Amino acids are important building blocks; they play an essential role in the formation of proteins and as intermediates in metabolism. Amino acids can serve as cell signaling molecules and can regulate gene expression. An optimal amount of amino acids is essential for health. There are over 500 amino acids‚ however only 20 are commonly found. Amino acids all have an amine functional group and a carboxylic acid functional group. They differentiate from each other in their side chains. Only 10 of
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Result: Part A: Table 1: Fractionation of amino acid Fraction No | Response to Ninhydrin test(level of blue colour change) | Response to Sakaguchi test (colour change) | 1 | X | yellow | 2 | Dark blue | yellow | 3 | Dark blue | Pale orange | 4 | Blue | Pale orange | 5 | Pale blue | Pale orange | 6 | Blue | Pale orange | 7 | Pale blue | Pale orange | Discussion Part A In the separation and purification of a single protein or amino acids‚ a solution containing the desired analyte
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Electrophoresis of Amino Acids Introduction: Electrophoresis is a separation technique based on the movement of charged ions under the influence of an electrical field. This technique is primarily used for the separation of amino acids and peptides on the basis of their charge. All amino acids contain ionizable groups that cause the amino acids‚ in solution‚ to act as charged polyelectrolytes that can migrate in an electric field. The amino acids with a net positive charge will migrate toward
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Alanine (ala‚ A) (neutral‚ non-polar) Arginine (arg‚ R) (basic‚ polar) Asparagine (asn‚ N) (neutral‚ polar) Aspartic Acid (asp‚ D) (acidic‚ polar) Cysteine (cys‚ C) (neutral‚ slightly polar) Glutamic Acid (glu‚ E) (acidic‚ polar) Glutamine (gln‚ Q) (neutral‚ polar) Glycine (gly‚ G) (neutral‚ non-polar) Histidine (his‚ H) (basic‚ polar) Isoleucine (ile‚ I) (neutral‚ non-polar) Leucine (leu‚ L) (neutral‚ non-polar) Lysine (lys‚ K) (basic‚ polar) Methionine (met‚
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following double-stranded DNA sequence: 5’-CAG AAG AAA ATT AAC ATG TAA-3’ 3’-GTC TTC TTT TAA TTG TAC ATT-5’ If the bottom strand serves as the template‚ what is the mRNA sequence produced by transcription of this DNA sequence and Why? 5’-CAG AAG AAA AUU AAC AUG UAA-3’ mRNA sequence 3’-GTC TTC TTT TAA TTG TAC ATT-5’ DNA template strand We get the mRNA sequence due the transcription process‚ which gives us the RNA bases that are complementary to the DNA template strand that uses
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Amino Acids Amino acids are biologically organic compounds containing amine and carboxylic acid functional groups‚ usually along with a side-chain specific to each amino acid. The elements that are key of an amino acid are carbon‚ hydrogen‚ oxygen‚ and nitrogen. There are about 500 different kinds of amino acids found but we recognize 23 of the amino acids that are known‚ they are classified into three groups‚ essential semi-essential‚ and non-essential. Each amino acid has unique characteristics
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Characteristics and Properties of Amino Acids Introduction: Each amino acid has at least one amine and one acid functional group as the name implies. The different properties result from variations in the structures of different R groups. The R group is often referred to as the amino acid side chain. Amino acids have special common names‚ however‚ a three letter abbreviation for the name is used most of the time. A second abbreviation ‚ single letter‚ is used in long protein structures.Consult
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BEHAVIOUR OF AMINO ACIDS IN SOLUTION Abstract In this practical the acid-base behaviour of amino acids was assessed. Amino acids are amphoteric. They have the basic (amine) and the acidic (carboxylic) functional groups. These show the same type of equilibrium reactions that all weak acids and bases undergo‚ and the relative amount of each can be altered by adjusting the pH of the solution. For this practical glycine was used as a model to show this unique nature of amino acids. A series
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Experiment 1 Titration curves of amino acids General structure of amino acids (amphoteric type): Zwitter ion C * : α- carbon : α- amino acid NH2 : α- amino group‚ basic (proton acceptor) COOH : α- carboxylic group‚ acidic (proton donor) R : side chain of amino acid Classification of amino acids depending on the nature of side
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Amino acids are the building blocks of proteins. They band together in chains to form the stuff from which your life is born. Think of amino acids as Legos for your life. It’s a two-step process: Amino acids get together and form peptides or polypeptides. It is from these groupings that proteins are made. And there’s not just one kind of amino acid. A total of 20 different kinds of amino acids form proteins. The kinds of amino acids determine the shape of the proteins formed. Commonly recognized
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