Construction of a Standard Curve for the Determination of Protein Concentration 09/13/12 PCB 3023-007 Results Figure1: Standard curve. This graph was constructed from the fixed amounts of Bovine Serum Albumin (BSA). Varying amounts of BSA at a concentration of .5µ/µl were mixed with the water and 1mL of coomassie dye. The equation y=0.0259x +0.0511 represents the best fit line for a data set taken from µg of protein equals two to ten µg. Optical density (OD) was surveyed using a Mach
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1 Practical report: Determination of concentration using a standard curve Introduction Spectrophotometer is an instrument that confirms the measurement of specified wavelength of light that passes through medium. The measurement of light absorbance by a solution is also done with spectrophometer. Absorption spectrum Spectrometers are mainly and widely functional in identifying the components of solutions that helps determine the components’ concentrations. Compounds are experimentally identified
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Clinical Chemistry Manlangit‚ Joyce Ann U. EXPERIMENT Title: Standard Curve Preparation Objectives: 1. Properly set up a standard curve 2. Using the prepared curve‚ determine the concentrations for control and patient specimens. Materials and Reagents: 2ml 1M Sulfuric Acid 2ml of 0.1M Potassium Dichromate Distilled Water Pipettes Volumetric Flask Test Tubes Procedure: 1. Prepare 1 ml glucose solution and place in test tubes 2. Add 2ml of 1M Sulfuric Acid solution 3. Add
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Report 4 Biology 103 Prof. Dr. Johnson Spectrophotometer; the finding of protein concentration of an unknown sample of BSA‚ and by using the standard curve. Abstract A spectrophotometer’s purpose is to use colors of the light spectrum to determine the concentration of light absorbing molecules in a solution. (p.59) In this particular lab‚ our mission was to determine the protein concentration and the standard curve of the unknown sample of BSA. This‚ by preparing five dilutions of the unknown
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bell curve is literally‚ the symmetrical curve created on a graph when using a frequency distribution method for a set of data‚ splitting the mean symmetrically. There is a big difference between standard deviation and the bell curve! Standard deviation shows the difference in variation from the average; the bell curve‚ also normal distribution or Gaussian distribution‚ shows the standard deviation and is created by the normal or equal distribution of the mean among either half. The bell curve is an
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Winny Stephanie Experiment 1: Quantitative Determination of Protein Concentration Using the Biuret Test Table 1: Experimental protocol for construction of the protein standard curve Tube 1 2 3 4 5 6 Buffer (ml) 1.0 0.8 0.6 0.4 0.2 0.0 BSA Protein solution (10 mg ml-1) (ml) 0.0 0.2 0.4 0.6 0.8 1.0 Biuret reagent (ml) 4.0 4.0 4.0 4.0 4.0 4.0 Total Volume (ml) 5.0 5.0 5.0 5.0 5.0 5.0 Final protein concentration (mg ml-1) 0 2 4 6 8 10 Absorbance 0.000 0.092 0.163 0.272 0.363 0.474 Table
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Title Determination of Protein Content Using Kjedahl and Titration 2.0 Introduction Proteins are polymers. They are the source of dietary amino acids and are used for growth and maintenance of living systems. They are costlier sources of energy compared to carbohydrates and fats and hence the human body utilizes proteins mainly for biosynthesis rather than as an energy source‚ though the energy yield is 5 kcal/g of protein. Twenty different types of amino acids occur naturally in proteins. Proteins
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whole class is stated on Figure 2. Table 3 shows the measurement on the amount of strawberry DNA extracted. In Figure 1 and Figure 2‚ the data will be presented Standard Curve Graph. The relationship between the concentration of Methylene Blue and the absorbance is expected to be a direct proportion. Figure 1: Standard Curve of light absorbance by ten different concentrations of Methylene Blue. The data of the figure is collected by our group Mitochondria. The relationship between two
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2.4.4. Direct determination of saliva proteins Protein contaminated with nucleic acids absorbed the light at wavelength 280 nm and it absorbs much strongly at wavelength 205 nm when it is free from nucleic acids. The UV-visible spectrophotometer was used in determination of saliva proteins (Figure 2.2). Cold trichloroacetic acid (10 % w/v ) was added to the sample‚ centrifuged for 10 minutes to precipitate protein. The absorbance of a known volume
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The Spectrophotometer Determination Of Protein Concentrations And The Effects Sodium Dodecyl Sulphate And Triton X-100 Have On Protein Concentration. INTRODUCTION Spectroscopy is used as a collective term for all the analytical techniques based on the interaction of light and matter. Spectrophotometry is one of the branches of spectroscopy where we measure the absorption of light by molecules that are in a gas or vapour state or dissolved molecules/ions (Tombs‚ et.al‚ 1959). Spectroscopy is the
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