NAME: Aleema Chelsea Chinchamee LAB PARTNERS: Karishma Ramrattan‚ Vishma Ramsumair and Sharona Badree ID #: 814003081 DATE (of lab session): Tuesday 24th March‚ 2015 DEMONSTRATOR: Maurissa Course Code & Title: BIOL 1362- Biochemistry I Title of Lab: Investigating Enzymatic Activity in Sweet Potato‚ Irish Potato Extract and Milk. Aims: 1. To determine the effect of ascorbic acid on Polyphenol Oxidase (Phenolase). 2. To determine the level of specificity of Phenolase using the
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Minimum Inhibitory Concentration (MIC) is a way to measure the sensitivity of microbes to antibiotics or antifungals (antimicrobials). Organisms are tested for growth in various concentrations of antibiotic. This allows microbiologists to see which concentration (MIC) of antimicrobial will be effective against the pathogen. It also used for determining treatment for patients suffering from infections such as pneumonia‚ meningitis‚ endocarditis or osteomyelitis or managing the treatment of high-risk
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Enzyme Catalysis Lab Problem: Before the lab‚ one should understand: • The general functions and activities of enzymes; • The relationship between the structure and function of enzymes • The concept of initial reaction rates of enzymes; • How the concept of free energy relates to enzyme activity; • That change in temperature‚ pH‚ enzyme concentration‚ and substrate concentration can affect the initial reaction rates of enzyme-catalyzed reactions; and • Catalyst‚ catalysis‚ and catalase
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in Table 4. Bovine serum albumin (BSA) was diluted with distilled water. The absorbance of each sample were measured using spectrophotometer at 595nm. Standard protein curve was plotted according to the concentration of protein where the x-axis and y-axis represent protein standard concentration and absorbance (595 nm) respectively. 3.10.2 Protein Determination The treated and untreated samples were measured using Bradford assay. 10µl of the samples were made up to 100µl with distilled water
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Lab 6: Enzymes . header Purpose: The purpose of this lab is to test for enzyme activity‚ look at enzyme specificity‚ and how temperature affects enzyme activity. Time need to perform this lab: approximately 3 hours Preparation FIRST: Read the lab in its entirety TWICE before you begin. You will perform the experiment‚ write your lab report and include the answers to the additional 4 questions within the text for full credit on this experiment. Materials: •3% hydrogen peroxide •a
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Enzyme Lab 6 03/13/2013 Report by Mary Jo Anthony I. Introduction II. Materials and Methods III. Results IV. Conclusion and Discussion Introduction Background Information: This lab allowed us to study chemical reactions and how catalysts will affect the rate of these reactions. The reaction we studied is the breakdown of hydrogen peroxide to water and oxygen and it is vital to life. The molecule hydrogen peroxide is a molecule that is toxic
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Investigation on the effects of Temperature‚ pH levels‚ and Enzyme Concentration on the reaction rate of the Decomposition of Hydrogen Peroxide by Catalase The purpose of this investigation is to figure out how temperature‚ pH‚ and enzyme concentration affect the reaction rate of an enzyme. It’s important to understand how certain factors affect enzymes because of their crucial role in the metabolic processes of life. Enzymes lower the activation energy necessary for a chemical reaction to
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Cavanaugh Lab: Tuesdays at 1pm Enzyme Kinetics Lab Introduction: Enzymes are proteins that will catalyze reactions to make the rate of the reaction occur faster than it would without. It can also make the reaction occur in the first place. Tyrosinase is an enzyme that has a variety of functions and activities. It produces pigments like melanin and others that would be apparent when a fruit is cut in half and it browns. (Bien-etre 3).There is that one function that stands out and the enzyme is continuously
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Sang Kim Enzyme Catalyst Purpose/Problem: There are four parts to the Enzyme Catalyst lab - Activity A‚ B‚ C‚ and D. In activity A‚ the characteristics of enzyme actions will be observed. The main purposes are to determine the rate of an enzyme catalyzed reaction‚ to study the characteristics of an enzyme mediated reaction‚ and to observe the effect of heat on enzyme activity. The purpose of activity B is to use the Titration Protocol to determine the initial amount of H 2O2 present
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The effect of time on enzyme reaction. Abstract: In this lab investigation we will observe how the amount of hydrogen peroxide is affected by catalase over time. The enzyme was added to 10 mL’s of hydrogen peroxide and observed over time to determine the relation between time and enzyme activity. The hypothesis stated that as time increased substrate would decrease. Therefore I predicted that at 60 seconds‚ there would be the least amount of H2O2. The enzyme activity mirrored my predictions
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