(2005) defined staining as the method of artificially producing color in microorganisms to allow for the visualization under the microscope. He further stated that stains are used not only to make the organisms visible but also to understand their structure and chemical nature. Gram staining is a technique commonly used in the laboratory. It is used to differentiate two large groups of bacteria based on their different cell wall constituents. It distinguishes between Gram positive and Gram negative groups
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MICROBIOLOGY 3444-007 Gram Negative Unknown Unknown Bacteria #14 LeNaiya Kydd 4/2/2014 Abstract In order to be able to identify the unknown organism that was given to us‚ we had to conduct a number of different tests. These biological tests are used because they help us be able to identify the properties of the unknown we have and be able to compare our observed results with actual results of all the potential organisms. When all of the data of the test are put together it is easy to
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Bio Lab Report Title: Gram-Negative and Gram-Positive contamination in yogurt. Introduction: There is bacteria that is beneficial and harmful in the human. Yogurt was choosed to be the best example that contains this bacteria. What happens to be the difference between these two types of bacteria‚ and how are they harmful? The bacteria is considered “Gram-Negative‚” and the substance is considered contaminated when there is more negative bacteria than positive bacteria. This will prove or disapprove
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Momordica charantia L. Extract as a Prophylactic Medicine for Gram-Negative Bacteria Escherichia coli Proponents: John Rovic P. Ngo Lester T. Almarez Trisha Mae N. Reyes August 2013 In Partial Fullfillment of the Requirements in Research II-B Momordica charantia L. Extract as a Prophylactic Medicine for Gram-Negative Bacteria Escherichia coli A Science Research Project Presented to The Faculty of Science and Technology Mr.June Hayden Sinson Research Adviser
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The Comparative Analysis of Simple Staining and Gram Staining Techniques by observing E. Coli and S. Pyogenes under the Compound Light Microscope INTRODUCTION: A German bacteriologist‚ Dr. Theodore von Escherich‚ was the first man in 1885 who discovered the bacterium named Escherichia coli‚ which are gram negative and appears in rod shaped. Most kind of bacteria E. Coli does not cause diseases and some strains indeed are beneficial in helping the process of food breaking down in the intestines
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different cultures were observed. In order to proceed identification‚ those cultures were isolated as separate organisms‚ using inoculating streak technique and incubated until the next class period. The expected result was supposed to show Gram positive and Gram negative bacterium growing on a separate media. Having checked two Nutrient plates‚ signs of visible growth of two different bacterium were present. Due to the fact‚ that one type of bacteria didn’t appear to be fully
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1.What is the purpose of staining samples?A) To increase the contrast and visibility of the specimen B) To kill the specimen C) To see the specimen without the aid of a microscope D) To make the specimen appear larger in the microscope2.Which of the following are bacterial SHAPES? (Select all that are correct. Be sure to select the appropriate scientific name. You lose points for choosing incorrectly or for not choosing a correct choice.)A) Vibrio B) Strepto C) Spirillum D) Staphylo E) Cocci
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Exercise 8-B Differential Staining Gram Staining and Acid Fast Staining Introduction: Differential Staining‚ one which facilitates differentiation of various elements in a specimen is a general term that can refer to a number of specific processes. Using multiple stains can better differentiate between different microorganisms or cellular components of a single organism. Gram’s Stain is a widely used method of staining bacteria as an aid to their identification. It was originally devised by Hans
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LAB REPORT NUMBER TWO DATE: 3/25/2010 inal attachment Lab Experiment number 11 PURPOSE: To learn the Gram stain technique‚ the reason for the stain‚ and how to identify the results of the organisms stained. MATERIALS: Bunsen burner‚ inoculating loop‚ staining tray‚ glass slides‚ bibulous paper‚ lens paper‚ oil‚ and microscope METHODS: Apply Crystal Violet (Primary stain) for 1 minute. Rinse with D-water Apply Iodine (Mordant) for 1 minute. Rinse with D-water. Apply Alcohol (Decolorize) for
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ASEPTIC TECHNIQUES AND CELL COUNTING INTRODUCTION:- Microbes are single celled organisms and are so small that we cannot see with our naked eyes‚ microbes can only seen under microscopes .Microbes are the one of the oldest living form on earth‚ fossils of microbes were found which are said to be 3.5 billion years old . Microbe world: what is a microbe [online].[Accessed 9 April 2013].Available from: world wide web:‚microbes are found everywhere in rocks‚air‚water some of the microbes are even
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