DNA Profiling for Crime Investigation Rabia Awan Preston University Islamabad‚ Pakistan rabiawan079@gmail.com Abstract— DNA profiling also called as DNA typing or Gene fingerprinting has been used as a powerful process for identification of humans. Many countries around the world where forensic databases have created to store DNA patterns(DNA profiles) of crime scenes in order to put the actual criminals behind the bars and free the innocent who have been kept in jails for years without any
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compounds are soluble in water. This is due to the fact that water molecules are polar. Meaning that they have dipole bonds‚ which have a slightly positive and slightly negative end due to electronegativity differences between atoms. When ionic bonds are placed in water‚ they dissociate into their ions (positive and negative) due to the process of dissociation. These ions are then attracted to different dipoles present in the water molecule‚ in other words the negative ions are attracted to the positive
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Associate Program Material DNA Worksheet Answer the following in at least 100 words: 1. Describe the structure of DNA. DNA really stands for Deoxyribonucleic acid. DNA is a right handed double helix. Nucleotides that mount together by phosphodiester bonds make up DNA. It also contains a spiral strand that is made of phosphate backbone and secure bases (Adenine‚ Guanine‚ Cytosine‚ Thymine). Cytosine and Thymine are Pyrimidines‚ while Adenine and Guanine are Purines.
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Associate Program Material DNA Worksheet Answer the following in at least 100 words: 1. Describe the structure of DNA. * * There has been a major effort in the history of science to figure out the structure of DNA. Having a double standard helix DNA has a uniform a diameter in its entire length. The helixes fit within a defined three dimensional space because they are both right handed. Polynucleotide chains are held together by the bases in the (center) hydrogen bonding
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the structure of DNA they wanted to use the information to help them identify how DNA is replicated. Three different theories of replication were proposed by Watson and Crick. The semi-conservative model‚ where the DNA strand splits into two halves‚ which will then create a new DNA strand consisting of the old original half and a new half. The conservative model where the whole of the original DNA strand acts as a template and is replicated to make a completely new strand of DNA. The dispersive model
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Experiment 2 Titles Extraction of Bacterial Plasmid DNA and Analysis of Extracted DNA Samples Objectives * To learn the procedures needed in extracting the bacterial plasmid DNA * To determine the concentration of original DNA sample and purity of prepared DNA sample by using spectrophotometer * To analyze the extracted DNA sample by gel electrophoresis Materials and methods (Refer to UDBB2144 Laboratory 2A Manual Principles of biotechnology page 6-10) Results
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neutrophils‚ and the CBC that was performed indicated that the number of neutrophils in his circulation was increased. What role do neutrophils play in the resolution of a bacterial infection? In the course of your answer explain terms such as adhesion molecules‚ diapedesis‚ chemotaxsis‚ opsonization‚ and phagocytosis. 3. Jason’s physician noted that Jason’s cervical lymph nodes were enlarged‚ a condition referred to as lymphdenopathy. Describe the structure and function(s) of lymph nodes‚ and list the
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History of DNA Technology During the early1960s‚ there was great progress being made in beginning to understand the structure of genes and the mechanisms of their replication‚ expression‚ and regulation in prokaryotes and the viruses that began to infect them (Berg 2010). However at the time it was still unknown as to whether or not these findings applied to eukaryotes. This is because the tools used at the time for exploring genetic properties were not fit for the task. By the spring of 1972‚ the
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In this coursework I will be exploring two issues‚ my major issue being DNA Fingerprinting and my minor issue is PCR (Polymerase Chain Reaction). DNA Fingerprinting (Obtained from www.anselm.edu/.../genbio/geneticsnot.html) (The diagram above shows that the defendant had the victim’s blood on his clothes) Web Description: A method of comparing the genetic similarities or differences between individuals. This technology is often used as a forensic tool to identify the source of blood
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Background on Genomic DNA Isolation and Purification Generally‚ all methods involve the disruption and lysis of cells. This is followed sometimes by the removal of RNA (by RNAses‚ salt or other methods). Choosing which method to use will depend on many selection factors including: DNA is isolated from proteins by several methods including digestion of proteins by the enzyme proteinase K. Proteins are removed subsequently by salting-out‚ organic extraction‚ or binding of the DNA to a solid-phase support
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