1. Make sure you label both plates on the bottom‚ with your name‚ date and name of pathogen you have chosen (listed above under materials) Then label numbers 1-5 on one plate and 6-10 on the other as there are 10 disks that need to be placed. Spread out in a circle formation like pictured below (This is the final product; look at this for reference on how to label) Now you are ready to start culturing. Start with one of your plates you can do both at the same time but to avoid making any errors
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Lakeli is absolutely stunning‚ but for his beauty he has paid the price. He is a beautiful 12 week old deaf Great Dane. While we all think white Great Danes are stunning most of them are going to be either blind‚ deaf‚ and occasionally both. What is a Double Merle and how are they created? A few things to keep in mind about the Merle pattern as you read. •Merle is a pattern not a color. •Merle is a bleaching pattern. •The Merle gene creates mottled patches of color in a solid color coat‚
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The wild type and the mutant type strains of Halobacteria were used in this experiment to examine the phenotypic differences against each strain’s genotypes. The mutant strain (KBT-1) did not possess gas vesicles‚ which decreased its ability to float to the surface. The lack of gas vesicles in the mutant strain made the colonies a red color. The wild type strain (NRC-1) had gas vesicles and appeared a pink pigment color. Growth on the agar allowed one to examine the specific colonies. Inoculation
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ASSAYING PLANT SUBSTANCES FOR ANTIBACTERIAL ACTIVITY PURPOSE To test plant extracts to see if they possess antibacterial activity. PROCEDURE First create a list of plant extracts being tested and choose 2 extracts per group. The plants being tested are Oregano‚ Tea tree‚ Eucalyptus and Colloidal Silver. Obtain two nutrient agar plates and using a permanent marker draw four quadrants on the bottom of each agar plate. Using a sterile pipet transfer 250 ml of E. coli broth to the middle of
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Jody Kaur Pure Cultures Lab 1/31/13 Introduction : Pure cultures are made of only one type of organisms and can be used to study their properties. A method used to isolate pure cultures is making a steak-plate‚ which is a dilution process in which culture is spread over an agar plate in a certain manner. Using a loop rod‚ culture was taken from the tube and dragged across area 1 several time‚of the agar. The agar was then turned 90º‚ and the loop was flamed and cooled. Taking some culture
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Introduction The Gram stain is one of the most useful staining procedures in microbiology . It is one of three differential staining techniques‚ is used to identify divide bacteria into two groups: Gram-positive bacteria and gram-negative (Madigan‚ Martinko‚ Dunlap‚ Clark (2009). These staining reactions take advantage of the fact that cells or structures within cells display dissimilar staining reactions that can be distinguished by the use of different dyes (Brown 2009). A gram positive bacteria
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1. Describe the major differences between gram positive and gram negative bacteria cell walls. The gram negative bacteria cell wall is a thin peptidoglycan layer and an outer cell membrane with a lipopolysaccharide layer. The gram positive bacteria cell wall is a single thick peptidoglycan layer. This wall forms in a mesh like formation of three layers of alternating material. 2. From the procedure that you have carried out‚do you feel that the Gram positive stain is a simple procedure? No‚
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hyphal cells. d. True hyphae- Fundamental microscopic units of fungus‚ tube-like projections with no constictions at the cell wall. The cell walls remain parallel with no indentation. 3. Describe the appropriate specimen collection procedures‚ staining methods‚ and culture techniques used for isolation of yeast. Collection procedures Specimen of choice include respiratory secretions‚ hair‚ skin‚ nails‚ tissue blood or bone marrow and CSF. Swabs are inadequate. CSF: Concentrate by centrifugation
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Differential Staining Purpose: The purpose of this experiment was to become familiar with subtypes of culture media and the uses for each‚ learn and employ the streak and pour dish techniques‚ and generate a pure culture of a specific organism. Set Up: For this experiment I needed: 1 Distilled water‚ 1 Paper towels‚ 1 10%-bleach or 70% alcohol solution‚ 1 Zip bag‚ 1 Pan to heat agar‚ 1 Isopropyl alcohol (rubbing alcohol)‚ 1 Cultures: S. epidermidis and L. acidophilus‚ 1 Gloves‚ Disposable
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Theories of Staining Techniques Staining bacteria with different dyes via staining techniques‚ allows in distinguishing the microorganism from its backgrounds. Also‚ helps in studying different internal structures such as vacuoles‚ cell walls and spores in details (Seeley and others 1991). Some staining techniques such as Gram staining‚ endospore stain and capsule stain are some of the theories of stains used in bacteriology today. Also‚ these staining procedures help in determining properties
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