Lab #3: Ion Exchange Chromatography Objective The purpose of this experiment was to separate proteins on the basis of their net charge at a particular pH. In cation exchange chromatography positively charged molecules are attracted to a negatively charged column. Conversely‚ in anion exchange chromatography‚ negatively charged molecules are attracted to a positively charged column. Experimental results could be monitored in a predictable way by controlling running pH‚ salt concentration‚ and by
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Column and Thin Layer Chromatography Beverly Abstract: Plant pigments were separated and concentrated from a crude spinach extract through the use of column chromatography and an eluatropic series of hexanes‚ hexane/acetone‚ and methanol. The pigments were analyzed using thin layer chromatography with a 30% ethyl acetate/hexane developing solvent. Introduction: Chromatography is a technique used to separate a mixture of two or more components based on
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Chromatography refers to a set of laboratory methods used in separating as well as purifying biomolecules. A variety of chromatography techniques exist‚ and all depend on the interaction between a stationary and a mobile state. Two types of chromatography methods were examined in this investigation. First‚ ion-exchange chromatography was used. This method separates ions and polar molecules based on their affinity to the ion exchanger [2]. Specifically‚ cation-exchange chromatography was performed
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Student ID: 4593119 Date: 7 Dec 2014 Course and Section Number: SCIN131 A004 Fall 14 Lesson 4 Lab: Chromatography and Ionic versus Covalent Bonds PART 1 Begin by viewing the following Thinkwell video 15.1.3 CIA Demonstration: Chromatography After you watch the above video‚ answer the questions below in sufficient detail: (a) (3 pts.) This video discusses 3 different types of chromatography. List each one mentioned‚ and describe their differences in as much detail as possible (your points
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in the BIOL 1F90 Laboratory Manual (Martin‚ 2013)‚ Experiment # 1‚ pages 2-5. Results: Table 1: Culture Sample Chromatography Rf Values Sample of Spot Distance from Origin (mm) Solvent Front (mm) Rf Value Color A 6.0 57.0 0.22 Bluish green B 17.5 56.0 0.105 Dark green blue C 4.0 57.0 0.070 Greenish blue D 4.0 47.0 0.085 Green E 10.5 56.5 0.186 Light blue Figure 1: Chromatography- TLC sheet From left to right- acetic acid‚ citric acid‚
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Separation techniques LIQUID CHROMATOGRAPHY ‘THE ART OF SEPARATION’ CHROMATOGRAPHY – AN INTRODUCTION Chromatography is a technique through which a mixture of chemical components are separated‚ identified and determined accurately. This technique while provides a way for analytical separations‚ also useful for preparative techniques by which pure compounds can be obtained. Detector Signal Blue Compound Sample Injection + Mobile Phase Retention Time Red Compound It is i defined d fi d as a
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Conclusion: (Identity‚ % error‚ other evidence‚ how to improve) The identity of Unknown A is the compound cobalt(II) chloride hexahydrate (CoCl₂•6H₂O).This is justified by the similarities in color of red-purple crystal like grains‚ which is significant in identifying the product out of the eight suggested identities. Only two had similar characteristics which were CoCl₂•6H₂O and CoSO₄•6H₂O. Before unknown A was heated‚ there was a notable common physical property of the substance being red crystal
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Performance Liquid Chromatography (HPLC) Instructor: Dr. Hüseyin BOZKURT High Performance Liquid Chromatography (HPLC) is one mode of chromatography‚ the most widely used analytical technique. Chromatographic processes can be defined as separation techniques involving mass-transfer between stationary and mobile phases. Present day liquid chromatography that generally utilizes very small packing particles and a relatively high pressure is referred to as high performance liquid chromatography (HPLC).
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“Poor governance leads to‚ and encourages and breeds‚ corruption in a number of ways‚ for instance through bribery and extortion‚ nepotism and fraud and embezzlement‚ It reduces the efficiency on which an economy depends‚ and by increasing the cost of investment‚ lowers the potential return. It also reduces the government’s resources and hence its capacity for investment. Common to other South Asian countries‚ corruption in Pakistan is unique because it occurs up stream‚ it has wings which
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Throughout the story the narrator writes about the wallpaper as being a grotesque yellow and she wishes to be moved to another room‚ but as she keeps writing her feelings change about the wallpaper it starts to grow on her. When she first arrives at the mansion and enters her the nursery she describes the wallpaper as being "almost revolting; a smouldering unclean yellow‚ strangely faded by the slow-turning sunlight‚" which illustrates she despises it and makes the assumption that the children before
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