pathogens and their related epidemiology has become increasingly more important. The purpose of this study was to identify an unknown bacterium in a controlled laboratory environment over a 5 week period. Utilizing a variety of differential testing and staining methods learned in the microbiology course‚ students were to determine the identity of an assigned unknown organism. Observations were made and recorded each week to narrow down the scope of identification. Data has been presented in the tables
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the plate‚ it could be seen that the unknown had growth and formation of colonies. The Gram stain was chosen next to determine if the unknown was gram-negative or gram-positive. After looking at the gram stain under the microscope with the oil immersion it could be determined that the unknown was gram-negative due to the fact that the bacteria was pink in color and rod shaped. After determining the unknown was gram-negative‚ other bacteria’s were able to be eliminated from the options. The Triple Sugar
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particular order. Name this Procedure Identify the two types of bacteria present by shape and gram stain. In a gram stain what is the primary stain? The mordant? The counterstain? How does this differ from a simple stain? Identify the shape and gram stain of the bacteria present in this gram stain. Identify the shape and gram stain of the bacteria pictured. Think on this one! Name the gram stain and shape of the bacteria pictured. Name the genus of the organism pictured. Note the size
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Abstract: It is important to be able to identify pathogenic bacteria that may be causing harm. Tomato crops can be affected by several different pathogenic bacteria. By using Koch’s postulates‚ it was determined that Pseudomonas syringae was the bacteria causing rot. There are four criteria that must be met when using Koch’s postulates. They are that the organism must be fund in all infected‚ the organism must be isolated in pure culture then once reinnoculated in a healthy host‚ must cause the
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Having the ability to collect and identify unknown microorganisms is vital in health and medicine. This capability is important for a variety of reasons‚ such as knowing the causative agent of disease‚ knowing if the microorganism obtains any beneficial properties and knowing the correct microorganism to use to create a successful antibiotic. Implementing the experimental methods learned thus far in the microbiology laboratory allowed an unknown bacterium to be identified as a result of this study
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Bacterial Smears Are Fixed before Staining to? Answer It is important to heat fix the bacterial smear before staining so as to‚ kill the bacteria‚ firmly adhere the smear on to the microscopic slide to prevent washing off during staining‚ and to allow the sample to readily take up the stain. Reference: www2.hendrix.edu What is the purpose of heat- fixing the smear? It helps the cells adhere to the slide so that they can be stained. The purpose of heat fixing is to kill the organisms without
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MacConkey Agar test This test is used for isolating the lactose fermenting bacteria from lactose non-fermenting bacteria while inhibiting the growth of gram positive bacteria. The lack of growth from my bacteria indicates that my bacterium has negative result. Mannitol Salt Agar This test is used to observe the difference between gram positive staphylococci bacteria and to differentiate for mannitol fermentation. If a yellow media change is observed then the bacteria has the presence of
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In March 10‚ the first procedure that was done was gram staining. The purpose of this procedure was to determine if the unknown bacterium is gram-positive or gram-negative‚ and also to determine the cell shape. Gram-positive cells will be purple while gram-negative cells will be pink. The methods for the Gram staining method first starts with a fixed smear of the bacterium‚ covered it with crystal violet for 30 seconds. Afterwards‚ it should be gently rinsed off with distilled water and be covered
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Microbiology Laboratory Report Identification of Unknown Bacteria 6O BIO 251-7H1 Gram Stain Summary: Out of 28 Unknown microorganisms‚ slant 6O was randomly selected‚ and subjected to testing in order to identify it’s species. Seventeen of the unknown bacteria were classified as Gram-negative bacilli: Corynebacterium pseudodiphtheriticum‚ Enterobacter aerogenes‚ Eschirichia coli‚ Flavobacter capsulatum‚ Helicobacter pylori‚ Klebsiella pneumonia‚ Klebsiella oxytoca‚ Proteus hauseri‚ Proteus
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Review Sheet Exercise I: Survey of Higher Microorganisms: Protozoa‚ Fungi‚ and Helminths Protozoa (group of Kingdom Protista) 1. Amoeba a. nucleus- dark center of the cell b. food vacuole- They feed by taking nutrients into the cell by diffusion and packaging it into (clear circles spread throughout the cell) c. pseudopod- “false foot”; the motility results from the streaming of the protoplasm that forms the process 2. Entamoeba causes amoebiasis or amoebic dysentery‚
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