Unable to read the Gram Stain at first it was later confirmed that Unknown #7 was a gram negative bacteria. When introduced to Starch Unknown #7 produced no zone of clearing showing that it did not contain the enzyme amylase. When introduced to the Lipid Unknown #7 did produce a zone of clearing indicating that it did contain the enzyme lipase. When introduced to Casein Unknown #7 produced no zone of clearing indicating that it did not contain the enzyme protease. When streaked onto the Gelatin‚
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Unknown Organism Report Unknown Organism # 4 Klebsiella pneumonia Klebsiella pneumonia has a rod gram stain with the agar slant cultural characteristics being slimy‚ white‚ somewhat translucent‚ raised growth. It is small gram-negative coccobacilli that can cause lobar pneumonia. The litmus milk reaction is acid‚ gas‚ and curd with a variable reaction. Klebsiella can grow either in the presence or absence of oxygen because of its nature of being a facultative anaerobe. Oxygen is a preferred energy
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(2013) reported a method for isolation of E. coli O157 from meat sample. Twenty five grams of meat sample was blended with 225 ml of sterile modified tryptone soya broth containing vancomycin (40 μg/ml) followed by incubation at 37 °C for 18 h. Enriched culture was plated onto sorbitol MacConkey agar supplemented with cefixime and potassium
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was in our number seven vial would become a battle we were willing to take on. As I began the test of deciding if our little bacteria friend was gram positive or negative‚ Jordan my science teammate‚ was putting together a smear plate. In as little as ten minutes we had discovered by the pink oval shapes we were observing‚ our microbe friend was a gram negative rod. We had narrowed our search down to five! We decided next we would do oxygen along with a motility test. Along with those‚ we did a fermentation
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As a result of the tests ran‚ I was able to identify the unknown bacteria. For Unknown A‚ I ran six tests. I first isolated the bacterium from the second bacterium and found a clear growth (Table 1‚ Figure 1). Secondly‚ I ran a gram stain and found a gram positive‚ cocci bacterium (Table 1‚ Figure 2). Third‚ I ran a catalase test in which was negative (Table 1). From here‚ I determined a starch hydrolysis test would be necessary to distinguish between different bacteria. The result was negative (Table
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with previously in lab. Initially a Gram stain was conducted in order to distinguish the unknown bacterium as a Gram-positive and/or a Gram-negative organism (Lancaster and Bennett‚ 2012; Kellenberger‚ 2001). Based upon the results‚ both Gram-negative and Gram-positive bacteria were observed in the unknown mixed culture (Table 1 and Table 2; Kellenberger‚ 2001). In order to isolate the two different bacteria‚ colonies that grew on the MSA were used to inoculate Gram-positive tests‚ where as MacConkey
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color. E. coil on the EMB appears metallic green and P. aeruginosa appears dark purple on the agar. E. coli turns green because of the sugar lactose. EMB is differential media because of the green color of E. coil and it also selects for gram negative and inhibits gram positive. TSA plate is clear and has white color. E. coli grows best on Mannitol plate‚ E. averogenes grows best on the EMB‚ and finally E.coli also grows best on the TSA as well. The results for the known organisms are fantastic and they
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culture was performed on a Tryptic Soy Agar Plate for isolation and purity and then incubated at 37 degrees Celsius for 24 hours. A gram stain was performed to determine whether the bacterium was a gram negative or a gram positive. After performing the gram stain‚ I concluded that by the appearance of purple spherical clusters resembling grapes that is was a gram positive cocci. A Catalase test was then performed using hydrogen peroxide. A positive catalase test was observed when bubbles started
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Gram Negative Unknown Lab Report Abstract The Unknown Gram Negative bacterium inoculated in a Tryptic Soy broth medium was randomly selected from a group of other unknowns. In order to identify this unknown the seven different types of biochemical tests will be conducted on this unknown bacterium to identify it out of 6 possible bacteria; Escherichia coli(E. coli)‚ Enterobacter aerogenes (E.aerogenes)‚ Klebsiella pneumoniae (K.pneumoniae)‚ Proteus mirabilis(P.miranilis)‚ Pseudomonas aeruginosa(P
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conducted on unknown bacteria 32 was the Gram stain. From this stain‚ unknown 32 was found to be a Gram-positive cocci. This test eliminated all possible Gram-negative bacteria‚ Gram-positive rods and Gram-positive spirillium. Next‚ the endospore test determined whether or not the Gram-positive bacteria contained endospores. With the use of malachite green‚ steam‚ and safranin it was found that unknown bacteria 32 did not contain endospores. This eliminated Gram-positive cocci Sporosarcina spp. The oxidase
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