bacteria? Gram Stain This separation is based on differences in what physical trait? The structure of the cell walls. What is the iodine used for in this staining technique? Crystal violet is first used to stain the sample and iodine is used to affix it. Why is it important to heat fix the bacteria prior to the staining procedure? This procedure is done to kill any bacteria that may be on the slide already and this will insure a clean slide. If you had a bacteria sample that you knew to be gram positive
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bacteria and applying it to the results. This helps to slowly eliminate any bacteria that do not correspond with the results. I obtained tube number twelve to run various tests on. There are only two microorganisms‚ one is Gram positive and one is Gram negative‚ inside the test tube. The Gram positive possibilities are to be narrowed down from Bacillus cereus‚ Clostridium perfringens‚ Corynebacterium xerosis‚ Enterococcus faecalis‚ Streptococcus agalactaie‚ Staphylococcus aureus‚ and
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colored colony. Gram-staining was utilized as a first step to differentiate between gram-positive and gram-negative. After determining that it was a Gram negative single bacillus‚ biochemical tests performed and their purpose and results are shown in table and flow chart form. Table 1: biochemical Test Results Test | Purpose | Reagents | Observations | Results | Gram stain | To determine the Gram reaction of the bacterium | Crystal violet‚ Iodine‚ Alcohol‚ Sarasin | Pink rod | Gram-negative rods
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bacteria E.coli was the only type found in the given area by gram staining. E.coli was the chosen bacteria for this type of experiment. It is a gram negative bacterium that will grow rapidly given ‘any culture medium with the necessary energy source‚ nutrients‚ pH‚ and temperature’. Therefore‚ MacConkey Agar being the medium for its growth will enable us to achieve the experiment outcome. MacConkey Agar only allows the growth of gram negative bacteria as they do not contain bile salts and crystal
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most important tests done was gram staining because it helped to limit the possible bacterium that it could have been. To be sure which bacterium was worked with we referred to the Bergey’s manual and compared the results to the possible bacterium. Results:
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incubated at room temperature showed red colonies throughout its media. This identified it as the gram-negative bacteria Serratia marcescens‚ but further tests had to be conducted to fully confirm. Bacteria A showed a white opaque growth in both of its plates. Secondly both bacteria had to be gram stained‚ so that they could be identified as gram-positive or as gram-negative. The same gram staining method was used for both bacteria‚ which consisted of inoculating each bacteria into a
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Question 1 of 12 2.0 Points All of the following are considered microbes except A.viruses B.bacteria C.protozoa Correct D.worms Answer Key: D Question 2 of 12 2.0 Points Microbiologists investigate problems by designing and carrying out experiments. What is true about the scientific method? A.a hypothesis is the definitive explanation to account for the observation and therefore does not need to be tested B.a prediction is the factor that can change
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how the bacteria function. Over a two week period‚ eight prepared types of test media were provided to identify the assigned unknown mixed cultures. Not all of these tests were performed on every culture‚ as some were used only for gram positive or gram negative bacteria. The tests performed and what constituted a positive or negative test are as follows: Lab day 1; today in lab we obtained the unknown mixed culture “041”and one brain-heart infusion agar (BHIA). The first step was the
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tube by running a series of experiments to detect which specific Gram negative organism we had. To detect your gram positive from the mixed culture was given as extra credit points also. A Gram stain was performed and isolation streak plate in order to isolate and observe the unknown organism. Before the series of test‚ a dichotomous key had to be written up in order to know what steps and tests to run to identify the unknown Gram negative organism. I had to run three experiments in all before I
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included: Gram stain‚ mannitol salt agar‚ coagulase tube test‚ and an antimicrobial susceptibility test. The tests selected were based on the results of a gram stain. Gram staining‚ the most commonly used differential stain‚ allows for the fast and easy detection between gram negative and gram positive organisms based on the presence or absence of peptidoglycan. A gram positive organism resists the decolorization process due to a large amount of peptidoglycan and appears a purple color. A gram negative
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