Purification of Recombinant Green Fluorescent Protein (rGFP) From E. coli strain‚ BL21(DE3)‚ Using Ni2+-Agarose Affinity Chromatography Abstract: The purpose of these series of experiments was to express and purify recombinant Green Fluorescent Protein (rGFP) from the E. coli strain‚ BL21(DE3) by beginning with its purification via a Ni2+-agarose affinity chromatography column. The His6 tag of the rGFP bound to the Ni2+-agarose column and washes and elutions were obtained‚ with elution 3 containing
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on the number of stomata of a leaf.” Research Question: How do differing leaf heights affect the number/density of stomata of a leaf? Hypothesis Stomata are pores‚ typically found under the leaf (lower epidermis)‚ that control the gas exchange of transpiration‚ where water vapor leaves the plants‚ and carbon dioxide enters. I predict that the stomatal density on high leafs is higher than on low leafs. During photosynthesis the chloroplasts in the leaf cells synthesize ATP from ADP as
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GLIRICIDIA SEPIUM LEAF EXTRACT FOR HOGS MANGE INVESTIGATORY PROJECT IN SCIENCE SUBMITTED BY: CRISTEL GELIANNE M. ALOG SUBMITTED TO: MRS. LERMA A. AROSA INTRODUCTION A. Background of the study Gliricidia sepium is a kind of tree common in the community. People use it for fuel unaware of its value as medicinal plant. The idea that gliricidia
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Maple Leaf Foods Reaction Paper Introduction Canada is the country has the safest food system. However‚ the maple leaf food‚ one of the largest producers of meat products in North America and an integrated company with the value” We Take Care”‚ faced Listeria outbreak‚ described by the Company’s CEO as “the toughest situation we’ve faced in the 100 years of this company’s history”. The first part of this paper will give a general perspective on the actions and strategies of Maple leaf food in the
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Candy Chromatography: What makes those colors? The point of doing this project is to use paper chromatography to see which dyes are used in the coatings of your favorite colored candies. Chromatography is a technique for separating mixtures. Chromatography can be used to separate specific components from a complex mixture‚ based on molecular size or other chemical properties. It can also be used to identify chemicals‚ for example crime scene samples like blood‚ drugs‚ or explosive residue. Highly
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Encyclopædia Britannica Paper chromatography is used to separate "dissolved chemical substances". An example of dissolved chemical substances could be the pigments that make up a specific color‚ for example in a marker. When a chromatography paper experiment is done on a black marker‚ the experiment tends to separate all the different pigments that make up the color. In this experiment‚ the substance investigated was a black water soluble Giotto marker pen. Paper chromatography has two phases‚ the
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Supply chain of Betel Leaf: Preface: In perspective of supply chain of betel leaf‚ this paper has been showed the supply chain of betel leaf within the sylhet city. At the very beginning we discussed regarding betel leaf such as vernacular name‚ Bangladeshi’s betel leaf‚ area of cultivation‚ its varieties etc. So this part will show the Inventory process‚ Transportation‚ Location and Distribution process of betel leaf within the sylhet city. Description: The betel leaf is known as Paan in Assamese/Urdu/Hindi/Odia/Bengali
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many of us don’t want Mr. Poison Leaf to declare that the world’s salvation comes from whims‚ irrationality‚ and delusions. Still‚ we feel a prodigious pressure to smile‚ to be nice‚ and not to object to his batty criticisms. Mr. Leaf likes to argue that public opinion is a reliable indicator of what’s true and what isn’t. Even if there were a faint glimmer of truth in that argument‚ it would be extremely faint. The truth is that many people are worried that Mr. Leaf will use both overt and covert
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story “The Last Leaf” the false perception of this story has a powerful outcome. Sue led Johnsy to believe that the last leaf had not fell off from the vine. As Johnsy looked out of the window she could see the little leaf hanging on for dear life. Even through the snow‚ and the pouring down rain it stayed firmly attached to it’s vine. Johnsy counted as all the other leaves fell from it’s vine. But for some reason this little leaf held on strong. She began to wonder if this leaf was sending her a
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BEST AND FINEST LOOSE LEAF TEAS: Tea is the most important and popular beverages in recent days. To get a best cup of tea you have to use loose leaf teas which will add a better taste to your tea. Making of loose leaf tea is easy. It is very delicious to have loose leaf tea rather than tea bags. Loose leaf teas are made up of very large piece of leaves which adds a flavor to the tastes . All kinds of teas are produce from a plant which is known as Camellia sinensis but the variety of tea
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