cuvette and measured the absorbance to 595nm. Using the BSA stock solution (2mg/mL=2000 ug/mL)‚ we prepared the concentration of the protein standard by carrying out serial dilution at 2x‚ 5x‚ 10x‚ 20x and 40x. The 2x dilution was made by mixing 250 uL of BSA and 250 of the homogenizing buffer. The solution was then vortexed for 10 seconds. A similar procedure with different concentration of BSA and stock solution was carried out to find the other diluted concentrations. After the dilutions were
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by Q laboratory to determine their absorbance and prepare a Beer’s law plot. Molarity of these samples was also calculated to determine concentration and percent error rate. Students also analyzed the concentration of blue dye #1 to determine the concentration of blue dye #1 in a commercial blue dye drink. Procedure Exercise #1 Step #1: Convert %T (Table 1) to absorbance and prepare a Beer’s law plot using the data. Step #2: Determine the concentration of M of the samples in table 2: A=ebc
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then distilled‚ and diluted into 4 containers to obtain concentrations of 100%‚ 75%‚ 50% and 25%. There were 6 set-ups‚ each with 10 cockroaches‚ one positive control set-up‚ in which the group used commercial pesticide‚ one negative control set-up‚ containing water‚ and one each for the different concentrations of the extract. The experimental results provided evidence for a conclusion that the extract with the higher concentration killed more cockroaches. There is a significant difference
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using colorimetric analysis Materials On the sheet attached Procedure On the sheet attached Results Standard (mg/L) | Absorbance | 10.0 | 0.354 | 7.5 | 0.308 | 5.0 | 0.230 | 2.5 | 0.193 | 0.0 | 0 | unknown | 0.161 | Calculations 1. What is the concentration of phosphorus ions‚ in mg/L‚ in the initial 250 mL of fertiliser solution? y = 0.0399x Absorbance of unknown = 0.161 0.161 = 0.0399x x = 4.035 4.035 x 10 = 40.35 = 40.4 mg/L 2. Use
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Daphnia heart rate Independent: caffeine concentration Dependent: heart rate of Daphnia Measuring the content of Vitamin C in fruit juice Independent: fruit juice Dependent: volume of juice required to decolourise 1cm3 of DCPIP The effect of temperature on cell membranes Independent: temperature of water Dependent: % transmission of light through resulting solution The effect of changing enzyme concentration on rate of reaction. Independent: concentration of enzyme dependent: time taken for enzyme
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would support the initial rationale hypothesis that if salt concentration is not equal to the optimum concentration of 0.15M‚ then its ability to function will decrease. 0.15M with the trypsin added had the lowest absorbency compared to the three other concentrated groups that were tested (0.0M‚ 0.075M‚ 0.30M). Closer comparison that if you take the results obtained from trial one with trypsin at a salt concentration of 0.075M‚ the absorbance was 0.165 (440 nm wavelength). In comparison to 0.15M‚ which
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Results The results presented by the graph showed that there was a positive relationship/trend between temperature and light absorbance. The higher the temperature of the water surrounding the beetroot‚ the more coloured the water around the beetroot was. This is shown in the graph by: at 0°C‚ the water surrounding the beetroot was fairly pink and had an averaged amount of 0.074 absorbency. At 80°C‚ the water surrounding the beetroot was very pink almost red‚ and had an average of 0.982 absorbency
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laboratory to determine their absorbance and prepare a Beer’s law plot. Molarity of these samples was also calculated to determine concentration and percent error rate. Students also analyzed the concentration of blue dye #1 to determine the concentration of blue dye #1 in a commercial blue dye drink. Procedure: To drop solutions into the test tubes and then determine the Beer’s Law relationship of each and the calculations of each of part of the experiment. Convert %T to absorbance and prepare a Beer’s
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out filter and weight the sample and filter. Heat and weigh repeating until it is constant. Concentration Absorbance 0.000764 0.030118 5.73E-04 0.027196 3.82E-04 0.009333 1.91E-04 1 7.64E-05 1 Discussion and Errors: The main error that occurred was that my partner and I entered the wrong numbers in the Concentration section resulting in incorrect data. We put the incorrect concentration levels and we had no time to correct it. Everything in the calculation of the empirical formula
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Concentration of a Solution: Beer’s Law The objective of this experiment is to determine the concentration of an unknown copper sulfate solution. You will be using the Colorimeter. In this device‚ red light from the LED light source will pass through the solution and strike a photocell. A higher concentration of the coloured solution absorbs more light (and transmits less) than a solution of lower concentration. The Colorimeter monitors the light received by the photocell as either an absorbance
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