sample was taken from a door handle to the George Lynn Cross Hall‚ which is touched by hundreds of students entering the building every day. The most important exercises performed include gram staining‚ which
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was correct. The findings are that we were able to identify‚ by process of elimination‚ the four different test bacteria. PROCEDURE - These procedures were followed for all four media test samples. Label test sample. Record Case study and Gram Stain results. For glucose testing: Select red glucose broth with durem tube. Inoculate red glucose media. Incubate for 24 hours. Record results. For lactose testing: Select red lactose broth. Inoculate red lactose media. Incubate for 24 hours
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by Bacillus sp.: The microorganisms in the most contaminated toilet Sink were isolated by dilution technique. The dominant bacterial strains are identified as Bacillus sp.‚ on the basis of morphological and biochemical characteristics. Gram’s staining: Gram positive and rod shape. They were serially diluted and spread on nutrient agar plates. The starch has been hydrolyzed and a clear zone by the addition of iodine solution. Visual identification: When bacterial biomass mixed with silver nitrate
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report discusses an experiment that was done to demonstrate the effects of lysozyme on populations of Gram positive and Gram negative bacteria. Bacteria have a cell wall composed of peptidoglycan that gives the wall its strength. Gram negative bacteria have and extra component of lipopolysaccharide (LPS)‚ that is stabilized with magnesium ions‚ to their cell wall that further protects them. When Gram positive bacteria are treated with lysozyme‚ the lysozyme breaks down the peptidoglycan‚ allowing the
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and was moved to the incubator for 48 hours at 35 degrees Celsius. The same procedure was used on all of the samples. Following the 48 hour incubation period‚ the Petri plates were observed for colony morphology‚ and the results were recorded. A Gram
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is to distinguish and indentify an unknown bacterium. There are several tests that can help one eliminate and narrow down the options. The most useful test‚ and the very first one done‚ is a gram stain. This test will tell whether the bacterium is gram-positive or gram-negative. After the type of gram stain is identified‚ the tester has a wide array of differentiating tests at their disposal. Based on the results from these tests‚ and the numerous others that are available‚ one can accurately
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I was able to come to the conclusion that my unknown is Klebsiella pneumoniae through the use of Bergey’s Manual. After performing a gram stain of my unknown I was able to determine that my unknown was a gram-negative rod. While testing the oxygen requirements my test tube had growth throughout the tube‚ indicating my unknown was a facultative anaerobe‚ which can grow in the presence or absence of oxygen. The most important test was whether my unknown was motile or nonmotile. My test tube only showed
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isolate the unknown." It is also customary to write in the past tense for most of the report. This includes the introduction‚ the summary‚ the description of the materials and methods and the results. The present tense is reserved for the conclusions about the results. See the examples given below. Some other general rules that should be followed are: Microbial nomenclature: The name of the bacterium should written and spelled correctly. The name should be italicized or underlined. Italicized
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Since it was gram positive‚ I knew my organism had a thick peptidoglycan and no outer membrane. After finding out my organism B was gram positive‚ I did the catalase test on it. After adding the two drops of H202 and seeing bubbles‚ I ruled out Streptococcus lactis. My organism did have the catalase enzyme; meaning it was
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methods that have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium. II. Results Unknown A a. The unknown is a gram negative bacilli Upon performing a gram stain on a colony from a streak of the unknown bacteria‚ at 100x‚ the bacteria stains negative for the gram stain. In addition‚ the structure of the bacteria is bacilli. b. The unknown is a facultative anaerobe A small amount of bacteria was added to a tube of fluid thioglygollate
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