3.6.5. Southern hybridization Southern blot hybridization analysis was carried out using standard procedures (Sambrook et al. 2001). Approximately 20 µg of total genomic DNA from transgenic plants and untransformed control plant was digested with EcoRI‚ and then separated by electrophoresis on 0.8% agarose gel. Digestion profile was checked with 1µg DNA‚ with single cutter enzymes EcoRI (10U) in T-DNA region. For southern experiment‚ 20µg DNA was digested overnight. Restricted fragments were separated
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Catherine Ann Taylor Comparative Politics Spring 2013 The Relationship between the English and Northern Irish in the Context of In the Name of the Father The intertwined and complex history of England and Ireland dates back to the 12th century‚ when English barons seized Irish lands. This continued until the 1300s‚ at which point most of the land in Ireland was owned by English. Loyalty to England weakened when the Englishmen began identifying more in Ireland. In 1534‚ Henry VIII took
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Race notes-sep.18 * Southern slavery * Age of flexibility (1619-1680) * South Carolina Slave Majority * Slave codes -status of the mother -chattel slavery-slaves are not even people‚ no rights -miscegenation After the revolutionary war: Economics- economy based on agriculture in south‚ so slaves are key Land expansion Property rights Scientific racism * carl Linnaeus Haitian revolution (1791-1804) scares americans -Toussaint
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SLAVERY IN THE SOUTHERN COLONIES Slavery has had a big impact on the southern colonies. Between 1607-1775 slavery evolved in the British North American colonies due to economic factors-such as the slave trade‚ the use of cash crops‚ and the plantation system- Geographic factors-such as climate‚ diseases‚ and the Caribbean connection- and social factors-such as racism‚ bacons rebellion‚ and the headright system. Slavery definitely had some big economic factors influencing it. Slave trade was
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China Southern Airlines was founded in 1995 and is based in Guangzhou. It is China’s largest airline in terms of fleet size‚ with 309 aircraft. The airline employees over 45‚500 full time employees. Its main hubs include Guangzhou and Beijing‚ but it operates in over 150 cities throughout China. Furthermore‚ China Southern operates 616 domestic 82 routes‚ including 90 international routes and 25 routes in Hong Kong and Macau. As China’s only company that is in world top ten passenger airlines
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south is known for a lot of things‚ amazing sweet tea‚ good barbeque‚ but have you ever thought about southern hospitality? No‚ it isn’t one of Ludacris’s albums‚ well‚ not in this sense anyway. Southern Hospitality to me is defined as people being willing enough to volunteer their time‚ homes‚ churches‚ etc to the community‚ or even country. Tennessee in particular is well known for their southern hospitality‚ and willingness to volunteer. We’re called the Volunteer State because in the War of 1812
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In the fifth chapter‚ this thesis will assess the abolitionist effort to denounce the legitimacy of using the Bible to sanction southern slavery by arguing that biblical slavery was not based upon the inferiority of one race whereas southern slavery was based upon the inferiority of one race. In short‚ these abolitionists sought to highlight that southerners were using a book which sanctioned a system of slavery that was not based upon the inferiority of one race to sanction a system of slavery that
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Men vs. Women By Angelica G. Sto. Domingo Ever thought why and how are people different from each other? Their basic differences (which can also be their similarities) are intelligence‚ opinions‚ body structure‚ religion‚ responsibilities‚ priorities‚ goals‚ and personalities. But the most obvious of all these is their difference in gender (Sozdinler‚ 2008). According to Sozdinler‚ many ancient and modern philosophers say that people are born in equal conditions. They may have the same intelligence
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APPENDIX A Questionnaire Republic of the Philippines SOUTHERN LUZON STATE UNIVERSITY Gumaca Campus Gumaca‚ Quezon Software Acceptability and Features | Rating | | 1 | 2 | 3 | 4 | A. Functionality of the Propose System | | | | | 1. The system can accept organized data | | | | | 2. The system has the ability to be used by the admin‚ manager and user | | | | | 3. The system operates in real time | | | | | 4. The system prevents unauthorized person
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According to (Lim‚ 2006) the Northern Region is one of the four regions in Peninsular Malaysia‚ which include the states of Perlis‚ Kedah‚ Penang‚ and Perak. Regarding to economic development‚ Penang and Perak can be classified as middle-income states while Kedah and Perlis were being classified as low-income states. (Aslam & Hassan‚ 2003) With a population of 1.77 million Penang is the leader in manufacturing activities and the growth centre for northern Malaysia. In 2007‚ 200 multinational
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