Recombinant DNA Report Our final annotated gel image sums up the successful experiments we performed over the course of 8 weeks. The image will be referred to throughout the report: Lane 1: 10 µL of ladder. Lane 2: 20 µL of a pAMP- EcoRI/HindIII double digestion. Within the double digestion‚ one can find 8 µL of pAMP‚ 1 µL of the EcoRI enzyme‚ 1 µL of the HindIII enzyme‚ 5 µL of 10x Buffer 2.1‚ and 35 µL of water. A total volume of 50 µL was present
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DNA STRUCTURE AND REPLICATION One may wonder how a single cell becomes two cells‚ and why this is ultimately important to life. For this essay‚ it would be much too difficult to discuss the process of cell division‚ which is the biological basis of life. That being said‚ this essay will examine more closely the precursor to cell division‚ also known as DNA replication. DNA replication is the process of copying a double-stranded DNA strand on a chromosome within a cell. The process‚ in its totality
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An Introduction to DNA microarrays Rebecca Fry‚ Ph.D. http://www.buffalo.edu/UBT/UBT What is a DNA Microarray? genes or gene fragments attached to a substrate (glass) Tens of thousands of spots Hybridized slide Two dyes Image analyzed 1 The Beginnings of Microarray Technology Lockhart et al.‚ 1996 Nature Biotechnology “Expression monitoring by hybridisation to high-density oligonucleotide arrays” Schena et al.‚ 1995 Science “Quantitative monitoring of gene expression
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Atoms and Molecules Basic units of EVERYTHING! Which of these is true? 1. “Atom” and “molecule” mean the same thing. 2. Atoms are made of molecules. 3. Molecules are made of atoms. What is the smallest structure in this list that can be seen with an ordinary desk microscope? 1. 2. 3. 4. Cells. Cell nucleus. Atoms. Chain molecules‚ such as proteins. Atoms In our model of scale‚ remember that the BB represented an atom. Electrons Hydrogen Helium Neutrons Protons As a class‚ identify
Free Atom Chemical bond Electron
DNA FINGERPRINTING DNA fingerprinting is a method of identification that compares fragments of deoxyribonucleic acid. It is a technique used to distinguish between individuals of the same species by using only samples of their DNA. It is also called DNA typing. DNA is the genetic material found within the cell nuclei of all living things. In mammals‚ the strands of DNA are grouped into structures called chromosomes. Unless dealing with identical twins‚ the complete DNA of each individual is unique
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Legal Studies Essay DNA Profiling Breakthroughs in DNA testing have brought success to what would have otherwise been unsolved cases. DNA profiling is a technique used by many scientists and police to match DNA samples found at the scene of a crime with their respective counterparts generally found on their database. DNA profiling has helped match blood and semen samples found at the scene of a crime to the perpetrator‚ managing to sometimes solve cold cases that have been closed for decades
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particular colour combination that can be used is complementary colours. Complementary colours are traditionally‚ the colours that appear directly opposite each other on a typical colour wheel. It has also been recently re-defined to be‚ the wavelengths of light that come together at full intensity to produce white light (Pridmore‚ 2009). Examples of complementary colours are common pairs such as yellow-purple‚ red-green and orange-blue. When complementary colours are used together‚ they help contrast
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one thing that unites us all‚ DNA. DNA‚ standing for Deoxyribonucleic Acid‚ is commonly compared to a blueprint. This blueprint orders a highly specific set of corresponding bases‚ which in turn codes for an amino acid. However‚ this specificity comes at a very high risk; a single error in a base could code for a completely different amino acid‚ causing a mutation that could devastate the organism. Recently‚ scientists have discovered a new method to edit and repair DNA that could hopefully complement
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DNA profiling is a method of identifying an individual by unique characteristics of their DNA. A specific DNA pattern‚ called a profile‚ is obtained from an individual or a sample of tissue. This allows the comparison of the base sequence of two or more DNA samples to determine whether they are related. DNA profiling has many uses‚ in prevention of economic fraud‚ dietetic work‚ and classifying species‚ identifying bodies‚ forensic science‚ screening for disease‚ and investigating paternity.
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Deoxyribonucleic Acid (DNA) DNA stands for deoxyribonucleic acid‚ it is located in the nuclei of cells which make up the body. DNA is quite often referred to as one of the building blocks of the body.. It is made up of four bases known as: • Adenine • Guanine • Cytosine • Thymine James Watson‚ Francis Crick‚ Maurice Wilkins‚ Rosalind Franklin Crick and Watson‚ together with Maurice Wilkins‚ won the 1962 Nobel Prize in physiology or medicine for their 1953 determination of the structure of
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