Abstract: During the lab a better agent for absorbing Procion Red Dye was trying to be found. Using the different dilutions of a stock solution and a spectrophotometer an Absorbance vs. Concentration graph was created and using the trendline from that graph it was determined which agent‚ zeolite‚ magnetic zeolite‚ or charcoal were better at absorbing the dye. From the results collected it was found that charcoal was the best at absorbing the dye‚ when moles of dye per gram of agent used were calculated
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involves measuring the absorbance of several concentrations of the pure substance or the "standard" substance determine relationship between concentration and absorbance compared results from unknowns How to use Linear Regression for Generating a Standard Curve? © 2010 by M. Olaveson UTSC 2 BIO A01F-Fall 2010 - ASSIGNMENT # 2 - Preparing a Standard Curve using Excel 2007 Assignment # 2 Analysis of Data from Lab 2-Exercise 2 Table 2.6. Protein in Test Tubes prepared for
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The Effect of Concentration on Absorbance Background Information The purpose of the “Determining Solution ‘Concentration’ Using A Spectrophotometer” lab was to use a spectrophotometer to find the relationship of concentration and absorbance obeying the Beer-Lambert law‚ which states concentration and absorbance are directly related‚ to then further determine the concentration of three unknown solutions. With the assumption that the solutions obey the Beer-Lambert law it is predicted that as concentration
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protein concentration of an unknown sample of BSA‚ and by using the standard curve. Abstract A spectrophotometer’s purpose is to use colors of the light spectrum to determine the concentration of light absorbing molecules in a solution. (p.59) In this particular lab‚ our mission was to determine the protein concentration and the standard curve of the unknown sample of BSA. This‚ by preparing five dilutions of the unknown solution of BSA together with other known concentrations‚ and then
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Abstract: The purpose of this experiment was to determine the concentration of an unknown amount of copper sulfate using the spectronic 20. Four solutions of different concentrations of H₂O and CuSO₄ were put into the spectronic 20 to observe their absorption of light. Then‚ a graph was created to plot down the measurements. Using the calibration curve of the graph‚ the concentration of the unknown substance was found out to be .12M. Background/Introduction: Beer’s law (which states that
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flask using a volumetric pipette. The volumetric flask was then filled to the mark with distilled water. The flask was covered with Parafilm and inverted to mix. The next part of the experiment was to determine the Vitamin C in a solution of known concentration. Observations of the Vitamin C sample and the amount found on the label was recorded. A tared piece of weighing paper was used to weigh 0.27 g of Vitamin C. The Vitamin C was then transferred to a clean 50 mL volumetric flask. The flask was filled
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Acid –Base Titration: A volume of 128 mL of 0.650 M Ba(OH)2 was required to completely neutralize 50.0 mL of nitric acid solution (HNO3). What is the concentration of the acid solution? Gravimetric Titration: Calculate the molar concentration of Ba2+ ions in a 500.0 mL sample of an unknown aqueous solution if 2.47 g BaSO4 is formed upon the addition of excess Na2SO4? Stoichiometry of Acid –Base Reactions: What volume of 0.800 M HCl is required to completely neutralize 35.0 g of Ca(OH)2
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the pH of bromothymol blue affects the absorbance value of the solution which determines the equilibrium constant (pKa) of the indicator. Variables: Variables Variables Measured Method of measurement Independent pH of the six buffer solution A pH probe attached to a data-logger will be used to measure pH Dependent Absorption of the buffer solutions at wavelength 435.0nm and 617.0 nm A spectrophotometer (±0.001) will be used to measure absorbance at wavelength 436.0nm and 617.0 nm respectively
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1. The label CORROSIVE on a chemical container indicates Ans : ( b ) that contact destroys living tissue as well as equipment 2. FLAMMABLE means Ans : ( a ) easily ignited and capable of burning rapidly 3. " Fatal if swallowed " indicates Ans : ( c ) that the substance will cause death if ingested 4. What is the correct way to handle the following situation? Briefly explain. a. Chemical : Acetic asid i. Inhalation exposure Ans : Remove the person from the exposure. Begin rescue
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really constant. Fe3+ (aq) + SCN– (aq) ←→ FeSCN2+ (aq) A big part of this lab includes understanding (and in the end proving) that absorbance is directly proportional to the concentration of a solution (Beer’s Law)‚ and if Absorbance v. Concentration is graphed‚ a straight line will result. The regression line’s equation is used to determine the concentration of an unknown solution once the percent of T has been measured. The equation of Beer’s Law (A=abc) is an efficient way of
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