part of the experiment dealt with breaking down the spinach leaves in a mortar and pestle. Acetone
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Abstract In order to experiment with distinct components of a mixture‚ they must be first separated so they can be observed individually. This is accomplished in this lab by the technique called thin layer chromatography (TLC). TLC involves a stationary phase‚ which the TLC plates as well as a mobile phase‚ which could be one of two solvents used: ethanol-acetone for TLC. Dyes in a sample separate consequently because of their unique polarities. As a result‚ nonpolar substances travel further
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chlorophyll and beta carotene from plant leaves using column chromatography. 2. To qualitatively characterise the pigments with UV-vis spectroscopy and TLC. 2. Results and discussion Isolation of beta carotene and chlorophylls by column chromatography Upon the loading of S1 (the extract of the plant leaves in hexane)‚ a yellow band appeared at the top part of the silica column immediately after the solvent level descended to just above the sand layer. This yellow band later developed to about 2 centimetres
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