employed by bacteria as restriction sites are not only found in all types of DNA but they all share a similar design wherever they are found. Most restriction sites are palindromic. They exhibit twofold rotational symmetry. This means that the two strands within the restriction site will have the same sequence if read in a 5’-3’ direction (Diagram 1). Different restriction enzymes will act upon different palindromic sequences but the nature by which they cut the DNA is always the same. Restriction
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needs to expand the underground Toronto Transit Commission (TTC) subway in order to ensure pubic transit is a viable option for individuals in the future by making it will make the daily commute easier and faster‚ reduce pollution and provide a more cost effective method of transportation. The current Toronto subway system needs to make a commitment to expand its lines north‚ east and west so that the citizens can be encouraged to choose the TTC giving them an easier‚ faster and more reliable method for
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QUESTIONS AND ANSWERS SECTION A: SIMILARITIES‚ DIFFERENCES‚ AND DEFINITIONS Gametes are mature haploid reproductive cells that unite to form a zygote that develops into a new individual. Gametes are also called sex cells. An egg or ovum is a haploid female reproductive cell or gamete whereas a sperm is a male haploid reproductive cell. A zygote is the initial cell that is formed as a result of the union of a male and a female gamete by means of sexual reproduction. The two haploid cells merge
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nucleus) 5. The diagram below shows the process of DNA a. Replication 6. The main enzyme involved in linking individual nucleotides into DNA molecules is d. DNA polymerase 7. The process by which the genetic code of DNA is copied into a strand of RNA is called b. transcription 8. In messenger RNA‚ each codon specifies a particular c. amino acid 9. Changes in the DNA sequence that affect genetic information are known as b. mutations 10. An expressed gene is one that b. is
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happen‚ ultimately increasing the rate of respiration. Triphenyl Tetrazolium Chloride (TTC) is a redox indicator often used in biochemical experiments to indicate cellular respiration. It is a white crystalline powder that can be dissolved in water‚ acetone and ethanol. TTC is colorless when oxidized and pink when reduced. TTC can be used to show the presence of active dehydrogenase enzymes by a change in color. TTC is heat and light unstable. During respiration‚ hydrogen ions or protons are released
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market value of any property received in relation to a CGT event‚ or which the taxpayer is entitled to receive. Under s110-25‚ cost base include several elements such as purchase price‚ incidental costs‚ the costs of ownership (for assets acquired after 20/08/1991)‚ capital expenditure. More specifically‚ under s110-25(3)‚ the incidental costs incurred to acquire the CGT asset or that relate to CGT event (for example‚ the disposal of a CGT asset). In this case‚ Party Time entered into a contract
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Gross Capital Gain * CGT Discount = 0.87 * 50% = $ 0.435 million Computation of Capital Gain and Capital Gain Taxes for the year 2013-14 As the other property was sold on 9th April 2013‚ that is after 31st March 2013 so the financial year changes from 2012-13 to 2013-14. Unit – 2: Capital Proceeds or Sale Value $ 1.45 million (-) Cost Base or Cost of Acquisitions $ 0.48 million Gross Capital Gain $0.97 million Net Capital Gain = Gross Capital Gain * CGT Discount = 0.97 *
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the deferral of losses that arise from business activities that are of a non-commercial nature. 3 Issue of CGT exemptions and reliefs: Legislation/Rule: In regards to the exemptions and reliefs available for the Capital Gains Tax‚ the existence of a capital gains asset and the occurrence of a Capital Gains Tax event must be identified first. Section 108-5 of the ITAA1997 that a CGT asset is referred to as ‘any kind of property;’1. Section 104-10 of the ITAA1997‚ covers the
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Name: Anna Tran Date: August 7‚ 2015 Student Exploration: Building DNA Vocabulary: double helix‚ DNA‚ enzyme‚ mutation‚ nitrogenous base‚ nucleoside‚ nucleotide‚ replication Gizmo Warm-up The Building DNA Gizmo™ allows you to construct a DNA molecule and go through the process of DNA replication. Examine the components that make up a DNA molecule. 1. What are the two DNA components shown in the Gizmo? Nucleosides and phosphates 2. A nucleoside has two parts: a pentagonal sugar (deoxyribose)
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sequences to Thread or Dynamin-related protein 1 (drp-1)‚ along with T7 promoter site sequences. The amplified DNA was purified and both strands of the DNA were used as templates for in vitro transcription reactions‚ producing complementary RNA strands for each of the two genes. The RNA was heated‚ to separate the RNA hybrids and then the complementary strands were allowed to anneal‚ forming double-stranded RNA molecules. The purified double-stranded RNA produced in lab was then used with THREAD
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