Introduction:The objective throughout the gram staining experiment was to classify bacteria through their chemical nature as well as the physical structure of their cell walls. Through this experiment one would be able to observe either a thick or thin layer of peptidoglycan by the appearance of the bacteria staining either gram-positive blue-purple or gram-negative pink. Question: Is there a subsequent effect of the bacteria when decolorizing with alcohol? Hypothesis: If there is either a removal
Premium Bacteria Microbiology Chemistry
Introduction Gram staining was developed by Christian Gram in the 1800’s‚ a Danish bacteriologist. (Smith and Hussey‚ 2005) It was the first differential staining technique and most common used in microbiology. Furthermore‚ bacteria are transparent and cannot be seen through the microscope. For that reason‚ Gram staining is an important tool for distinguishing between two main types of bacteria Gram-positive and Gram-negative. The Gram stain differentiates the Gram positive and gram-negative on the
Premium Bacteria Microbiology Escherichia coli
Gram Staining Lab Introduction Gram staining is a very important technique used in biology labs all over the world. It is a technique used to differentiate types of bacteria using certain physical and chemical characteristics of their cell walls. Gram positive bacteria (which show up purple after the gram staining process) have a very thick layer of peptidoglycan where gram negative bacteria (which show up pink after the gram staining process) have a much thinner layer of peptidoglycan. One thing
Premium Staining Gram staining Bacteria
Bacteria Classification By Gram Staining THE AMERICAN UNIVERSITY IN CAIRO BIOLOGY DEPARTMENT SCIENCE 453 : BIOLOGY FOR ENGINEERS REPORT No.1 Presented By : Karim A. Zaklama 92-1509 Sci. 453-01 24/2/96 Objective: To test a sample of laboratory prepared bacteria and categorise it according to Christian’s gram positive and gram negative classes and also by viewing it under a high powered microscope and oil immersions; classify its shape and note any special characteristics. Introduction: Bacteria
Premium Staining Gram staining Bacteria
multiple techniques for identifying bacteria. Learning how to gram stain‚ use specific media such as MacConkey agar‚ and test antibiotics to see which antibiotic would react best against a specific organism. All these techniques helped me prepare for the final lab‚ identification of an unknown bacterium. For the final lab‚ I received the organism “6A”. To start identifying this organism‚ I did a gram-stain to identify if the organism is gram positive or negative. I created two slides to ensure that I
Premium Bacteria Microbiology Gram staining
to isolate the unknown." It is also customary to write in the past tense for most of the report. This includes the introduction‚ the summary‚ the description of the materials and methods and the results. The present tense is reserved for the conclusions about the results. See the examples given below. Some other general rules that should be followed are: Microbial nomenclature: The name of the bacterium should written and spelled correctly. The name should be italicized or underlined. Italicized
Premium Gram staining Present tense Staphylococcus aureus
Stainin g What is Cellular Staining? Cell staining is a technique that can be used to better visualize cells and cell components under a microscope. By using different stains‚ one can preferentially stain certain cell components‚ such as a nucleus or a cell wall‚ or the entire cell. Most stains can be used on fixed‚ or non-living cells‚ while only some can be used on living cells; some stains can be used on either living is or non-living What Cellularcells. Staining? The most basic reason that cells
Premium Staining Bacteria Microscope
Isolation of Pure Cultures by Dilution Techniques and Gram Staining Method Results Table 1. Gram stain reaction and cellular features of the culture. Gram staining methods were applied on the given mixture of Bacillus cereus‚ Escherichia coli and Staphylococcus aureus and then examined microscopically. Results were recorded in Table 1. Gram Reactions Cell shapes Cell Ends and Arrangement Size Distinctive Characters Predicted Bacteria Bacteria 1 Gram positive (purple) Cocci Rounded‚ clusters‚ singly
Premium Gram staining Bacteria Escherichia coli
MBK – Lab Report Differential Staining Questions: A. What is a differential stain? How is it different from a simple stain? 1. A differential stain is a stain that differentiates between gram-positive and gram-negative bacteria. Bacteria that stain purple are considered gram-positive and those that stain pink are considered gram-negative. 2. A simple stain is a stain that reacts with all microbes in and identical fashion. They are used to increase contrast. 3. They
Free Bacteria Staining Gram staining
Differential Staining Cynthia Alonzo‚ M.S. Version 42-0242-00-01 Lab RepoRt assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions‚ diagrams if needed‚ and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information
Premium Gram staining Staining Bacteria