Unit 1: fundamentals of science. Title An investigation of an onion cell using a light microscope. Aim: The aim of this investigation is to identify the cells within an onion skin using a light microscope. An onion cell is a plant cell which through the light microscope‚ it should outline the cell wall‚ cell membrane and the nucleus. Introduction: Plant cells have a cell wall and cell membrane which animal cells do not have. The cell wall provides support and is relatively rigid‚ which consists
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Historical development of the cell theory‚ in particular‚ the contributions of Robert Hooke and Robert Brown. • 1665 English scientist Robert Hooke used microscope to examine thin slices of cork and saw small box-like compartments he called cells. He was first to realise plant material had organised structure at microscopic level. (compound microscope) • 1831 Scottish Botanist Robert Brown was involved in a dispute about how pollination and fertilisation occurred in plants. During his study with orchids
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Lab #1 notes: The Discovery Process Types of microscopes: Dissecting: has 2 eyepieces and used for looking at a fly. Is 3D and used reflecting light Lower magnification‚ higher resolution Compound: has 1 eyepiece and is used for looking at a cross-section of a plant. 2D and uses transmitted light Higher magnification‚ lower resolution Microscope objectives include 4x‚ 10x‚ 40x‚ 100x‚ -As you move to a higher power‚ your field of view decreases -Parcentric: whatever is in the middle of
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To prepare a temporary mount of aleaf peel to show stomata. B) Materials required:- Fresh leaves of plant‚ compound microscope‚ glass slides‚ cover slips‚ water‚ glycerine‚ safranine‚ blotting paper‚ needles‚ brush etc. C) Theory :- i) Stomata are minutepore present on the surface of the leaves. ii) Though they are found on both the upper and lower epidermis of the leaf‚ they are more in number on the lower epidermis. iii) Each stoma has two bean shaped
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intentionally blank 27 Laboratory 2: Scientific Methodology & Enzyme Activity 28 In-Lab Report Sheet 28 Objectives 33 Introduction 33 Parts of the Swift M5 Microscope 33 Part A: Using the Swift M5 Microscope and Viewing a Letter “e” 34 Lab 3: Microscopy 39 In-Lab Report Sheet 39 Part A: Using the Swift M5 Microscope and Viewing a Letter “e” 39 Laboratory 4: Cell Structure & Function 43 Pre-Lab Assignment 43 This page left intentionally blank 44 Lab 4: Cell Structure
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completing the virtual lab activity and your lab report. Refer to the lab rubric before you submit your report to your instructor. Cell Cycle Lab Safety Notes: Always handle microscopes and glass slides carefully. Wash your hands after handling the prepared specimens. Materials: Compound Light Microscope Glass microscope slide with prepared onion root tip specimen Purpose: understand and identify the stages of the cell cycle and mitosis. apply an analytical technique to estimate the relative
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involvement with textile merchants he worked with primitive microscopes. These simple scopes only had a magnifying power of three times and sparked Anthony to create a much more powerful lens’ which would make objects 200 times larger. Leeuwenhoek was also inspired to study microscopy by Robert Hooke’s illustrated book‚ Micrographia. During his lifetime‚ he made over 500 optical lenses. He also created more than 250 types of different microscopes. One of his most well-known glasses‚ called magnifying
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Title: preparation of films for staining Objective: to produce a thin smear of bacteria adhering to a clean microscope slide as preparatory to staining. Procedure: A. From broth cultures 1. Inoculating loop was sterilized using Bunsen burner and let to be cool before use it to obtain bacterial suspension from the tube. 2. The bacterial then placed in the center of the clean microscope slide. It also speared to produce thin films. 3. The films are set to air dry before wafting the slide gently
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cells. Tissue from an onion is a good first exercise in using the microscope and viewing plant cells. The cells are easily visible under a microscope and the preparation of a thin section is straight forward. An onion is made of layers‚ each separated by a thin skin or membrane. In this exercise you will make a wet mount on a microscope slide and look at the cells of the onion membrane magnified by the high power‚ compound microscope. We also can observe the actual structure of plant cells which consists
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OBSERVATION OF BACTERIAL CELLS VIA MICROSCOPE In this experiment‚ we have learned several basic molecular biology techniques. Here they are; Simple staining: Only one type of dye used for simple staining and it stains all cells the same color. Creating contrast between cell and background to observe cell morphology is aimed. Differential staining: A differential stain uses more than one dye and stains different kinds of organism with different colors. Positive staining: If cell itself is stained
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