The enzyme tyrosinase was successively extracted by combining a homogenate of a potato and sodium sulfate with ammonium sulfate. Tyrosinase was successfully extracted by taking advantage of solubility properties of certain proteins. A standard curve was generated indicating dopachrome absorbance values through the use of a spectrophotometer and a computer graphing program. A spectrophotometer was used to measure either the amount of light that passed through a solution (transmittance) of the amount
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Investigating the Relationship between Concentration of Sodium Chloride and the Rate of Reaction of Enzyme Amylase Research Question: How will changing the percentage of sodium chloride concentration affect the rate of reaction of enzyme amylase‚ measured using the absorbance of starch and iodine with a spectrophotometer. Introduction: Amylase is an enzyme that is involved in the human digestive process. Found in both the human pancreas and the human saliva‚ amylase breaks down starch into sugar so
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Discussion The primary purpose of this experiment was to determine the optimum temperature range for the activity of the enzyme lactase. Extreme temperatures can have a detrimental effect on enzymes; very hot temperatures can cause the denaturation in the enzyme‚ which is the loss of protein structure. This causes a change in the shape of the enzyme leading to its inability to perform its function. As previously stated‚ the alternate hypothesis read: the optimal temperature range for lactase activity
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Enzymes are biological molecules (proteins) that act as a catalyst and help complex reactions occur everywhere in life‚ for example a piece of steak that is being digested into energy. Molecules found at the beginning of the process are called substrates‚ and these enzymes exchange them into differing molecules known as products. Nearly all-metabolic processes in a cell need enzymes in order to function at rates that are fast enough to sustain existence. Those who are lactose intolerant are simply
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Effect of the Changes in the Environment to the Functionality of the Enzymes Introduction a. Background In our everyday lives‚ enzymes are used in our bodies‚ and in nature around us‚ to speed up the chemical reactions happening constantly‚ which happens by lowering the amount of activation energy needed to start various reactions. The way this works is by attaching the particular substrate to the active site of the enzyme‚ where it will start to aid the chemical reaction. Then‚ the allosteric
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Sample Lab Report: Factors which Affect the Activity of the Enzyme Catalase Purpose: Must include: background information about concepts involved in the lab‚ statement of purpose of the lab identification of independent and dependent variables. A hypothesis is often not necessary or appropriate. Enzymes are proteins that speed up chemical reactions in cells. They break down molecules called substrates. Each enzyme has only one substrate that it breaks down. Enzymes are produced in
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Enzymes play a vital role in helping our body function. They act as biological catalysts and help speed up reactions that would otherwise take long periods of time to naturally occur. Enzymes help lower the activation energy required for the reactants to reach the transitional state from which then they can form products. However‚ enzymes do not change the free energy of the reaction. Enzyme’s ability to catalyze reactions comes from the shape of the active site on the enzyme. Enzymes are hyper-specific
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Enzymes are proteins that increase or decrease the rate of chemical reactions. They are generally globular proteins and are around 62 amino acids residues in size. What enzymes do is determined by their 2-dimensional shape. A lot of enzymes are bigger than the substrate they act on‚ but only a little part of the enzyme involved directly with the catalysis. Without enzymes the chemical reactions in the body‚ would be so slow‚ the body would shut down. And cell reactions would take too much energy
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Purpose: Restriction enzymes cut DNA at a certain palindromic sequence. Three samples of lamda DNA set up to be cut with restriction enzymes PstI‚ EcoRI‚ or HindDIII. There were also two more samples‚ one of these samples was not mixed with any restriction enzyme and the other was a marker‚ which used an enzyme which creates fragments with a known number of base pairs used to create a standard curve. All five samples were put through agarose gel electrophoresis in order to estimate the amount of
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The data from the experiment demonstrates that the catalase enzyme breaks down the hydrogen peroxide due to its harmful toxicity to the liver. In section A‚ the effect surface area has on the enzyme was tested. The results have proven that as the surface area increases‚ the reaction rate of the enzyme also increases. To illustrate‚ when the liver was ground‚ the bubbles from the reaction reached a maximum height of 150mm in five seconds less than the unground liver which merely reached a maximum
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