unknown cultures were Gram stain‚ Catalase‚ Mannitol Salt Agar (MSA)‚ Blood Agar‚ Novobiocin‚ Coagulase‚ and DNAse (Alachi‚ 2007). Rebekah Worley February 21‚ 2012 Mitchell Section 4 Biol 311 Staining and Identifying Unknown Bacteria Introduction: The microbiology lab up to this point has been used to teach the students how to stain and identify bacteria. There are several types of staining through which the bacteria can be identified based on the color and shape. The staining methods used in the
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smear my bacteria on a liquid medium. I then proceeded to incubate the medium for 24-48 hours. 1. GRAM STAIN The next step I took in finding my unknown bacteria was to gram stain it. This is used to differentiate the bacteria. The different staining reagents are: crystal violet‚ grams iodine‚ acetone-alcohol‚ and grams safranin. Under the microscope it was a pink color‚ which means Gram Negative. Also‚ the shape was a rod. 2. KLIGER’S IRON
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The tests used to confirm the fermentation were a gas production test‚ pH test‚ Gram stain‚ and turbidity test. The results showed during the conversion of milk to Kefir there was gas produced‚ a decrease in pH‚ more bacteria present‚ and an increase in absorbency. The results proved that fermentation occurred with three positive confirmatory results from the gas production‚ pH‚ and turbidity tests‚ while the Gram stain test acted as a negative confirmatory test. Introduction: The main goal of
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slide before staining? Differentiate between a differential stain and a special stain (how are they different‚ don’t simply identify the types of differential stains and special stains). What is the role of the Gram’s iodine in a Gram stain? What color would all bacteria become at the end of the staining procedure if the iodine in the gram step was omitted? What is the role of the alcohol wash in the Gram Stain? What color would all bacteria become at the end of the staining procedure
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Introduction: The purpose of this lab is to use staining techniques and biochemical testing to identify an unknown bacteria using Bergey’s manual. Bergey’s manual of Systematic Bacteriology is a dichotomous key primarily used to identify a bacterial species. Biochemical tests are used to differentiate different species of bacteria. These tests are effective in determining the characteristics of the microbe being tested. Such characteristics include citrate utilization‚ gelatin hydrolysis‚ nitrate
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determine the identity of unknown number 34‚ a gram stain‚ citrate utilization test‚ and a urease detection test. These tests are explained in detail‚ and the method of performing the tests is described. The tests showed that the bacterium was gram-negative bacilli that did not utilize citrate‚ and the ability to hydrolyze urea using urease. These results indicate that the identity of unknown number 34 is Proteus mirabilis. Introduction Proteus mirabilis is a gram-negative bacillus bacterium. It is found
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physiological class of bacteria capable of utilising agar as a sole carbon source. This ability is made available by the use of agarases - enzymes which break down agarose into oligosaccharides. This physiological class branches through genii‚ regardless of Gram Stain status or morphology. Through a review of scientific literature we can find identification methods‚ optimum conditions and the general function and location of agarolytic bacteria‚ as well as methods to culture them in vitro for study and experimentation
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Activities/Tasks/Responsibilities | Problem(s) Encountered | Action(s) Taken | Personal Reflection | BacteriologyJuly 4- July 31 | Receiving of specimen‚ Logging‚ Processing of specimen‚ Inoculation into media‚ biochemical testing‚ identification of organism‚ gram staining‚ AFB staining‚ releasing of results‚ culture sensitivity testing. | No major problems were encountered during my rotation for Bacteriology. Minor problems like unfamiliarization of SOP’s and other procedures are encountered. | Asking proper procedures
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results‚ the isolated bacteria were tentatively identified as Agrobacterium strains. B.Characterization of Agrobacterium tumefaciens: Biochemical tests: biochemical features of the selected isolates were given in the table 1. The Gram reaction indicates selected isolated are Gram negative. All the six isolated are positive
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Pure Culture Techniques In this first lab‚ you will be learning some very fundamental and important techniques. As is the case with most things‚ shorts cuts usually get you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques:
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