shapes would distort. BM SC Negative Stain CULTURES: Bacillus subtilus Staphylococcus epidermis MATERIALS: Nigrosin‚ Clean slide‚ Distilled water‚ Sterile toothpicks FUNCTION: This technique is very useful in situations where other staining techniques do not show clear morphology or size. PROCEDURE: 1. Place a small drop of nigrosin near on end of the slide. Mix a loopful of broth culture in the drop. (When from a solid medium‚ add a loopful of water) 2. Draw a second slide
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Pets as carriers of soil inhabitant microbes into our homes GIZELL VASQUEZ Biology department‚ Pathogenic microbiology‚ Crosby Jones‚ Angelo State University‚ San Angelo‚ TX 76909 There are approximately 78.2 million owned dogs in the United States (1)‚ and according to the United States government the current population of the nation is 308.7 million. The portion of dogs to humans is 4:1. These statistics are of importance because these pets physiologically observed their environment by smell
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is a practice that aids in Increasing ones knowledge of a laboratory setting and it helps improve on Using sterile technique. Isolates of soil microbes can be categorized and Characterized based on a number of criteria ranging from gram-staining Which is done for this project to enumeration which is quantitative description Based on the amount of microbial colonies available. The Antimicrobial Activity of three different microbes were also tested and results were obtained
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appearance was observed and recorded. There was growth that appeared pinkish in color. The first test we did was basic stain using a heat fixed emulsion which kills the bacteria and allows them to adhere to the slide and thickens their protein for better staining. I then covered the heat fixed emulsion with crystal violet for one minute. The stain was then rinsed with distilled water. The stain was then pat dry with bibulous paper then observed under oil immersion to determine morphology. Upon completion
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Trypticase Soy Agar plate to isolate individual colonies to be studied‚ tested and identified. After incubation of the individual TSA plates‚ the morphologies were viewed and noted and a Gram stain was completed on each individual bacterium‚ which will be referred to as Bacteria #1 and Bacteria #2. After the Gram reaction was determined on Bacteria #1 and Bacteria #2‚ different biochemical tests were done according to the dichotomous keys provided in the lab manual. All the tests were performed by
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purpose of this lab is to: 1. Collect a sample of soil from (your school) on October 27th 2011 and collect a sample of pond water from (the name of your lake or pond) on October 27th and record the data results for both samples. 2. Using the Gram stain‚ and light microscope identify at least 2 Prokaryotes (bacteria) found in the water samples that are isolated on the MacConkey agar plates and the nutrient agar plate. Using the Identification Lab manual‚ identify at least 2 Eukaryotes (fungus)
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* Length of L. lactis ranges from 0.5-1.5 micrometer (µm). * L. lactis does not produce spores (nonsporulating). * No capsule formation. * L. lactis grow in microaerophilic conditions * L. lactis are non motile. * L. lactis are Gram (+ive). * L. lactis are catalase (-ive). * L. lactis have low-GC content. * L. lactis are acid-tolerant * Optimum growth temperature is 30oC at pH 6.8. * L. lactis are not produce CO2 in MRS Broth. Colony Morphology: * No
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microbiology lab techniques including an API test‚ an oxidase test‚ a gram stain‚ a hanging drop slide‚ and morphology identification. The unknown bacterium‚ which was contaminated with Serratia marcescens‚ was isolated by streaking the bacteria solution to single colonies. The isolated unknown white bacteria‚ had the appearance of circular form‚ convex elevation‚ entire margin‚ elongated cocci. The tests than showed that the bacteria was gram-negative‚ non-motile‚ and was identified as a Salmonella species
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Review for Microbiology Test #1 (Lesson 1-5) Lesson 1 What is the #1 killer of man worldwide? Heart Disease. What is the #3 killer in the US? Infectious Disease. What is the importance of MO in our world? List 6-8 reasons MO are important. Can’t live in Germ Free World‚ Keeps Economy running‚ Agriculture‚ Medication‚ Baking‚ Cosmetics‚ Paints‚ Fertilizers‚ Helps develop immune system‚ Decomposition of dead plants and animals to enrich the soil. What are the 4 groups of people most prone to
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Unknown Lab Report Unknown Organism #6 Ann Le (Phuoc) May 6‚ 2010 Dr. Carrington Microbiology Lab- MW 12:50 Le 1 I. Introduction My unknown organism #6 is Morganella morganii‚ which is a gram-negative bacillus rods commonly found in the environment and also in the intestinal tracts of humans‚ mammals‚ and reptiles as a normal flora. (3‚ 5) This bacterium Morganella morganii‚ was first discovered in the 1906 by a British bacteriologist named H. de R. Morgan. (2) Despite its wide
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