investigations of each unknown in Table 1 by completing the following steps: 1) Apply the stain to your first unknown slide and examine it under the microscope. 2) Record the shape of the bacteria‚ the arrangement of the bacteria‚ and the gram staining characteristics. 3) Analyze and record the G+C content of the sample by dragging the DNA tube that corresponds to the bacterial sample to the GC Content Measuring Apparatus. (Note: the identification of the DNA tubes may be confusing;
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any two (2) microorganisms that are not classified as prokaryotes are and The two (2) genera of bacteria that are capable of producing spores are and b) c) d) An example of a Gram positive bacteria is example of a Gram negative bacteria. and is an e) Three (3) common differential staining methods used in microbiology are ‚ and "Ubiquitous" in microbiology means The minimum inhibitory concentration (MIC) means Of more than three thousand (3000) species of bacteria‚ only 10%
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Microbiology Lecture Exam #1 Outline Chapter 1: Microbiology Introduction I. Microbiology Definition II. History of Microbiology (Christian Gram‚ Robert Koch‚ Antony van Leeuwenhoek‚ Robert Hook‚ Edward Jenner‚ Louis Pasteur‚ Elie Metchnikoff‚ Dmitri Iwanowski‚ Paul Ehrlich‚ Alexander Fleming) II. Scope of Microbiology A. Infectious Agents 1. Prions 2. Viroids 3. Viruses B. Prokaryotes
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ferment the product can be stained with the gram staining. Gram staining is the method of differentiating bacteria into 2 large groups of Gram-positive bacteria and Gram-negative bacteria based on their cell wall. Most of the bacteria or yeast in the starter cultures used are Gram-positive. Gram staining requires 4 types of solutions used in the procedure: crystal violet‚ mordant‚ alcohol and safranin. The Gram-positive microbes will have blue stain while Gram-negative stains pink after the procedure
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4.0 Result 4 5.0 Discussion 4 6.0 Conclusion 5 References 6 1.0 Summary Mastitis
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_Control_______ variable. Through the semester‚ you have used two bacteria that produce colorful colonies: one produce yellow colonies‚ another one pink. What are the names of these two bacteria? Are they Gram+ or Gram-? Micrococcus Luteus‚ yellow colonies‚ gram + Serratia Marcescens‚ pink colonies‚ gram - There was one bacterial
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followed‚ they provide accurate data. 2. In 1884‚ Hans Christian Gram described a method of staining bacterial cells while not staining surrounding animal tissues; however‚ he thought the staining method he developed was faulty because not all bacteria stained. In a letter to the editor of the journal in which Gram published his findings‚ write your response to Gram’s concern. 3. Compare and contrast gram-positive and gram-negative cell walls with regard to (a) sensitivity to antimicrobial
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and evident in rat bite fever Exercise 3: Gram stain What is the function of the iodine solution in the Gram stain? If it were omitted‚ how would staining results be affected? Omitting the iodine in the gram’s stain would mean‚ the crystal violet that goes into the cell would be incapable of forming the Crystal Violet Iodine (CVI) composite. The CVI complex molecule possesses a bigger size than that of Crystal violet molecule; therefore‚ gram-positive bacteria are able to maintain the stain
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This particular type of agar only allows for the growth of gram negative rods. In performing this particular test‚ distinguishing factors between lactose fermenters and non-lactose fermenters can be shown. If the organism applied to the agar produces orange colonies‚ and if the area around the bacteria turns orange
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antibiotic that works on gram negative bacteria. Gram-negative bacteria are bacteria that do not retain crystal violet dye in the Gram staining protocol whereas Gram-positive bacteria are those that are stained dark blue or violet by Gram staining. The bacteria we used were E-coli that are a gram negative bacteria and the only antibiotic out of Penicillin and Streptomycin that will work is Streptomycin. Penicillin is also effective antibiotic but it will only work on bacteria that Is gram positive because
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