Chirag Patel Biology 157 Determining the Effect of Varying Substrate Concentration Question: How does concentration of potatoes affect the amount of oxygen produced with hydrogen peroxide and potatoes (enzymes)? Introduction The enzyme used for this experiment is Catalase. Catalase is inside mostly any living organism which uses oxygen. Its job is to break down hydrogen peroxide‚ into oxygen and water. (Formula) 2H2O2 ---> 2H2O + O2 (lab manual). There are limiting factors which if altered
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Substrate concentration and yeast catalase Aim: To see how the substrate concentration in hydrogen peroxide affects the rate of an enzyme controlled reaction using yeast catalase. Introduction: An enzyme is a biological catalyst made of protein. Enzymes are protein molecules found in living organisms and in this case I will use a yeast catalase. Catalase is an enzyme that catalyzes the reduction of hydrogen peroxide. Hydrogen peroxide is a poisonous by-product of metabolism‚ so it is very
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Conclusion I predicted that if the decolorization of DPIP is caused by photosynthesis and not cell respiration and spinach extract containing chloroplasts and mitochondria is incubated with DPIP‚ then the rate of DPIP decolorization should be higher if in bright light verses dark light because DPIP is reduced by photosynthesis and not by the mitochondria or any other cellular function. If DPIP was only decolorized by chloroplasts‚ then the percent transmittance of chloroplast suspensions would be
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Abstract The process of photosynthesis can be summarized as the photoautotroph consumption of carbon compounds as food and release of oxygen compounds as waste. The methods to measure the rate of photosynthesis are various. One method utilizes CO2 sensors to measure photoautotrophs CO2 intake under various luminescent environments. An elementary method utilizes H2O and NaHCO3 (Sodium Bicarbonate) for submersion of deoxygenized photoautotroph specimens to measure the release rate of oxygen compounds
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Photosynthesis Lab Gizmo website: www.explorelearning.com Class code: EFVNHTVRGY Gizmo Warm-up During photosynthesis‚ plants use the energy of light to produce glucose (C6H12O6) from carbon dioxide (CO2)‚ and water (H2O). Glucose is a simple sugar that plants use for energy and as a building block for larger molecules. A by-product of photosynthesis is oxygen. Plants use some of the oxygen they produce‚ but most of it is released. In the Photosynthesis Lab Gizmo™‚ you
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BIOLOGY DCN PHOTOSYNTHESIS LAB # 6 Effect of varying coloured filters on the photosynthetic rate of spinach chloroplasts The data below is just representative of what trends and relationships you were supposed to see. Numbers can vary. Absorbance at 620 nm for each treatment DCPIP + chloroplasts t=0 min. 0.93 0.945 0.905 0.915 t=3 min. 0.95 0.731 0.83 0.816 change in A620 -0.02 0.214 0.075 0.099 t=0 min. t=3 min. change in A620 t=0 min. t=3 min. change
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First recognized as a chemical compound in 1818‚ hydrogen peroxide is a clear‚ colorless liquid most often used for cleaning or disinfecting(Britannica‚ 2013). Since pure hydrogen peroxide is unstable‚ it is found in stores as an aqueous solution. The chemical formula for hydrogen peroxide is H₂O₂ which means there are two hydrogen atoms and two oxygen atoms in the compound(Liebeskind‚ 2013). Seed germination is simply the process in which a seedling sprouts from a seed and begins it’s growth. The
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Name ____________________________ Date ____________ THE EFFECT OF CONCENTRATION ON THE RATE OF DIFFUSION PURPOSE: This experiment will explore the concentration gradient by measuring the rate of diffusion in a number of theoretical cells. The objectives for this lab are: (1) To define concentration gradient‚ (2) To explain experimental data concerning different concentration gradients‚ (3) To complete a data table and prepare a graph to illustrate the data‚ and (4) To apply information from
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Photosynthesis and Respiration Unit2 – IP 1. A) Photosynthesis space is a process in which the energy from light is used to produce carbohydrates. Aside from using light energy‚ photosynthesis includes carbon the oxide and water. A1) In order to generate energy aerobic respiration requires oxygen‚ although fats and proteins and carbohydrates can be consumed and processed as reactant‚ is the usual method of breaking down in glycolysis and usually requires the pyruvate to enter the mitochondrion
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anabolic reaction. There are five factors that effect enzyme activity. They are: ·Temperature ·pH ·Inhibitors ·Substrate Concentration ·Enzyme Concentration The factor I am changing is surface area of potato containing Catalase. By increasing the surface area of the potato‚ you make more Catalase molecules be exposed to hydrogen peroxide. This will result in the rate of reaction increasing because it increases the chance of more successful collisions between the enzyme and the substrate. Catalase
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