Abstract Enzymes are proteins that lower the activation energy needed for chemical reactions. The two main environmental factors that can affect the enzyme’s activity are temperature and pH‚ and each enzyme works best at a particular temperature and pH. The purpose of this enzyme kinetic experiment was to observe the effect of temperature and pH on the reaction of barley alpha-amylase enzyme with starch substrate and establish the optimum temperature and pH for this reaction. The optimum temperature
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conducted on 13th August 2008 in Machines Dynamics Laboratory. The experiment was conducted in groups of four‚ and was supervised by lecturer Mr. Mohd Azahari Johan. Conducting this experiment is for fulfilling the requirements of Applied Mechanics Lab (MEC 424). A pendulum is defined as body so suspended from a fixed point as to swing freely to and from by the alternate action of gravity and momentum. It is used to regulate the movements of clockwork and other machinery. Therefore‚ a compound
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central role to establish the basic concept in coordination chemistry while reaction kinetics of coordination compound is known as the area of continuing intense research activity. The mechanism of the reactions of coordination compounds relevance to enzyme reactions where the active site is being involve in coordination to a metal ion. PROCEDURE: (A) Preparation of Trans-Dichlorobis(ethylenediamine)cobalt(III) chloride 1. About 16g of cobalt(II)chloride hexahydrate is dissolved in 50ml of distilled
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the period of oscillations to the length of the pendulum.Sources of error for this procedure included precision in both length and time measurement tools‚ reaction time of the stopwatch holder‚ and the accuracy of the stopwatch with respect to the lab atomic clock. The final result of g takes into account the correction for the error introduced using the approximation. There are opportunities to correct for the effects of mass distribution‚ air buoyancy and damping‚ and string stretching[1]. Our results
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FOOD NUTRITION BASICS LABORATORY REPORT LAB 3 PROTEINS DUE DATE Next FNB Practical NAME (CAPITALS) ______________________________GROUP LETTER___________ This work is the product of my own efforts and has not been copied from any other sources except where full acknowledgement has been given. Signed________________________________________ Student No._____________ Introduction The principle involved in this experiment is denaturation. It involves loss of ordered structure and can be caused by changes
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# Now You See It – Copper Cycle Lab The purpose of the lab is to discover what happens when someone executes a series of procedures‚ beginning with copper metal. What is done | What is observed | 1. Started with copper‚ Cu (s). | reddish‚ brownish‚ orange-ish‚ powder-like | 2. Added nitric acid‚ HNO3 (aq). | acid turns blue and smells like chlorine. | 3. Added water‚ H2O (l). | stayed the same | 4. Added sodium hydroxide‚ NaOH (aq). | changed consistency‚ gel-like | 5. Heated the
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Enzymes (pron.: /ˈɛnzaɪmz/) are large biological molecules responsible for the thousands of chemical interconversions that sustain life.[1][2] They are highly selective catalysts‚ greatly accelerating both the rate and specificity of metabolic reactions‚ from the digestion of food to the synthesis of DNA. Most enzymes are proteins‚ although some catalytic RNA molecules have been identified. Enzymes adopt a specific three-dimensional structure‚ and may employ organic (e.g. biotin) and inorganic (e
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Effects of pH on fungal amylase activity BI 211 November 25‚ 2011 Introduction In recent years‚ the uses of microorganisms have become a huge importance to industry and sparked a large interest into the exploration of enzyme activity in microorganisms. Amylase is one of the most widely used enzyme required for the preparation of fermented foods. Apart from food and starch industries‚ in which demand for them is increasing continuously‚ amylase is also used in various
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the acyl-enzyme is then hydrolysed by nucleophilic attack of serine on the peptide carbonyl group‚ collapse of the tetrahedral intermediate and the release of the amine component. Histidine 57 then acts as a general acid catalyst and draws a proton away from the water molecule. The OH- ion then attacks the carbonyl carbon atom of the acyl group which forms another tetrahedral intermediate which breaks down to form the carboxylic acid product. The release of this acid then frees up the enzyme to continue
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PEES 4200W- Physiology of Exercise Lab #2: Metabolism and Energy Expenditure 1. The Respiratory exchange ratio is the ratio of the volume of carbon dioxide produced and oxygen consumed. The ratio indicates the energy that the subject is expending for indirect calorimetry‚ how efficient the subject ’s body is at utilizing the oxygen inhaled‚ as well as the main substrate being used for energy during varying intensities of exercise. During rest the volume of carbon dioxide was 0.73L/min‚ the volume
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