VARYING EFFECTS OF ENZYME CONCENTRATION ON REACTION RATES OF MALATE DEHYDROGENASE CELL BIOLOGY 13 NOVEMBER 2007 Enzymes are biological catalysts. They are proteins that speed up reactions with low concentrations. These enzyme proteins are made up of linkages of amino acids. The links coil‚ and coil again forming a tertiary structure. This structure has a groove in it called an active site. The active site is
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Data collection Quantitative Data Raw Data Table 1: Table showing the mass of the amount of unknown acid X measured in grams (±0.001g) Table 2: Table of reading of the burette initially filled with 25mL of 0.201moldm-3 sodium hydroxide (NaOH) to titrate 25mL (±0.03mL) of unknown acid X in mL (±0.05mL) after each titre. Reading on the burette initially filled with 25mL of 0.201moldm-3 NaOH (±0.05mL) First titre 21.3 Second titre 18.2 Third titre 15.2 Fourth titre 12.0 Qualitative
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firstly amplified by PCR‚ then cloned in the pBluescript plasmid vector. EZH2 is an enzyme which adds methyl groups to the twenty seventh amino acid (Lysine) and is encoded by EZH2‚ the EZH2 gene encodes part of the Polycomb group which make protein complexes that help to maintain genes transcriptional repressive state over successive cell generations. http://www.ncbi.nlm.nih.gov/gene/2146 Throughout this lab report template DNA that contains the gene EZH2 was provided‚ this will be amplified by
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a variety of foods. These foods are required for energy and human growth. Proteins are a diverse group of macromolecules with many different functions (Mbuthia‚ 2012). Many are structural components of bone‚ muscle‚ hair‚ tissue‚ etc. Others are enzymes that speed up cellular reactions. All proteins are made up of amino acids (Johnson‚ 2010). The purpose of this experiment is to determine whether peptide bonds are present in various food substances. The color violet will indicate the presence of
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simple mechanisms used to convert rotary motion into oscillating linear motion and vice-versa. The first of these is the slider-crank - a mechanism widely used in engines to convert the linear thrust of the pistons into useful rotary motion. In this lab we will measure the acceleration of the piston of a lawn mower engine at various speeds. The results exemplify a simple relation between speed and acceleration for kinematically restricted motions‚ which will discover. An adjustable slider-crank apparatus
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Abstract This lab’s purpose was to test the patterns of percolation in various places. This lab tested how soil would absorb water based on different places. Distance from a group of trees‚ distance from a swamp‚ and different types of soil were tested. From the results collected‚ you can not prove‚ but you can infer that the ability to absorb increases as you go farther away from a swamp‚ and a group of trees. The ability to absorb also increases based on how much silt is in soil. Introduction
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Enzymes Enzymes are… * Biological catalysts Lower the energy level needed for a biochemical reaction to occur. This energy level is called activation energy. * Proteins Polypeptide chains made up of 100’s-1000’s of amino acids in a specific sequence. * Do not get “used up” in a reaction The number of “uses” of an enzyme depends on the enzyme. * Work more efficiently at certain optimum temperatures. * They are “reaction-specific”. Each enzyme is included in one reaction.
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Coulomb’s Law Purpose: The purpose of this lab was to demonstrate that the force between two stationary charges is directly proportional to the product of the charges and inversely to the square of the distance between them. Coulomb’s law tells us that the force between two charges depends (1) linearly on the strength of each charge‚ and (2) inversely on the square of the distance between them. Mathematically we would write this as . Procedures Part1 Begin by removing the right side
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influencing multiple (and possibly unrelated) phenotypic traits. Mutation in this type of gene will simultaneously affect more than one trait. An example is phenylketonuria‚ a human disease caused by mutation(s) in a single gene that codes for the enzyme‚ phenylalanine hydroxylase. The disease is characterized by mental retardation and reduced hair and skin pigmentation. List and explain other factors that
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This lab has introduced the use of daphnia‚ pill bugs‚ amoeba and Uglena. We the AP Biology students were first introduced to the test subjects to test his or her capability of handling living organisms for upcoming science experiments. The AP Biology students were asked to create original experiments for all test subjects testing reactions to gravity‚ light intensity and reaction to food. Daphnia‚ or Daphnia magna‚ are microscopic organisms in which the students created an original lab to test
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