saliva is to quantitatively measure the ratio of concentrations of caffeine and metabolites. There are three different metabolites of caffeine and the majority of caffeine metabolite is paraxanthine. The ratio of concentration represents the activity of the first metabolism enzyme of caffeine‚ CYP1A2 because it is responsible for the pathway of metabolism of caffeine. And the half-life of caffeine is about 4 to 5 hours (Thorn‚ 2011) Therefore‚ in this lab‚ we measure the saliva collected at different
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Enzymes All enzymes are globular proteins and round in shape They have the suffix "-ase" Intracellular enzymes are found inside the cell Extracellular enzymes act outside the cell (e.g. digestive enzymes) Enzymes are catalysts → speed up chemical reactions Reduce activation energy required to start a reaction between molecules Substrates (reactants) are converted into products Reaction may not take place in absence of enzymes (each enzyme has a specific catalytic action) Enzymes catalyse
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McLean 1 Creating Acetylene Gas Introduction The purpose of the lab was to determine the ratio of air to acetylene results in complete combustion of acetylene gas. The balanced chemical equation for this experiment was C2H2(g) + O2(g) --> CO2(g)+ H2O(l). Complete combustion is the reaction of an element or compound with oxygen to produce the most common oxides and energy. Complete combustion occurs when the fuel and oxygen combine in exact proportions to completely burn the fuel
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production and reaction velocity increased with increasing catalase concentration‚ however‚ the 33% percent catalase concentration showed a drop of 0.175 mL O2/s compared to the 25% catalase concentration (figure 1.2). The velocity of 25% catalase was 0.275 mL/s‚ 33% was 0.1 mL/s‚ 50% was 0.435 mL/s‚ and 75% catalase was 0.575 mL/s (figure 1.1). The 50% catalase concentration produced the most O2 overall however the 75% catalase concentration had the fastest initial reaction velocity. Experiment III: O2
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two Title: Enzyme Function Purpose: To observe the role of enzymes in chemical reactions and to determine the kinds of cells that contain more of the enzyme catalase. Prior Knowledge: Enzymes are proteins that assist the chemical reactions of a cell by lowering the amount of activation energy needed to start the reactions‚ thereby enabling the cell to carry out the reactions at a faster rate; enzymes that lower the activation energy are therefore called catalysts. Moreover‚ the enzyme itself is
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One of the main learning experiences of this lab is to learn the importance of calibrating a sensor. In most cases‚ a sensor will not have an output that is exactly what you need. An example of this is a strain gage. A stain gage gives back a voltage‚ but with that given voltage a distance can be found. Introduction: In this experiment‚ the QNET-MECHKIT was used. This board has multiple compact sensors that can be used for experiments. For this lab we will be using the strain gage to find the
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I.Purpose The purpose of this lab is to show how potential energy and kinetic energy is shown and transferred using a model rollercoaster. This lab also demonstrates the Law of Conservation of Energy. II.Introduction Potential and Kinetic energy have a very big relationship. The Law of Conservation of Energy states that “Energy cannot be destroyed or created‚ but can be transformed or transferred.” This lab will help demonstrate this law and show the conversion between Kinetic and Potential
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Nervous System lab was designed to measure reaction times by using a variety of tests that affect the heart rate. These tests were the startle‚ stroop‚ Valsalva maneuver‚ diving reflex‚ and a test of our choice. The test our group chose to measure the heart rate in response to doing a trust fall to anticipate the stress right before the fall. To begin this lab we chose a participant‚ and then hooked their finger up to the pulse transducer sensor. Using the chart 5 program on the lab computer the participant’s
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Lab 17 Amino Acids and Proteins Lab date 10/22/2013 12-1350 I Purpose The purpose of this experiment was to separate mixtures of II Method For a complete list of experimental procedures see prelab outline attachment #1. “For a complete list of experimental procedure see Seager‚ Spencer L. and Slabaugh‚ Michael R. Safety-Scale Laboratory Experiments for Chemistry for Today General‚ Organic and Biochemistry; Thomson Brooks/Cole‚ Belmont‚ CA‚ USA‚ 2008; pp.221-225”. III Data Part A Mass
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Objective: 1. To determine the wavelength of maximum absorption‚ Amax of bromophenol blue. 2. To construct a standard concentration curve for bromophenol blue. 3. To determine the concentration of the unknown bromophenol blue solutions. 4. To determine the concentration of two different solutes‚ bromophenol blue and methyl orange‚ in a mixture. Material and method: Refer to practical manual page 7 Results: Part 1: Determination
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