Robert Suran May 1 2013 Microbiology Abstract Over a three week time span I was able to successfully identify an unknown microorganism by combining the results of several tests. The first test was the Gram stain test which showed the microorganism was Gram positive and rod shaped. Using an Unknown Identification Flowchart‚ I proceeded to inoculate a Starch plate. In week 2‚ I analyzed the Starch plate and was able to determine that the results for Starch Hydrolysis were negative
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My partner and I were given unknown number 3 in the laboratory. After performing various tests over the course of a few weeks on our unknown‚ we came to the conclusion that our unknown organism is Klebsiella pneumoniae (K. pneumoniae). K. pneumoniae is a gram negative bacillus shaped microorganism. We observed that K. pneumoniae is a nonmotile organism. We performed multiple tests on our unknown culture‚ therefore we are very confident that it is correctly identified. We identified that K. pneumoniae
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Microbiology report Gram stain Objectives: The aim of this experiment was to be able to stain bacteria correctly and be able to see it under the light microscope. In 1883 Hans Christian Gram discovered an important staining method that is used extensively today. The stain is called the Gram Stain. This experiment was done in order to differentiate microbes into two basic groups: Gram positive microbes and Gram negative microbes. The purpose of this experiment was to learn the gram staining method and to
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MICROBIOLOGY SECTION The microbiology section aims at studying yu ailments and diseases from the isolation of suspected causative organisms. The processes involved in the isolation of these organisms include; culturing‚ staining‚ microscopy and sensitivity tests. Samples collected for examination include; stool‚ blood‚ sputum‚ urine‚ vaginal swab‚ wound swab and wound biopsy. Bacterial Culturing and sensitivity Cultures are carried out to isolate suspected organisms from a sample. There are different
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Microbiology Lab Quiz #2 Study Guide Exercise 9 -- The Gram Stain Compare and contrast simple and differential staining techniques. Simple Staining Simple staining is useful in determining the basic morphology of an organism. Simple staining involves only one reagent i.e. crystal violet‚ basic fuchsin‚ or methylene blue. Simple dyes used to stain bacteria have a positive charge cationic (+)‚ therefore‚ they are attracted to the bacteria that are usually negatively charged anionic (-) Depends
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testing in order to determine the identity of the unknown organism. The tests performed to identify the unknown organism included Gram Staining‚ Fluid thioglycollate/aero-tolerance test‚ Methyl Red-Vogues Proskauer‚ SIM test‚ Lysine Decarboxylase test‚ Lactose Fermentation test‚ Simmons Citrate test‚ and TSI test. The Gram staining technique showed that the organism was Gram negative and bacilli shaped. The fluid thioglycollate test showed that the organism was a facultative anaerobe‚ meaning ATP can
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Lab Practical 2 Morphological Staining Techniques Simple Stain- Uses 1 Stain 1. Acid Stains (- Charge)- Stains Background- Nigrosin‚ India Ink and Congo Red 2. Base Stains (+ Charge)- Stains Cell- Methylene Blue‚ Crystal Violet‚ Safranin Differential Staining Techniques- Any Staining Technique using 2 or more stains is differential. It allows us to differentiate between parts. 1. Gram Stain- Two Stains‚ PLUS Reagents- Distinguishes Chemical Composition of Cell Wall PG only (+ Purple)
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Gram Characteristics of Bacteria Introduction Bacteria are interesting little critters in that they are not easily visualized without some sort of stain. In classical methodology‚ two different kinds of stains have been used to identify bacteria: acidic and basic dyes. Acidic dyes are so called because they consist of a salt that has a cation that transfers no color‚ but the anion portion is colored and does give off color. Basic dyes consist of an anion that does not give off any color and a cation
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Microscopic observations were performed on four different microscope slides. Three of these observations were gram stains and the last observation was of an endospore staining. The unknown bacteria samples for the endospore stain were taken off of a nutrient agar plate and stained using the Bartholomew and Mittwer’s method. Gram stain samples were taken off of tryptic soy agar and stained using the gram stain procedure that is listed in the Willgoh and Bleakly manual (2). Physiological Tests All biochemical
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4. Procedures 5-6 5. Results 7 6. Discussion8-9 7. Conclusion 10 8. Acknowledgments 11 9. Bibliography 12 10. Appendix 13 11. Forms 14 Introduction The type of bacteria found in milk can help society in many ways. For example‚ if testing goat’s milk‚ sheep’s milk‚ skim milk‚ and two percent milk‚ and the results are similar‚ and then the basic structure of the milks DNA is similar. This conclusion can come in use when products are being tested on cows‚ sheep‚ and
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