B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure in protein purification. B. Introduction I. Fast Protein Liquid Chromatography
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Learning Curve Theory is concerned with the idea that when a new job‚ process or activity commences for the first time it is likely that the workforce involved will not achieve maximum efficiency immediately. Repetition of the task is likely to make the people more confident and knowledgeable and will eventually result in a more efficient and rapid operation. Eventually the learning process will stop after continually repeating the job. As a consequence the time to complete a task will initially
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Determination has helped many well known brands and people become known . One of the most important keys to success is determination. There are many details‚ and aspects from texts‚ but there were three reasons that were evidenced from the articles The business of beanbags‚ Climbing Kilimanjaro‚ and The Mighty Macs that stood out. The first reason is that being motivated can benefit you in many ways. Motivation is a reason to do something‚ or take action. By being motivated‚ Lilian Wairimu Njue
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The Human Medicines Regulations 2012 PART 1- Contains provisions and an explanation of the role of ministers and licensing authorities PART 2- How different advisory bodies operate (including the commission on Human Medicines and the British Pharmacopoeia Commission PART 3- Rules for manufacturing‚ importing and wholesale dealing (requiring a legally obtained license) PART 4- Requirement for authorisation to sell/supply medicinal products‚ i.e. they must be from an appropriate marketing authorisation
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Name: Dai Wei ID Number: 088916 Environmental Kuznet Curve Environment Kuznet Curve is the application of Kuznet Curve which describes the relationship between the degree of income inequality and the stage of development. However‚ EKC depicts the variation of environmental degradation with the increasing incomes. The illustration in relation to the relationship resembles a reverse “U” shape as shown in figure 1.1. That means the harm to environment brought from economic development of
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TOTAL PRODUCT CURVE: A curve that graphically represents the relation between total production by a firm in the short run and the quantity of a variable input added to a fixed input. When constructing this curve‚ it is assumed that total product changes from changes in the quantity of a variable input (like labor)‚ while other inputs (like capital) are fixed. This is one of three key product curves used in the analysis of short-run production. The other two are marginal product curve and average product
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Experiment #2 “Density Determinations” Report The objective for experiment #2 was “to determine densities of objects/salt solutions with different concentrations of salt‚ to see how density changes as a function of concentration.” In experiment #2‚ part II‚ calculations of Density of NaCL solutions were made from 0%-25% NaCL concentration. My hypothesis was that as the % increased‚ so would the density‚ because adding weight would increase the density of each solution. The density calculations
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Where ’s the Protein? Proteins‚ which are constructed from amino acid monomers‚ are considered one of the four major macronutrients needed by the body‚ along with carbohydrates‚ lipids‚ and nucleic acids. Only the first three nutrients listed require delicate balancing. One must consider the sources from which he or she receives those required nutrients. Carbohydrates from fruits‚ vegetables‚ and grains; lipids from oily animals such as fish or plant foods like avocados; and proteins from meats‚
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SECTION 2: TIME VALUE OF MONEY Decision problems in business usually involve a decision over whether to accept one alternative over another‚ or whether to implement a plan or not. In most cases‚ the effects of these decisions are felt in the future. Examples: Expand into a new market (geographical‚ consumer segment‚ etc.) or not? Now or later? Outsource production or keep it in-house? Grow organically or acquire a competitor? Or don’t grow at all? Purchase shares in Microsoft
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Determination of KHP – Acid - Base Titration For determining purity of unknown KHP‚ acid – base titration was used. Molarity of titrant in this case NaOH was standardized as 0.04958(±0.00011) M. Purity of unknown KHP was calculated as 35.88(±0.16) %. • Introduction Acid – base titration is typical method of determining unknown figures. Acid – base titration means that by using neutralization between acid and base. Abrupt changing of pH says whether procedure is done or not. pH indicators
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