Exercise 5 Introduction: Many different species of bacteria look similar under the microscope and also have the same staining results (ex. Gram stain). To be able to differentiate between the different species‚ one can look at the metabolic differences (fermentation)‚ as well as the environmental condition differences (temperature‚ pH‚ oxygen requirements). Being able to manipulate these conditions in a controlled environment can help to correctly identify the exact bacteria. Different media
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and one result was inconclusive. The focus of this paper will be on the description of the just one of the three bacteria and some of its effects on humans. The first test done was a differential stain (gram stain) to determine the unknown microorganism cell shape and arrangement. The gram staining is done by first putting a sample of the unknown bacteria on a slide and it was heat fixed taking care not to over or under fixed the slide. The slide was flooded with crystal violet and let stand for 30
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inoculating loop or needle‚ Bunsen burner‚ glass slides‚ lens paper‚ bibulous paper‚ regular tap water‚ along with reagents Crystal violet‚ Gram’s Iodine‚ 95% ethyl alcohol‚ and safranin. The first test I conducted was the gram stain. In preparation for the actual gram staining process I had to make a smear. To do this‚ I acquired a clean glass slide. I then placed one drop of water onto the center of the slide. Using the flame from my Bunsen burner I sterilized my loop by passing the malleable
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colonies. Bacteria are classified on the basis of many characteristics. Morphological and physiological features such as cell shape‚ motility‚ formation of spores and other distinguishable structures‚ and reaction to Gram stain is a good start in identifying bacteria. Other staining techniques such as Acid Fast stain are also useful in determining species. More important in identification of a genus and species of bacteria are biological tests‚ including the determination of the types of nutrients
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Sarcina lutea‚ Pseudomonas fragi‚ Micrococcus luteus‚ Alcaligenes faecalis‚ Clostridium sporogenes‚ and Micrococcus roseus. There were several qualitative tests that could be conducted to determine the identity of the unknown species‚ for example‚ Gram staining‚ Fermentation‚ Catalase‚ Oxidase‚ Starch Hydrolysis‚ Litmus Milk‚ MOI medium‚ and the Gelatin Test. All tests and techniques used were performed in accordance with The Microbiology Lab Manual. Materials and Method: A stock broth culture of
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known that were mentioned above. First it was the Gram staining procedure used to categorize bacteria in two groups‚ gram positive and gram negative. Gram staining is one of the most useful test used in the clinical setting to identify bacterial colonies due to their broad staining spectrum. (Black‚ 2008‚ pp. 70-71) The basis of the Gram stain is that gram positive bacteria retains the color of the primary dye‚ the crystal violet‚ whereas the gram negative bacteria loses the primary dye once its
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the knowledge gained from the sessions in the laboratory‚ we can now integrate what we have learned to the process of finding out the unknowns given. Materials and Methods The professor gave out the unknown specimens. It contained one-‐gram positive and one-‐gram negative bacteria from the given list. I was assigned unknown A. The process of identification was achieved by utilizing procedures learnt during the semester. Procedures were followed as stated in the lab manual (1). Since the sample contained
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Microbiology Department of Microbiology University of Kwa-zulu Natal 25 October 2010 ABSTRACT Different types of bacteria in various forms are found all around us‚ and it is a microbiologist’s job to be able to identify these bacteria. Using various staining techniques and physiological tests‚ an isolated bacterium can be identified. In this experiment‚ a single bacterial colony was isolated form Mycorrhizal spores‚ and further tests done on that colony. Sub culturing was done after each week to ensure
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ATM (STP). A Gram stain was then carried out to differentiate the unknown sample from a broad class to a more specific category of bacteria. The Gram stain distinguishes between Gram-positive and Gram-negative bacteria based on the composition of the cell walls. Gram-positive bacteria appear purple and Gramnegative bacteria appear pink after staining. The first Gram stain produced unsatisfactory results and was then repeated with a clear indication of negativity. Light pink staining was evident
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two slides for gram staining and viewing under a microscope. {Either my gram-stain slides were bad or the microscopes I chose for viewing were not good. No bacteria were found under the microscope.} Prepare another gram stain of the unknown bacteria. {Gram-positive‚ coccus shaped bacteria were observed under the microscope. Also noted‚ the slide may have been inadvertently contaminated during the gram stain process by another person in the laboratory.} [I do think that the gram-stain result
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